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EEA1 (NM_003566) Human Untagged Clone

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SKU
SC309001
EEA1 (untagged)-Human early endosome antigen 1 (EEA1)
  $1,208.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EEA1
Synonyms MST105; MSTP105; ZFYVE2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_003566 edited
GGCACGAGGCGCTCGCAGGCTGCCTAGTCGTTCGCTCGCTCTCTCTCGCGTGCTCCCTCT
CGCCGCCTGTGGGGAAGTCGGGGCCGCCCGAGCGGCCGTCGCCACCGCCTCGCCCCGACC
TCCTTCCCGGTCCCCTCCCGGCCCAGTCCCTCCCGCCCGCGCACCCCGAGTAGTGAGTGG
CCCCGCGCAGGGTCTGGAGAGTCACCGCGGCGGCGCCGGGTGGTGGTTAAACCATGTTAA
GGAGGATTTTACAGAGGACTCCTGGGAGAGTTGGCTCTCAAGGTTCTGATTTAGATTCAT
CAGCAACTCCTATAAACACAGTGGACGTCAATAATGAAAGCTCTTCAGAGGGTTTCATAT
GTCCCCAGTGTATGAAATCTCTTGGATCTGCTGATGAACTTTTCAAACATTATGAAGCTG
TTCATGATGCTGGTAATGACTCAGGTCACGGAGGAGAGTCTAATCTTGCTTTGAAGCGAG
ATGATGTAACACTGCTCAGACAAGAGGTCCAAGACCTACAGGCTTCACTTAAGGAAGAAA
AATGGTACTCGGAAGAATTAAAGAAGGAATTAGAAAAATATCAAGGGCTGCAGCAGCAAG
AGGCCAAACCTGATGGGTTGGTGACTGATTCATCAGCAGAACTACAGTCTTTGGAACAGC
AATTAGAAGAAGCCCAAACAGAAAATTTTAATATTAAGCAAATGAAAGACTTATTTGAAC
AGAAAGCAGCCCAACTTGCTACTGAAATTGCAGATATAAAGTCAAAGTATGATGAAGAAA
GGAGTCTTCGAGAAGCTGCTGAACAAAAAGTGACACGTCTGACAGAAGAATTAAACAAAG
AGGCAACTGTAATTCAAGATCTGAAGACGGAACTGCTTCAGAGACCTGGTATAGAAGATG
TTGCCGTGCTAAAGAAAGAACTGGTCCAAGTTCAAACACTAATGGATAACATGACCTTGG
AACGTGAGCGAGAATCTGAAAAACTCAAAGATGAATGCAAAAAATTGCAGTCACAATATG
CTAGCTCAGAGGCCACAATAAGCCAGCTAAGGAGTGAACTTGCCAAAGGCCCCCAGGAAG
TTGCTGTATATGTACAGGAACTACAAAAACTGAAAAGTTCAGTTAATGAATTAACACAAA
AAAATCAGACCTTGACAGAAAACTTGCTGAAAAAAGAACAAGACTATACTAAGTTAGAGG
AGAAACATAATGAAGAATCTGTGAGTAAAAAGAATATTCAGGCAACCCTTCATCAAAAAG
ACCTAGATTGTCAACAGCTTCAGTCAAGATTGTCTGCATCTGAAACCTCACTGCATAGAA
TACATGTAGAACTAAGTGAAAAAGGAGAAGCTACTCAAAAGCTCAAAGAAGAATTATCTG
AGGTAGAGACCAAGTACCAGCATCTAAAGGCGGAGTTTAAGCAGCTACAACAACAGAGAG
AAGAAAAGGAGCAGCATGGGTTACAACTCCAAAGTGAAATTAATCAATTACATAGCAAAC
TTCTGGAGACAGAGCGCCAACTAGGGGAAGCTCATGGTAGGCTGAAGGAACAGAGACAGC
TTTCAAGTGAAAAGTTGATGGATAAAGAACAACAAGTGGCTGATTTACAACTCAAACTTT
CTCGGTTAGAAGAGCAGTTGAAGGAAAAAGTTACAAATTCTACAGAATTGCAGCATCAAT
TAGATAAAACAAAGCAACAGCATCAAGAACAACAGGCTCTTCAGCAAAGCACCACGGCAA
AACTTCGAGAAGCTCAGAATGATTTGGAACAAGTTCTACGTCAAATTGGCGATAAGGACC
AAAAGATCCAGAACCTTGAAGCTTTATTACAGAAGAGTAAAGAAAATATTTCATTACTAG
AAAAAGAAAGAGAAGATCTTTATGCAAAAATTCAGGCTGGTGAAGGAGAGACTGCTGTTC
TTAACCAGTTACAAGAAAAAAACCATACACTACAGGAGCAAGTAACTCAACTAACAGAGA
AGCTGAAGAATCAGTCAGAAAGTCATAAACAAGCCCAGGAGAATTTGCATGACCAGGTAC
AAGAGCAGAAGGCACATCTTAGAGCTGCACAAGACCGTGTCCTTTCCCTAGAAACTAGTG
TCAATGAATTAAATAGTCAATTAAATGAAAGCAAGGAGAAGGTCTCCCAGCTTGACATAC
AGATTAAAGCCAAAACCGAACTATTACTATCAGCAGAAGCAGCAAAAACTGCTCAAAGAG
CTGATCTTCAGAATCATTTGGACACAGCTCAAAATGCATTACAAGATAAACAGCAGGAGT
TAAATAAGATTACTACTCAGTTGGATCAGGTCACTGCAAAGTTACAAGACAAGCAAGAAC
ATTGCAGTCAGCTGGAAAGTCATCTTAAAGAATATAAAGAGAAATACCTCTCTTTAGAAC
AGAAAACCGAAGAGCTAGAAGGTCAAATTAAGAAACTAGAAGCTGATAGTCTTGAAGTTA
AAGCAAGCAAGGAGCAGGCTTTGCAAGATCTACAACAGCAAAGACAGCTGAACACAGATT
TAGAGCTCAGAGCCACAGAATTGAGTAAACAACTTGAAATGGAAAAGGAAATAGTATCCA
GTACAAGATTGGATCTACAGAAAAAATCTGAAGCCCTTGAAAGTATCAAGCAAAAGCTTA
CCAAGCAAGAGGAAGAAAAACAAATCCTGAAACAAGATTTTGAAACTTTAAGTCAAGAAA
CAAAGATTCAGCATGAGGAATTGAATAACAGAATTCAAACAACAGTAACAGAACTACAAA
AAGTGAAAATGGAGAAAGAAGCTTTAATGACAGAGCTTTCTACAGTAAAGGACAAACTAT
CAAAAGTTTCTGATTCTTTGAAAAACTCTAAAAGTGAATTTGAAAAGGAGAATCAGAAAG
GAAAAGCCGCTATATTAGACTTGGAAAAAACTTGCAAAGAATTAAAGCATCAACTTCAAG
TGCAGATGGAAAACACACTTAAGGAACAGAAGGAACTGAAAAAGTCACTTGAAAAAGAGA
AGGAGGCTTCTCATCAGTTGAAATTGGAACTCAATTCAATGCAGGAACAACTTATACAGG
CCCAGAATACTTTAAAACAAAATGAAAAAGAAGAGCAACAACTTCAGGGGAACATAAATG
AGCTAAAGCAATCAAGTGAACAGAAGAAAAAACAAATTGAAGCACTCCAAGGAGAGCTTA
AAATTGCTGTTTTACAGAAGACAGAGCTTGAGAATAAACTACAGCAGCAGTTAACACAGG
CAGCCCAGGAACTTGCAGCAGAGAAAGAGAAAATATCAGTATTACAAAACAACTATGAAA
AAAGTCAGGAAACTTTCAAACAGCTTCAATCTGATTTCTATGGGAGGGAATCTGAACTTC
TAGCCACCAGGCAAGATCTTAAGTCTGTAGAAGAGAAGCTTTCTCTAGCACAGGAGGACT
TGATTTCAAACAGAAATCAAATTGGAAATCAAAATAAATTGATTCAAGAACTGAAGACTG
CCAAGGCTACATTGGAGCAGGATTCAGCAAAGAAAGAACAGCAATTGCAGGAGCGATGTA
AAGCACTACAAGACATTCAGAAAGAAAAGTCACTGAAAGAAAAAGAACTGGTAAATGAGA
AGTCTAAATTGGCAGAGATAGAAGAAATTAAATGTAGACAAGAAAAAGAAATCACTAAAC
TAAACGAAGAACTCAAGTCCCACAAACTAGAAAGCATAAAGGAGATAACAAATCTTAAAG
ATGCTAAACAGCTTCTAATTCAGCAGAAATTAGAACTTCAAGGAAAAGCGGACTCCCTGA
AGGCAGCTGTTGAACAGGAGAAGAGAAATCAGCAGATACTAAAAGACCAGGTGAAAAAGG
AAGAAGAGGAGCTGAAGAAAGAATTTATTGAGAAAGAAGCTAAGTTGCATTCCGAAATAA
AAGAAAAGGAAGTAGGAATGAAGAAGCATGAAGAAAATGAGGCTAAACTTACCATGCAGA
TTACAGCATTAAATGAAAACTTAGGCACTGTGAAGAAGGAGTGGCAATCTAGTCAACGGA
GAGTTAGTGAGCTTGAGAAACAAACGGATGACTTACGGGGTGAAATTGCAGTATTAGAAG
CAACGGTTCAGAATAATCAAGATGAAAGGAGAGCACTACTGGAAAGATGTCTTAAAGGAG
AAGGTGAAATAGAAAAGCTTCAAACCAAAGTATTAGAATTGCAAAGAAAGCTGGATAATA
CAACTGCAGCAGTGCAGGAGCTGGGCAGAGAAAACCAATCACTTCAGATCAAACATACAC
AAGCGTTGAATAGAAAGTGGGCCGAAGACAATGAAGTACAAAACTGTATGGCCTGTGGGA
AAGGCTTTTCAGTAACAGTGAGACGGCATCACTGCCGACAGTGTGGAAATATCTTCTGTG
CTGAATGTTCAGCCAAAAATGCCTTAACTCCTTCCTCCAAGAAGCCTGTTCGTGTCTGTG
ATGCATGTTTCAATGACTTGCAAGGATAATGGGTTATCACAACTTCAGAGTAATATTACA
CTAACATTAGATTTTTAATAAATGTACTTAATAGAGGTCTTGGACTACTATTTGGTTTGG
ACACTGGTTGCAATACTAGACCAAATAGGAATTAGTATA:TTTTGGAATGTTATGGATAT
CAAGTAAAACTCTTCTATTTTTGTGAGACTTGGGCTTATCATCTTTCATTACTTTTTTCC
CATTTAGCCCCTGGAATAGCTTTCATGCCAAGTTTAGAATTAGCCAATGAAATGTAAATA
AACTTTGGACAGAAAATAGCAATGTATTTTTTTAATATAATTTCATTATTCACAAATGAA
GAATGCAATTCCATAGCTTACTTCTTTTTCCTAAATTTAATGTACAAAAATGTACATGGA
TGATATTATCACTTGTTGCTGTTTTTGTTGCACAATAGCACATTAATTATATTAAATATT
CTCTTTGTGAAGTTATGCACAGAACTGTAACACTTTCCTCTGCCTTTTTTTTTTATATGT
GGGTTCATGGTTCAGTTGCATTGTTATGTTATGAATTTACTACTTGGGTGTTTTAAATAC
AGTTTATTTTAAAATATGTAGTAATATGAATTCAAAAATGGTTTTTGTTTTAACAATATT
AATTAAATTTTTTTGGGTGGATTTTATAAAAATCTCTGTAAAATCAATTTGTTTACTTAT
AGTTATTTGGGGTTGTATTGCAAAGAATAGGGTTAATTTTTCTTTTTGTCTTTTTCTGTC
TCTTTTTTAAACTCCCACGCATTGTCCATTTAGAATTTTAGAAAGCTTTTTACCCTCGCT
GTCAGTTGGAGGATGAATAATAAACATTGTATAATGTTAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_003566
Insert Size 5300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be a perfect match to NM_003566.2.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003566.2, NP_003557.2
RefSeq Size 4874 bp
RefSeq ORF 4236 bp
Locus ID 8411
UniProt ID Q15075
Cytogenetics 12q22
Domains FYVE, M, zf-C2H2
Protein Pathways Endocytosis
Summary Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_003566" in other vectors (6)
SKU Description Size Price
RC215396 EEA1 (Myc-DDK-tagged)-Human early endosome antigen 1 (EEA1) 10 ug
$589.00 $1,207.00
RC215396L1 Lenti-ORF clone of EEA1 (Myc-DDK-tagged)-Human early endosome antigen 1 (EEA1) 10 ug
$1,507.00
RC215396L2 Lenti-ORF clone of EEA1 (mGFP-tagged)-Human early endosome antigen 1 (EEA1) 10 ug
$1,507.00
RC215396L3 Lenti-ORF clone of EEA1 (Myc-DDK-tagged)-Human early endosome antigen 1 (EEA1) 10 ug
$1,507.00
RC215396L4 Lenti-ORF clone of EEA1 (mGFP-tagged)-Human early endosome antigen 1 (EEA1) 10 ug
$1,507.00
RG215396 EEA1 (tGFP-tagged) - Human early endosome antigen 1 (EEA1) 10 ug
$789.00 $1,407.00

file-alt Citations

The p97 ATPase associates with EEA1 to regulate the size of early endosomes
Ramanathan, Ye
Cell Res. (2012) 22 (2), 346-59 DOI: 10.1038/cr.2011.80
Cited by 103
AI Summary: OriGene’s full-length human EEA1 protein was used in this study to investigate the interaction between p97 ATPase and EEA1, helping to elucidate p97’s role in regulating early endosome size.
Snippet: Compared with the gene-silencing approach, chemical inhibitors can rapidly shut down protein function and thus allow observation of the immediate effect associated with loss of protein function. ... Full-length human EEA1 was purchased (OriGene).
Showing 1-1 of 1 papers.
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