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Dopamine D2 Receptor (DRD2) (NM_016574) Human Untagged Clone

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SKU
SC308892
DRD2 (untagged)-Human dopamine receptor D2 (DRD2), transcript variant 2
  $457.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Dopamine D2 Receptor
Synonyms D2DR; D2R
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_016574 edited
GGGCGGCCGCGAATTCGGCACGAGCCGCCCCGCCGCGGCCCGTCCACTGCTCCCCGCGGG
CCAGAGCCGGCCGAGCTGCTGCCCGCCGGGGCTCTGAACGGCGCGGCGGGGCCGGGAGCC
AGGGACCGGCCGAGGAGAGTGGCGGCCCCGGACGGCTGCCGGAGGGGCGGCCGCGCGTGG
ATGCGGCGGGAGCTGGAAGCCTCAAGCAGCCGGCGCCGTCTCTGCCCCGGGGCGCCCTAT
GGCTTGAAGAGCCTGGCCACCCAGTGGCTCCACCGCCCTGATGGATCCACTGAATCTGTC
CTGGTATGATGATGATCTGGAGAGGCAGAACTGGAGCCGGCCCTTCAACGGGTCAGACGG
GAAGGCGGACAGACCCCACTACAACTACTATGCCACACTGCTCACCCTGCTCATCGCTGT
CATCGTCTTCGGCAACGTGCTGGTGTGCATGGCTGTGTCCCGCGAGAAGGCGCTGCAGAC
CACCACCAACTACCTGATCGTCAGCCTCGCAGTGGCCGACCTCCTCGTCGCCACACTGGT
CATGCCCTGGGTTGTCTACCTGGAGGTGGTAGGTGAGTGGAAATTCAGCAGGATTCACTG
TGACATCTTCGTCACTCTGGACGTCATGATGTGCACGGCGAGCATCCTGAACTTGTGTGC
CATCAGCATCGACAGGTACACAGCTGTGGCCATGCCCATGCTGTACAATACGCGCTACAG
CTCCAAGCGCCGGGTCACCGTCATGATCTCCATCGTCTGGGTCCTGTCCTTCACCATCTC
CTGCCCACTCCTCTTCGGACTCAATAACGCAGACCAGAACGAGTGCATCATTGCCAACCC
GGCCTTCGTGGTCTACTCCTCCATCGTCTCCTTCTACGTGCCCTTCATTGTCACCCTGCT
GGTCTACATCAAGATCTACATTGTCCTCCGCAGACGCCGCAAGCGAGTCAACACCAAACG
CAGCAGCCGAGCTTTCAGGGCCCACCTGAGGGCTCCACTAAAGGAGGCTGCCCGGCGAGC
CCAGGAGCTGGAGATGGAGATGCTCTCCAGCACCAGCCCACCCGAGAGGACCCGGTACAG
CCCCATCCCACCCAGCCACCACCAGCTGACTCTCCCCGACCCGTCCCACCACGGTCTCCA
CAGCACTCCCGACAGCCCCGCCAAACCAGAGAAGAATGGGCATGCCAAAGACCACCCCAA
GATTGCCAAGATCTTTGAGATCCAGACCATGCCCAATGGCAAAACCCGGACCTCCCTCAA
GACCATGAGCCGTAGGAAGCTCTCCCAGCAGAAGGAGAAGAAAGCCACTCAGATGCTCGC
CATTGTTCTCGGCGTGTTCATCATCTGCTGGCTGCCCTTCTTCATCACACACATCCTGAA
CATACACTGTGACTGCAACATCCCGCCTGTCCTGTACAGCGCCTTCACGTGGCTGGGCTA
TGTCAACAGCGCCGTGAACCCCATCATCTACACCACCTTCAACATTGAGTTCCGCAAGGC
CTTCCTGAAGATCCTCCACTGCTGACTCTGCTGCCTGCCCGCACAGCAGCCTGCTTCCCA
CCTCCCTGCCCAGGCCAGCCAGCCTCACCCTTGCGAACCGTGAGCAGGAAGGCCTGGGTG
GATCGGCCTCCTCTTCACCCCGGCAGGCCCTGCAGTGTTCGCTTGGCTCCATGCTCCTCA
CTGCCCGCACACCCTCACTCTGCCAGGGCAGTGCTAGTGAGCTGGGCATGGTACCAGCCC
TGGGGCTGGGCCCCCCAGCTCAGGGGCAGCTCATAGAGTCCCCCCTCCCACCTCCAGTCC
CCCTATCCTTGGCACCAAAGATGCAGCCGCCTTCCTTGACCTTCCTCTGGGGCTCTAGGG
TTGCTGGAGCCTGAGTCAGGGCCCAGAGGCTGAGTTTTCTGTTTGTGGGGCTTGGCGTGG
AGCAGGCGGTGGGGAGAGATGGACAGTTCACACCCTGCAAGGCCCACAGGAGGCAAGCAA
GCTCTCTTGCCGAGGAGCCAGGCAACTTCAGTCCTGGGAGACCCATGTAAATACCAGACT
GCAGGTTGGACCCCAGAGATTCCCAAGCCAAAAACCTTAGCTCCCTCCCGCACCCCGATG
TGGACCTCTACTTTCCAGGCTAGTCCGGACCCACCTCACCCCGTTACAGCTCCCCAAGTG
GTTTCCACATGCTCTGAGAAGAGGAGCCCTCATCTTGAAGGGCCCAGGAGGGTCTATGGG
GAGAGGAACTCCTTGGCCTAGCCCACCCTTCTGCCTTCTGACGGCCCTGCAATGTATCCC
TTCTCACAGCACATGCTGGCCAGCCTGGGGCCTGGCAGGGAGGTCAGGCCCTGGAACTCT
ATCTGGGCCTGGGCTAGGGGACATCAGAGGTTCTTTGAGGGACTGCCTCTGCCACACTCT
GACGCAAAACCACTTTCCTTTTCTATTCCTTCTGGCCTTTCCTCTCTCCTGTTTCCCTTC
CCTTCCACTGCCTCTGCCTTAGAGGAGCCCACGGCTAAGAGGCTGCTGAAAACCATCTGG
CCTGGCCTGGCCCTGCCCTGAGGAAGGAGGGGAAGCTGCAGCTTGGGAGAGCCCCTGGGG
CCTAGACTCTGTAACATCACTATCCATGCACCAAACTAATAAAACTTTGACGAGTCACCT
CCAGGAAAAAAAAAAAAAAGGGAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_016574 unedited
TTACATCGCCGACCCGTTGTCCCATTGGGCGGTAGGCGTGTGCGGTGGGCAGGTCTATAT
AAGCAGAGCTCGTTTAGTGAACCGTCAGATTTTGTAATACGACTCACTATAGGGCGGCCG
CGAATTCGGCACGAGCCGCCCCGCCGCGGCCCGTCCACTGCTCCCCGCGGGCCAGAGCCG
GCCGAGCTGCTGCCCGCCGGGGCTCTGAACGGCGCGGCGGGGCCGGGAGCCAGGGACCGG
CCGAGGAGAGTGGCGGCCCCGGACGGCTGCCGGAGGGGCGGCCGCGCGTGGATGCGGCGG
GAGCTGGAAGCCTCAAGCAGCCGGCGCCGTCTCTGCCCCGGGGCGCCCTATGGCTTGAAG
AGCCTGGCCACCCAGTGGCTCCACCGCCCTGATGGATCCACTGAATCTGTCCTGGTATGA
TGATGATCTGGAGAGGCAGAACTGGAGCCGGCCCTTCAACGGGTCATACGGGAAGGCGGA
CAGACCCCACTACAACTACTATGCCACACTGCTCACCCTGCTCATCGCTGTCATCGTCTT
CGGCAACGTGCTGGTGTGCATGGCTGTGTCCCGCGAGGAGGCGCTGCAGACCACCACCAA
CTACCTGATCGTCAGCCTCACAGTGGCCGACCTCCTCGTCGCCACACTGGTCATGCCCTG
GGTTGTCTACCTGGAAGTGGTAAGTGAGTGGAAAATCAGCAGCATCACTGTGACATCTTC
ATCACTCCGGGCGTCATGATGTGCACGGGCGAGCCTTCTGAACCTGTGGTGCCATAAGCA
TCGAAGGTACCCAAATGGGGCCCATGCCCACTGCTGGAAATAACTCGCTAC
3' Read Nucleotide Sequence
>OriGene 3' read for NM_016574 unedited
NTGTTGGTGGCTNCTTCNGATTTTATTAGTTTGGTGCATGGCTAGTGATGTTACAGTAGT
CTAAGGCCCCAGGGGTCTCTCCCAAGCTGCAGCTTCCCCTCCTTCCTCAGGCAGGGCCAG
GCCAGGCCAGATGGTTTTCAGCAGCCTCTTAGCCGTGGGCTCCTCTAAGGCAGAGGCAGT
GGAAGGGAAGGGAAACAGGAGAGAGGAAAGGCCAGAAGGAATAGAAAAGGAAAGTGGTTT
TGCGTCAGAGTGTGGCAGAGGCAGTCCCTCAAAGAACCTCTGATGTCCCCTAGCCCAGGC
CCAGATAGAGTTCCAGGGCCTGACCTCCCTGCCAGGCCCCAGGCTGGCCAGCATGTGCTG
TGAGAAGGGATACATTGCAGGGCCGTCAGAAGGCAGAAGGGTGGGCTAGGCCAAGGAGTT
CCTCTCCCCATAGACCCTCCTGGGCCCTTCAAGATGAGGGCTCCTCTTCTCAGAGCATGT
GGAAACCACTTGGGGAGCTGTAACGGGGTGAGGTGGGTCCGGACTAGCCTGGAAAGTAGA
GGTCCACATCGGGGTGCGGGAGGGAGCTAAGGTTTTTGGCTTGGGAATCTCTGGGGTCCA
ACCTGCAGTCTGGTATTTACATGGGTCTCCCANGACTGAAGTTGCCTGGCTCCTCGGCAA
GAGAGCTTGCTTGCCTCCTGTGGGCCTTGCAAGGTGTGAACTGTCCATCTCTTCCCACCG
CCTGCTTCAAGGCCAGGCCCACAAACAGAAAACTCAGCCTCTTGGGCCCTGACTCCAGCT
CCAGCAACCCCTAGAGCCCCCAAAGGAAGGTCAAGGAAAGGCGGCTTGCTCTTTGGTGCC
CAGGGATAGGGGGACTGGAGGTGGGAAGGGGGGACTTTATAAGCTGCCCCTGGACTGGGG
GGGCCCAGCCCCAGGGCCTGC
Restriction Sites Please inquire |
ACCN NM_016574
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016574.2, NP_057658.2
RefSeq Size 2556 bp
RefSeq ORF 1245 bp
Locus ID 1813
UniProt ID P14416
Cytogenetics 11q23.2
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Gap junction, Neuroactive ligand-receptor interaction
Summary This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) lacks an internal exon, as compared to variant 1, but maintains the same reading frame. It encodes an isoform (short), which is 29 aa shorter than isoform long.
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Documents
Resources
"NM_016574" in other vectors (6)
SKU Description Size Price
RC213065 DRD2 (Myc-DDK-tagged)-Human dopamine receptor D2 (DRD2), transcript variant 2 10 ug
$289.00 $457.00
RC213065L1 Lenti ORF clone of Human dopamine receptor D2 (DRD2), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC213065L2 Lenti ORF clone of Human dopamine receptor D2 (DRD2), transcript variant 2, mGFP tagged 10 ug
$757.00
RC213065L3 Lenti ORF clone of Human dopamine receptor D2 (DRD2), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC213065L4 Lenti ORF clone of Human dopamine receptor D2 (DRD2), transcript variant 2, mGFP tagged 10 ug
$757.00
RG213065 DRD2 (tGFP-tagged) - Human dopamine receptor D2 (DRD2), transcript variant 2 10 ug
$489.00 $657.00

file-alt Citations

γ-Aminobutyric acid type B (GABAB) receptor expression is needed for inhibition of N-type (Cav2.2) calcium channels by analgesic α-conotoxins
Cuny, de Faoite, Huynh et al
J Biol Chem (2012) 287 (28), 23948-57 DOI: 10.1074/jbc.M112.342998
AI Summary: In this study, OriGene products—specifically, GABA(B) receptor subunit R1 and R2 siRNAs—were used to transiently knock down GABA(B) receptor expression in rat dorsal root ganglion neurons, enabling the authors to demonstrate that functional GABA(B) receptors are required for α-conotoxin-mediated inhibition of N-type calcium channels.
Showing 1-1 of 1 papers.
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