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BDNF (NM_170731) Human Untagged Clone

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SKU
SC306690
BDNF (untagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 3
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol BDNF
Synonyms ANON2; BULN2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_170731 edited
AATGTTCCACCAGGTGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTATTTCATA
CTTTGGTTGCATGAAGGCTGCCCCCATGAAAGAAGCAAACATCCGAGGACAAGGTGGCTT
GGCCTACCCAGGTGTGCGGACCCATGGGACTCTGGAGAGCGTGAATGGGCCCAAGGCAGG
TTCAAGAGGCTTGACATCATTGGCTGACACTTTCGAACACGTGATAGAAGAGCTGTTGGA
TGAGGACCAGAAAGTTCGGCCCAATGAAGAAAACAATAAGGACGCAGACTTGTACACGTC
CAGGGTGATGCTCAGTAGTCAAGTGCCTTTGGAGCCTCCTCTTCTCTTTCTGCTGGAGGA
ATACAAAAATTACCTAGATGCTGCAAACATGTCCATGAGGGTCCGGCGCCACTCTGACCC
TGCCCGCCGAGGGGAGCTGAGCGTGTGTGACAGTATTAGTGAGTGGGTAACGGCGGCAGA
CAAAAAGACTGCAGTGGACATGTCGGGCGGGACGGTCACAGTCCTTGAAAAGGTCCCTGT
ATCAAAAGGCCAACTGAAGCAATACTTCTACGAGACCAAGTGCAATCCCATGGGTTACAC
AAAAGAAGGCTGCAGGGGCATAGACAAAAGGCATTGGAACTCCCAGTGCCGAACTACCCA
GTCGTACGTGCGGGCCCTTACCATGGATAGCAAAAAGAGAATTGGCTGGCGATTCATAAG
GATAGACACTTCTTGTGTATGTACATTGACCATTAAAAGGGGAAGATAGTGGATTTATGT
TGTATAGATTAGATTATATTGAGACAAAAATTATCTATTTGTATATATACATAACAGGGT
AAATTATTCAGTTAAGAAAAAAATAATTTTATGAACTGCATGTATAAATGAAGTTTATAC
AGTACAGTGGTTCTACAATCTATTTATTGGACATGTCCATGACCAGAAGGGAAACAGTCA
TTTGCGCACAACTTAAAAAGTCTGCATTACATTCCTTGATAATGTTGTGGTTTGTTGCCG
TTGCCAAGAACTGAAAACATAAAAAGTTAAAAAAAATAATAAATTGCATGCTGCTTTAAT
TGTGAATTGAAAAAAAAAAAAAAAAAACTCGAC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_170731 unedited
GTTCACAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGAATGTTCCA
CCAGTGAGAAGAGTGATGACCATCCTTTTCCTTACTATGGTTATTTCATACTTTGGTTGC
ATGAAGGCTGCCCCCATGAAAGAAGCAAACATCCGAGGACAAGGTGGCTTGGCCTACCCA
GGTGTGCGGACCCATGGGACTCTGGAGAGCGTGAATGGGCCCAAGGCAGGTTCAAGAGGC
TTGACATCATTGGCTGACACTTTCGAACACGTGATAGAAGAGCTGTTGGATGAGGACCAG
AAAGTTCGGCCCAATGAAGAAAACAATAAGGACGCAGACTTGTACACGTCCAGGGTGATG
CTCAGTAGTCAAGTGCCTTTGGAGCCTCCTCTTCTCTTTCTGCTGGAGGAATACAAAAAT
TACCTAGATGCTGCAAACATGTCCATGAGGGTCCGGCGCCACTCTGACCCTGCCCGCCGA
GGGGAGCTGAGCGTGTGTGACAGTATTAGTGAGTGGGTAACGGCGGCAGACAAAAAGACT
GCAGTGGACATGTCGGGCGGGACGGTCACAGTCCTTGAAAAGGTCCCTGTATCAAAAGGC
CAACTGAAGCAATACTTCTACGAGACCAAGTGCAATCCCATGGGTTACACAAAAGAANGC
TGCAGGGGCATAGACAAAAGGCATTGGAACTCCCAGTGCCGAACTACCCAGTCGTACGTG
CGGGCCCTTACCATGGATAGCANNAAGAAATTGGCTGGCGATTCATAANGATAGACACTN
TCTTGTGTATGTACATTGACCATTAAAAGGGG
3' Read Nucleotide Sequence
>Forward primer walk for NM_170731 unedited
GCGGCATGTCTTGACNTGTNGNNANNCCCTGTACTGTNATAAACTTCATTTATACATGCA
GTTCATAAAATTATTTTTTTCTTAACTGAATAATTTACCCTGTTATGTATATATACAAAT
AGATAATTTTTGTCTCAATATAATCTAATCTATACAACATAAATCCACTATCTTCCCCTT
TTAATGGTCAATGTACATACACAAGAAGTGTCTATCCTTATGAATCGCCAGCCAATTCTC
TTTTTGCTATCCATGGTAAGGGCCCGCACGTACGACTGGGTAGTTCGGCACTGGGAGTTC
CAATGCCTTTTGTCTATGCCCCTGCAGCCTTCTTTTGTGTAACCCATGGGATTGCACTTG
GTCTCGTAGAAGTATTGCTTCAGTTGGCCTTTTGATACAGGGACCTTTTCAAGGACTGTG
ACCGTCCCGCCCGACATGTCCACTGCAGTCTTTTTGTCTGCCGCCGTTACCCACTCACTA
ATACTGTCACACACGCTCAGCTCCCCTCGGCGGGCAGGGTCAAAGTGGCGCCGGACCCTC
AAGGACATGTTTGCAGCATCTAAGTAATTTTTGAATTCCCCAACCAAAAGAAAAAAAGAA
GCTCCAAAGGCACTTGACTACTGGACCATCAACCCTGGACGTGGACAAGTCCAGCCCTCC
CTATTGTTTAAATAAATGGAACCCAAAATAATAGGAACCAACAAACAAACCCTACAAACA
TCCCCCCCTCCAAAAACAGTCAAGACCAATAAAGTAAAGAACACATAAAACCATGCCCTG
GGGGACAAATACAAACAAACCAAGAATCACAAGGGGACCCCCACACCTGCGGGTAGAGCA
AGACGCACCCGAGCCCCCGGAATAGTAAGAATTTTATAAAGGAGGGAAAAGATAGAAAAC
GGCCAAGCTAAAGAAGATAACGAATGACGAGCCAGAAAGACCGAACGCCGTT
Restriction Sites Please inquire |
ACCN NM_170731
Insert Size 1200 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_170731.3, NP_733927.1
RefSeq Size 4044 bp
RefSeq ORF 768 bp
Locus ID 627
UniProt ID P23560
Cytogenetics 11p14.1
Protein Families Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Transmembrane
Protein Pathways Huntington's disease, MAPK signaling pathway, Neurotrophin signaling pathway
Summary This gene encodes a member of the nerve growth factor family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. Binding of this protein to its cognate receptor promotes neuronal survival in the adult brain. Expression of this gene is reduced in Alzheimer's, Parkinson's, and Huntington's disease patients. This gene may play a role in the regulation of the stress response and in the biology of mood disorders. provided by RefSeq, Nov 2015
Transcript Variant: This variant (3), also known as I, BDNF1, or 1-5, represents use of an alternate promoter and differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (b) contains a longer N-terminus compared to isoform a. This isoform (b) may undergo proteolytic processing similar to isoform a.
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Documents
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"NM_170731" in other vectors (6)
SKU Description Size Price
RC217021 BDNF (Myc-DDK-tagged)-Human brain-derived neurotrophic factor (BDNF), transcript variant 3 10 ug
$300.00
RC217021L1 Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC217021L2 Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 3, mGFP tagged 10 ug
$600.00
RC217021L3 Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC217021L4 Lenti ORF clone of Human brain-derived neurotrophic factor (BDNF), transcript variant 3, mGFP tagged 10 ug
$600.00
RG217021 BDNF (tGFP-tagged) - Human brain-derived neurotrophic factor (BDNF), transcript variant 3 10 ug
$489.00 $500.00

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