NACA2 (NM_199290) Human Untagged Clone

SKU
SC307923
NACA2 (untagged)-Human nascent polypeptide-associated complex alpha subunit 2 (NACA2)
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NACA2
Synonyms ANAC; NACAL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC307923 representing NM_199290.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCCGGGCGAAGCCACAGAAACCGTCCCTGCTACAGAGCAGGAGTTGCCGCAGTCCCAGGCTGAGACA
GGGTCTGGAACAGCATCTGATAGTGGTGAATCAGTACCAGGGATTGAAGAACAGGATTCCACCCAGACC
ACCACACAAAAAGCCTGGCTGGTGGCAGCAGCTGAAATTGATGAAGAACCAGTCGGTAAAGCAAAACAG
AGTCGGAGTGAAAAGAGGGCACGGAAGGCTATGTCCAAACTGGGTCTTCTACAGGTTACAGGAGTTACT
AGAGTCACTATCTGGAAATCTAAGAATATCCTCTTTGTCATCACAAAACTGGACGTCTACAAGAGCCCT
GCTTCGGATGCCTACATAGTTTTTGGGGAAGCCAAGATCCAAGATTTATCTCAGCAAGCACAACTAGCA
GCTGCGGAGAAATTCAGAGTTCAAGGTGAAGCTGTCGGAAACATTCAAGAAAACACACAGACTCCAACT
GTACAAGAGGAGAGTGAAGAGGAAGAGGTCGATGAAACAGGTGTAGAAGTTAAAGACGTGAAATTGGTC
ATGTCACAAGCAAATGTGTCGAGAGCAAAGGCAGTCCGAGCTCTGAAGAACAACAGTAATGATATTGTA
AATGCGATTATGGAATTAACAGTGTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_199290
Insert Size 648 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199290.3
RefSeq Size 770 bp
RefSeq ORF 648 bp
Locus ID 342538
UniProt ID Q9H009
Cytogenetics 17q23.2
MW 23.2 kDa
Summary Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_199290" in other vectors (4)
SKU Description Size Price
RC209370 NACA2 (Myc-DDK-tagged)-Human nascent polypeptide-associated complex alpha subunit 2 (NACA2) 10 ug
$300.00
RC209370L3 Lenti ORF clone of Human nascent polypeptide-associated complex alpha subunit 2 (NACA2), Myc-DDK-tagged 10 ug
$600.00
RC209370L4 Lenti ORF clone of Human nascent polypeptide-associated complex alpha subunit 2 (NACA2), mGFP tagged 10 ug
$600.00
RG209370 NACA2 (tGFP-tagged) - Human nascent polypeptide-associated complex alpha subunit 2 (NACA2) 10 ug
$489.00 $500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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