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PKIB (NM_181794) Human Untagged Clone

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SKU
SC307370
PKIB (untagged)-Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 2
  $165.00
3 Weeks*
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Other products for "PKIB or NM_181794"

Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PKIB
Synonyms PRKACN2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC307370 representing NM_181794.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAGGACAGATTCATCAAAAATGACTGACGTGGAGTCTGGGGTCGCCAATTTTGCATCTTCAGCAAGG
GCAGGCCGCCGGAATGCCTTACCAGACATCCAGAGTTCAGCTGCCACAGACGGAACCTCAGATTTGCCC
CTCAAACTGGAGGCTCTCTCCGTGAAGGAAGATGCAAAAGAGAAAGATGAAAAAACAACACAAGACCAA
TTGGAAAAGCCTCAAAATGAAGAAAAATGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_181794
Insert Size 237 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181794.2
RefSeq Size 1892 bp
RefSeq ORF 237 bp
Locus ID 5570
UniProt ID Q9C010
Cytogenetics 6q22.31
Protein Families Druggable Genome
MW 8.5 kDa
Summary This gene encodes a member of the cAMP-dependent protein kinase inhibitor family. The encoded protein may play a role in the protein kinase A (PKA) pathway by interacting with the catalytic subunit of PKA, and overexpression of this gene may play a role in prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Jul 2012
Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, 3 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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"NM_181794" in other vectors (4)
SKU Description Size Price
RC216291 PKIB (Myc-DDK-tagged)-Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 2 10 ug
$150.00
RC216291L3 Lenti ORF clone of Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC216291L4 Lenti ORF clone of Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 2, mGFP tagged 10 ug
$450.00
RG216291 PKIB (tGFP-tagged) - Human protein kinase (cAMP-dependent, catalytic) inhibitor beta (PKIB), transcript variant 2 10 ug
$350.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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