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DAGLA (NM_006133) Human Untagged Clone

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SKU
SC309995
DAGLA (untagged)-Human diacylglycerol lipase, alpha (DAGLA)
  $999.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DAGLA
Synonyms C11orf11; DAGL(ALPHA); DAGLALPHA; NSDDR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC309995 representing NM_006133.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCCGGGATCGTGGTGTTCCGGCGGCGCTGGTCTGTGGGCAGTGATGACCTCGTCCTACCGGCCATC
TTCCTCTTTCTCCTGCATACCACCTGGTTTGTGATCCTGTCCGTGGTGCTCTTCGGCCTGGTCTATAAC
CCGCACGAGGCCTGCTCCCTGAACCTGGTGGACCACGGCCGCGGCTACCTGGGCATCCTGCTGAGCTGC
ATGATCGCTGAGATGGCCATCATCTGGCTGAGCATGCGCGGGGGCATCCTCTACACGGAGCCCCGTGAC
TCCATGCAGTACGTGCTCTACGTGCGCCTGGCCATCCTGGTGATCGAGTTCATCTACGCCATCGTGGGC
ATCGTCTGGCTCACTCAGTACTACACCTCCTGCAACGACCTCACTGCCAAGAATGTCACCCTCGGAATG
GTTGTCTGCAACTGGGTAGTCATCCTCAGTGTGTGCATCACTGTCCTCTGCGTCTTCGACCCCACGGGC
CGCACCTTTGTCAAGCTGAGAGCCACCAAGAGGAGGCAGCGTAACCTGCGGACCTACAACCTGCGGCAC
CGCTTAGAGGAGGGTCAAGCCACCAGCTGGTCGCGCCGGCTCAAAGTGTTCCTCTGCTGCACGCGGACG
AAGGACTCCCAGTCAGATGCCTACTCAGAAATCGCCTACCTCTTTGCGGAGTTCTTCCGGGACCTTGAC
ATTGTGCCATCCGACATCATTGCTGGCCTGGTGCTGCTCCGGCAGCGGCAGCGGGCCAAGCGCAACGCC
GTGCTGGACGAGGCAAACAATGACATCTTGGCCTTCCTGTCTGGGATGCCGGTGACCAGAAACACCAAG
TACCTCGACCTCAAGAATTCACAAGAGATGCTCCGCTACAAAGAGGTCTGCTACTACATGCTCTTTGCC
CTGGCTGCCTACGGGTGGCCCATGTACCTGATGCGGAAGCCCGCCTGCGGCCTCTGCCAACTGGCTCGG
TCCTGCTCGTGTTGCCTGTGTCCTGCGAGGCCGCGGTTCGCCCCTGGAGTCACCATCGAGGAAGACAAC
TGCTGTGGCTGTAATGCCATTGCCATCCGGCGCCACTTCCTGGACGAGAACATGACTGCGGTGGACATC
GTCTATACCTCCTGCCATGATGCGGTCTATGAAACGCCCTTCTACGTGGCGGTGGACCATGACAAGAAG
AAAGTGGTGATCAGTATCCGGGGGACCCTGTCCCCCAAGGATGCCCTGACTGACCTGACGGGTGATGCT
GAGCGCCTCCCCGTGGAGGGGCACCACGGCACCTGGCTGGGCCACAAGGGTATGGTCCTCTCAGCTGAG
TACATCAAGAAGAAACTGGAGCAGGAGATGGTCCTGTCCCAGGCCTTTGGGCGAGACCTGGGCCGCGGA
ACCAAACACTACGGCCTGATTGTGGTGGGCCACTCCCTGGGCGCGGGCACTGCTGCCATCCTCTCCTTC
CTTCTGCGCCCACAGTATCCGACCCTCAAGTGCTTTGCCTACTCCCCGCCAGGGGGCCTGCTGAGTGAG
GATGCGATGGAGTATTCCAAGGAGTTCGTGACTGCTGTGGTTCTGGGCAAAGACCTCGTCCCCAGGATT
GGCCTCTCTCAGCTGGAAGGCTTCCGCAGACAGCTCCTGGATGTCCTGCAGCGAAGCACCAAGCCCAAA
TGGCGGATCATCGTGGGGGCCACCAAATGCATCCCCAAGTCGGAGCTGCCTGAGGAGGTAGAGGTGACC
ACCCTGGCCAGCACGCGGCTCTGGACCCACCCCAGCGACCTAACTATAGCCCTCTCAGCCAGCACTCCA
CTCTACCCGCCCGGCCGCATCATCCACGTGGTCCACAACCACCCTGCAGAGCAGTGCTGCTGCTGTGAG
CAGGAGGAGCCCACATACTTTGCCATCTGGGGCGACAACAAGGCCTTCAATGAGGTGATCATCTCGCCA
GCCATGCTGCATGAGCACCTGCCCTATGTGGTCATGGAGGGGCTCAACAAGGTGCTGGAGAACTACAAC
AAGGGGAAGACCGCTCTGCTCTCTGCAGCCAAGGTCATGGTGAGCCCTACCGAGGTGGACCTGACTCCT
GAGCTCATCTTCCAGCAGCAGCCACTCCCCACGGGGCCGCCCATGCCCACTGGCCTTGCCCTGGAGCTG
CCGACTGCAGACCACCGCAACAGCAGCGTCAGGAGCAAGTCCCAGTCTGAGATGAGCCTGGAGGGCTTC
TCGGAGGGGCGGCTGCTGTCGCCAGTGGTTGCGGCGGCGGCCCGCCAGGACCCGGTGGAGCTGCTGCTG
CTGTCTACCCAGGAGCGGCTGGCGGCGGAGCTGCAGGCCCGGCGGGCACCACTGGCCACCATGGAGAGC
CTCTCGGACACTGAGTCCCTGTACAGCTTCGACTCGCGCCGCTCCTCAGGCTTCCGCAGCATCCGGGGC
TCCCCCAGCCTCCACGCTGTGCTGGAGCGTGATGAAGGCCACCTCTTCTACATTGACCCTGCCATCCCC
GAGGAAAACCCATCCCTGAGCTCGCGCACTGAGCTGCTGGCGGCCGACAGCCTGTCCAAGCACTCACAG
GACACGCAGCCCCTGGAGGCGGCCCTGGGCAGTGGCGGCGTCACTCCTGAGCGGCCCCCCAGTGCTGCG
GCCAATGACGAGGAGGAAGAGGTTGGCGGTGGGGGTGGCGGGCCGGCCTCCCGCGGGGAGCTGGCGCTG
CACAATGGGCGCCTGGGGGACTCGCCCAGTCCTCAGGTGCTGGAATTCGCCGAGTTCATCGACAGCCTC
TTCAACCTGGACAGCAAGAGCAGCTCCTTCCAAGACCTCTACTGCATGGTGGTGCCCGAGAGCCCCACC
AGTGACTACGCTGAGGGCCCCAAGTCCCCCAGCCAGCAAGAGATCCTGCTCCGTGCCCAGTTCGAGCCC
AACCTGGTGCCCAAGCCCCCACGGCTCTTTGCCGGCTCAGCCGACCCCTCCTCGGGCATCTCACTCTCG
CCCTCCTTCCCGCTCAGCTCCTCGGGTGAGCTCATGGACCTGACGCCCACGGGCCTCAGTAGCCAGGAA
TGCCTGGCGGCTGACAAGATCCGGACTTCTACCCCCACTGGCCACGGAGCCAGCCCCGCCAAGCAAGAT
GAGCTGGTCATCTCAGCACGCTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_006133
Insert Size 3129 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006133.2
RefSeq Size 5761 bp
RefSeq ORF 3129 bp
Locus ID 747
UniProt ID Q9Y4D2
Cytogenetics 11q12.2
Protein Families Druggable Genome, Transmembrane
MW 115 kDa
Summary This gene encodes a diacylglycerol lipase. The encoded enzyme is involved in the biosynthesis of the endocannabinoid 2-arachidonoyl-glycerol.provided by RefSeq, Nov 2010
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"NM_006133" in other vectors (5)
SKU Description Size Price
RC214261 DAGLA (Myc-DDK-tagged)-Human diacylglycerol lipase, alpha (DAGLA) 10 ug
$589.00 $907.00
RC214261L3 Lenti-ORF clone of DAGLA (Myc-DDK-tagged)-Human diacylglycerol lipase, alpha (DAGLA) 10 ug
$1,207.00
RC214261L4 Lenti-ORF clone of DAGLA (mGFP-tagged)-Human diacylglycerol lipase, alpha (DAGLA) 10 ug
$1,207.00
RG214261 DAGLA (tGFP-tagged) - Human diacylglycerol lipase, alpha (DAGLA) 10 ug
$1,107.00
SC101029 DAGLA (untagged)-Human diacylglycerol lipase, alpha (DAGLA) 10 ug
$908.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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