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PIGN (NM_176787) Human Untagged Clone

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SKU
SC310041
PIGN (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1
  $939.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PIGN
Synonyms MCAHS; MCAHS1; MCD4; MDC4; PIG-N
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC310041 representing NM_176787.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCTGCTGTTCTTTACTTTGGGATTGCTTATACATTTTGTGTTCTTCGCCTCCATCTTTGACATTTAT
TTTACATCTCCTTTGGTTCATGGAATGACTCCTCAGTTTACACCATTGCCTCCTCCAGCGAGAAGATTA
GTGTTGTTTGTTGCTGATGGCCTTCGAGCAGATGCACTTTACGAATTAGATGAAAATGGAAACTCTAGA
GCACCGTTTATTAGGAATATCATAATGCATGAAGGCAGCTGGGGCATATCTCATACACGTGTGCCAACA
GAATCTCGGCCAGGTCATGTAGCTCTGATAGCTGGGTTTTATGAAGATGTCAGTGCAGTTGCCAAAGGA
TGGAAGGAAAATCCTGTAGAGTTTGATTCTCTTTTTAATGAAAGTAAATACACATGGAGCTGGGGAAGC
CCAGATATCCTGCCTATGTTTGCCAAAGGTGCTAGTGGAGACCACGTTTATACATATAGTTATGATGCT
AAAAGAGAGGATTTTGGTGCTCAAGATGCAACAAAACTGGATACGTGGGTTTTTGATAATGTTAAGGAC
TTCTTTCATCATGCCAGAAACAACCAGTCTTTGTTTTCTAAAATAAATGAAGAGAAAATAGTTTTTTTC
TTACATTTATTAGGAATAGATACAAACGGACATGCTCATCGACCATCCTCGAGAGACTACAAGCACAAT
ATTAAAAAAGTTGATGATGGAGTTAAAGAAATCGTGTCTATGTTTAACCACTTCTATGGAAATGATGGG
AAAACAACATTTATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCT
TCAGAGACTTTAACTCCTTTAGTCACTTGGGGAGCTGGAATCAAGTATCCCCAAAGAGTATCAGCTCAG
CAATTTGATGATGCATTTTTGAAAGAGTGGAGATTGGAGAATTGGAAGAGGCTAGATGTCAATCAGGCT
GATATTGCACCATTGATGACTTCCCTTATTGGAGTTCCCTTTCCTCTTAACTCAGTGGGAATCCTTCCT
GTGGATTATCTTAACAACACTGATCTCTTCAAAGCAGAGAGCATGTTTACAAATGCAGTACAGATTCTT
GAACAGTTCAAGGTGAAAATGACTCAGAAGAAAGAAGTTACTTTACCATTTTTGTTTACACCATTTAAA
CTGCTTTCTGATTCCAAACAGTTCAACATTTTAAGAAAAGCAAGATCTTATATAAAACACAGAAAGTTT
GATGAAGTGGTCTCCCTTTGCAAGGAGCTAATTCATCTTGCATTGAAAGGATTGTCCTATTATCACACA
TATGACAGATTCTTTTTGGGCGTCAATGTTGTTATTGGTTTTGTGGGATGGATATCTTATGCCTCTTTG
TTGATCATCAAGTCTCATTCCAACCTTATAAAAGGTGTTAGTAAAGAAGTGAAGAAACCAAGCCATCTC
CTGCCTTGTAGTTTTGTAGCTATTGGCATTTTAGTAGCATTTTTTCTGCTGATTCAAGCCTGTCCCTGG
ACATATTATGTATATGGTTTGTTGCCACTGCCAATATGGTATGCGGTTCTAAGAGAATTTCAAGTTATT
CAGGACCTTGTTGTATCAGTGTTGACCTATCCTCTGAGCCATTTTGTTGGGTACCTGTTAGCCTTTACC
CTGGGAATTGAAGTATTAGTTCTCAGTTTTTTCTACCGCTATATGCTTACCGCTGGACTTACTGCCTTT
GCAGCTTGGCCATTTCTCACTCGGCTGTGGACTCGAGCAAAGATGACCTCACTGAGTTGGACTTTCTTC
TCTTTGCTCCTGGCAGTGTTCCCACTGATGCCGGTTGTAGGTCGAAAGCCAGACATCTCTCTAGTGATG
GGTGCAGGCTTGCTGGTTCTTCTGTTATCCCTGTGTGTTGTAACATCTCTCATGAAAAGAAAAGATAGC
TTTATAAAGGAAGAGCTATTGGTACATCTGTTACAGGTGCTGAGCACAGTGCTCTCCATGTATGTTGTG
TATAGCACTCAGAGTAGTCTACTCAGGAAGCAAGGACTGCCTCTCATGAATCAAATTATTAGCTGGGCA
ACATTAGCCTCTTCCTTGGTTGTGCCACTACTGAGTTCTCCAGTTCTCTTTCAGCGATTGTTCAGCATA
CTTCTTTCATTGATGTCAACCTACCTACTTCTAAGCACAGGGTATGAAGCTCTCTTTCCACTAGTGTTG
TCTTGTTTGATGTTTGTCTGGATAAACATAGAACAAGAAACTCTACAACAATCTGGTGTTTGCTGTAAA
CAAAAGCTCACCAGTATCCAGTTCTCTTATAATACTGATATAACTCAGTTTCGACAGCTATATCTGGAT
GACATCCGTAGGGCCTTTTTCCTTGTTTTCTTCTTAGTGACAGCATTTTTTGGAACTGGAAATATAGCT
TCTATTAACAGCTTTGATCTTGCCTCTGTCTATTGCTTTCTGACTGTGTTCAGTCCTTTTATGATGGGA
GCCCTGATGATGTGGAAGATTTTAATCCCCTTTGTTCTTGTTATGTGTGCTTTTGAAGCAGTTCAGTTG
ACTACTCAGTTATCGTCAAAAAGCCTTTTTCTCATTGTTCTCGTCATATCAGACATTATGGCTTTGCAT
TTTTTCTTCTTGGTCAAGGATTATGGCAGCTGGCTTGATATTGGGACAAGCATCAGCCACTATGTGATT
GTCATGTCCATGACCATCTTTTTGGTGTTCCTCAATGGCCTGGCCCAGCTGCTCACAACGAAGAAACTC
AGACTATGTGGCAAACCCAAAAGTCACTTCATGTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_176787
Insert Size 2796 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_176787.4
RefSeq Size 4893 bp
RefSeq ORF 2796 bp
Locus ID 23556
UniProt ID O95427
Cytogenetics 18q21.33
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
MW 105.8 kDa
Summary This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is expressed in the endoplasmic reticulum and transfers phosphoethanolamine (EtNP) to the first mannose of the GPI anchor. Two alternatively spliced variants, which encode an identical isoform, have been reported. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the longer variant. Variants 1 and 2 encode the same isoform.
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Documents
Resources
"NM_176787" in other vectors (6)
SKU Description Size Price
RC205334 PIGN (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1 10 ug
$852.00
RC205334L1 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1, Myc-DDK-tagged 10 ug
$1,152.00
RC205334L2 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1, mGFP tagged 10 ug
$1,152.00
RC205334L3 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1, Myc-DDK-tagged 10 ug
$1,152.00
RC205334L4 Lenti ORF clone of Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1, mGFP tagged 10 ug
$1,152.00
RG205334 PIGN (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class N (PIGN), transcript variant 1 10 ug
$789.00 $1,052.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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