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PCDHGB5 (NM_032099) Human Untagged Clone

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SKU
SC309667
PCDHGB5 (untagged)-Human protocadherin gamma subfamily B, 5 (PCDHGB5), transcript variant 2
  $825.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PCDHGB5
Synonyms PCDH-GAMMA-B5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC309667 representing NM_032099.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGGAGCGGCGCCGGGGAGCTGGGCCGGGCTGAGAGGCTGCCAGTGCTCTTTCTCTTCCTGCTGTCT
TTGTTCTGCCCGGCGCTCTGTGAGCAGATCCGCTACAGGATTCCCGAGGAAATGCCCAAGGGCTCCGTA
GTGGGGAACCTCGCCACGGACCTGGGGTTCAGCGTCCAGGAGTTACCGACTCGAAAACTGCGCGTCAGT
TCGGAGAAGCCTTACTTCACCGTGAGCGCAGAGAGCGGGGAGTTGCTTGTGAGCAGCAGGCTAGACAGG
GAGGAGATATGCGGGAAGAAGCCAGCTTGTGCTCTGGAATTTGAGGCTGTTGCTGAAAATCCACTGAAC
TTTTATCACGTGAATGTGGAGATCGAGGACATTAATGACCACACGCCAAAATTCACGCAAAATTCCTTT
GAGCTGCAAATAAGTGAGTCTGCACAGCCTGGCACAAGATTTATACTAGAAGTAGCAGAAGATGCAGAT
ATTGGCTTAAACTCTCTGCAGAAGTATAAACTCTCTCTTAACCCAAGTTTCTCATTAATAATTAAGGAG
AAACAGGATGGTAGTAAATACCCGGAACTGGCACTGGAGAAAACCTTAGACCGGGAACAACAGAGTTAC
CATCGTTTAGTCCTGACTGCCTTGGACGGTGGACATCCACCCCTAAGCGGCACCACTGAGCTCCGGATC
CAGGTAACCGACGCCAATGATAATCCCCCGGTATTCAACCGAGACGTGTACAGAGTCAGCCTTCGGGAA
AACGTGCCACCAGGCACCACTGTGTTGCAAGTGTCAGCCACTGACCAAGACGAGGGCATCAACTCAGAA
ATTACTTATTCCTTCTACAGAACCGGGCAAATCTTTAGTCTGAATTCAAAGAGCGGAGAAATTACCACT
CAAAAGAAACTGGATTTTGAAGAGACCAAGGAATATTCAATGGTTGTAGAAGGGAGGGATGGTGGTGGA
CTGGTTGCACAATGTACAGTTGAAATTAATATTCAAGATGAAAATGACAATAGCCCAGAAGTTACATTC
CATTCTCTACTTGAAATGATTCTGGAAAACGCGGTGCCTGGAACACTAATTGCTTTGATCAAAATACAT
GACCAAGATTCTGGGGAAAATGGGGAGGTTAATTGTCAATTACAAGGCGAAGTCCCTTTTAAGATTATC
TCTTCATCCAAAAATTCGTATAAGTTGGTAACAGATGGAACCCTAGACCGAGAGCAAACCCCGGAGTAC
AATGTCACCATCACAGCCACAGACAGGGGCAAGCCGCCCCTCTCCTCCAGCATAAGCGTCATCCTACAT
ATCAGAGACGTCAACGATAACGCTCCGGTTTTCCACCAGGCGTCCTACTTAGTCAGTGTACCCGAAAAC
AACCCTCCTGGGGCCTCCATCGCGCAAGTCTGCGCCTCGGACCTGGACTTGGGGTTGAACGGCCAAGTC
TCCTACTCTATCATGGCCAGCGACCTAGAGCCTCTGGCACTGGCCTCTTACGTGTCCATGAGCGCGCAA
AGTGGGGTGGTGTTCGCGCAGCGCGCCTTTGACTACGAGCAGCTGCGCACCTTCGAACTCACACTACAG
GCCCGCGACCAGGGCTCGCCTGCGCTCAGCGCAAACGTGAGCCTGCGCGTGTTGGTGGGCGACCGAAAC
GACAACGCACCGCGGGTGCTGTACCCCGCGCTGGGTCCCGACGGCTCTGCGCTCTTCGATATGGTGCCG
CGCGCTGCAGAGCCCGGCTACCTGGTGACCAAGGTAGTGGCCGTGGACGCAGACTCAGGACACAACGCC
TGGCTGTCCTACCACGTGCTGCAGGCTAGCGAGCCCGGGCTCTTCAGCCTGGGGCTGCGCACAGGAGAG
GTGCGCACAGCGCGTGCCTTGGGCGACAGGGACGCGGCCCGCCAGCGCCTGCTGGTTGCTGTGCGTGAT
GGTGGACAGCCGCCACTCTCCGCCACCGCCACGCTGCACTTGGTCTTTGCTGACAGCTTGCAGGAGGTG
CTGCCGGATATCACTGACCGCCCTGTACCCTCTGACCCCCAGGCTGAGCTGCAGTTTTACCTAGTGGTG
GCCTTGGCCTTGATCTCAGTGCTCTTCCTCCTGGCCGTGATTCTGGCCGTTGCCTTGCGCCTGCGACGC
TCCTCCAGCCCTGCCGCCTGGAGCTGCTTCCAACCTGGTCTCTGTGTCAAGTCTGGACCTGTGGTTCCC
CCCAACTACAGTCAGGGGACTTTGCCTTATTCCTACAACCTATGTGTTGCACATACAGGAAAGACGGAG
TTTAATTTCCTAAAATGTAGTGAGCAATTGAGTTCAGGACAAGACATACTTTGTGGTGATTCATCTGGG
GCCTTATTTCCACTTTGTAATTCCAGCGAGTCGACTTCCCATCCTGAGTTGGTGAGTTTCATTTATGTC
TATTCTTTTTCATTACCCACCCAATTTTCTGTATTTACATGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_032099
Insert Size 2457 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032099.1
RefSeq Size 2457 bp
RefSeq ORF 2457 bp
Locus ID 56101
UniProt ID Q9Y5G0
Cytogenetics 5q31.3
Domains CA
MW 89.2 kDa
Summary This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) utilizes the large, first exon then continues into the downstream intron 1 sequence before terminating. This one-exon transcript encodes the shorter isoform (2).
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"NM_032099" in other vectors (4)
SKU Description Size Price
RC210867 PCDHGB5 (Myc-DDK-tagged)-Human protocadherin gamma subfamily B, 5 (PCDHGB5), transcript variant 2 10 ug
$749.00
RC210867L3 Lenti ORF clone of Human protocadherin gamma subfamily B, 5 (PCDHGB5), transcript variant 2, Myc-DDK-tagged 10 ug
$1,049.00
RC210867L4 Lenti ORF clone of Human protocadherin gamma subfamily B, 5 (PCDHGB5), transcript variant 2, mGFP tagged 10 ug
$1,049.00
RG210867 PCDHGB5 (tGFP-tagged) - Human protocadherin gamma subfamily B, 5 (PCDHGB5), transcript variant 2 10 ug
$949.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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