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PANK1 (NM_148978) Human Untagged Clone

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SKU
SC306350
PANK1 (untagged)-Human pantothenate kinase 1 (PANK1), transcript variant beta
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "PANK1 or NM_148978"

Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PANK1
Synonyms PANK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_148978
Insert Size 1122 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_148978.2
RefSeq Size 6198 bp
RefSeq ORF 1122 bp
Locus ID 53354
UniProt ID Q8TE04
Cytogenetics 10q23.31
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Pantothenate and CoA biosynthesis
MW 41.7 kDa
Summary This gene encodes a member of the pantothenate kinase family. Pantothenate kinases are key regulatory enzymes in the biosynthesis of coenzyme A (CoA). The encoded protein catalyzes the first and rate-limiting enzymatic reaction in CoA biosynthesis and is regulated by CoA through feedback inhibition. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene and an intronic miRNA on the same strand are co-regulated by the tumor suppressor p53 (see PMID 20833636). provided by RefSeq, Apr 2011
Transcript Variant: This variant (beta) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant alpha. The encoded isoform (beta) has a distinct N-terminus and is shorter than isoform alpha. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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"NM_148978" in other vectors (4)
SKU Description Size Price
RC212009 PANK1 (Myc-DDK-tagged)-Human pantothenate kinase 1 (PANK1), transcript variant beta 10 ug
$457.00
RC212009L3 Lenti-ORF clone of PANK1 (Myc-DDK-tagged)-Human pantothenate kinase 1 (PANK1), transcript variant beta 10 ug
$757.00
RC212009L4 Lenti-ORF clone of PANK1 (mGFP-tagged)-Human pantothenate kinase 1 (PANK1), transcript variant beta 10 ug
$757.00
RG212009 PANK1 (tGFP-tagged) - Human pantothenate kinase 1 (PANK1), transcript variant beta 10 ug
$657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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