TUT1 (NM_022830) Human Untagged Clone

SKU SC305056

TUT1 (untagged)-Human terminal uridylyl transferase 1, U6 snRNA-specific (TUT1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TUT1
• Synonyms: PAPD2; RBM21; STARPAP; TENT1; TUTase; URLC6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC305056 representing NM_022830.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGTCACTTCCTATCGGATCGGCGGAAGTTGCTTCCGAGCGCGTTGAGCTTTGGCGAAGTGGGTTCCGG; TGGTGGCAGAGGTGCTTGTGTTTTTGTCGGTACAGGAGAGTCGCTATGGCGGCGGTGGATTCGGATGTC; GAATCGCTGCCGCGTGGGGGGTTCCGCTGCTGCCTCTGCCACGTTACTACAGCCAACCGACCCAGCCTT; GATGCCCACTTGGGAGGCAGAAAGCACCGGCACCTGGTAGAACTACGAGCTGCGAGAAAGGCCCAGGGA; CTTCGAAGTGTGTTTGTCAGTGGCTTTCCCAGGGATGTGGATTCTGCTCAGCTCTCTGAGTACTTCCTA; GCATTTGGACCTGTGGCCAGTGTTGTCATGGACAAGGACAAGGGAGTGTTTGCCATTGTGGAGATGGGG; GACGTGGGTGCTCGAGAGGCTGTCTTGTCACAGTCCCAGCACAGCCTGGGAGGACATCGCCTGCGTGTC; CGCCCACGGGAGCAGAAGGAGTTCCAGAGCCCGGCCTCCAAATCCCCCAAAGGAGCGGCCCCCGACAGT; CACCAGCTGGCCAAAGCGCTAGCTGAGGCTGCAGACGTGGGGGCACAAATGATAAAGCTTGTGGGGCTG; AGGGAGTTGTCCGAGGCCGAGCGGCAGCTTCGCAGCCTAGTGGTGGCCCTGATGCAGGAGGTCTTCACA; GAGTTCTTCCCTGGCTGTGTGGTCCACCCTTTTGGCTCTTCCATAAATAGCTTCGATGTCCATGGCTGT; GATCTTGACCTCTTCTTGGATCTGGGTGACTTGGAAGAGCCCCAGCCAGTCCCAAAGGCTCCAGAATCT; CCATCGCTGGACTCGGCCCTGGCTTCCCCACTGGACCCTCAAGCCCTGGCCTGCACCCCAGCTTCCCCT; CCAGATTCACAACCTCCTGCTTCTCCCCAGGATTCTGAAGCCCTGGACTTTGAAACCCCTTCCTCCTCC; CTGGCGCCCCAAACTCCGGACTCTGCCTTGGCCTCCGAGACCCTTGCTTCTCCCCAGTCTCTGCCTCCA; GCTTCACCACTGCTAGAGGACAGGGAAGAGGGGGACCTGGGGAAGGCCTCGGAACTAGCAGAGACCCCA; AAGGAGGAGAAAGCAGAGGGGGCAGCAATGCTGGAGCTGGTGGGATCCATTCTCCGGGGCTGTGTCCCT; GGGGTGTATCGAGTCCAAACTGTGCCCTCTGCCCGGCGCCCTGTGGTCAAGTTCTGTCATCGGCCTTCA; GGTCTCCACGGTGATGTCTCCCTCAGTAACCGGCTGGCCCTGCATAACTCCCGTTTCCTGAGTCTCTGC; TCTGAGCTGGATGGTCGAGTCCGGCCCCTCGTGTACACCCTCCGCTGCTGGGCTCAGGGTCGGGGGCTG; TCAGGGAGTGGCCCCCTTCTCAGTAACTACGCCCTGACCTTGCTGGTGATCTATTTTCTTCAGACCAGG; GACCCTCCTGTGTTGCCCACTGTGTCCCAGCTCACCCAGAAAGCAGGAGAGGGGGAACAGGTGGAAGTC; GATGGCTGGGACTGCAGTTTCCCCAGGGATGCCTCAAGACTGGAGCCCAGCATAAATGTGGAGCCCCTC; AGTTCCCTGCTAGCCCAGTTCTTCTCCTGTGTATCTTGTTGGGATCTTCGTGGCTCCCTGCTGTCCCTG; CGGGAGGGTCAGGCACTGCCTGTGGCAGGGGGCCTGCCTTCTAATCTCTGGGAGGGTCTGCGCCTTGGC; CCCCTGAATCTCCAGGACCCTTTTGACCTGAGTCACAATGTCGCAGCCAATGTGACCAGCCGGGTGGCT; GGGCGCCTACAGAACTGCTGCCGAGCAGCAGCCAATTACTGCCGAAGCCTCCAGTACCAGCGCCGTTCC; TCCCGGGGTCGGGACTGGGGGCTGCTCCCTCTTCTGCAGCCCAGCTCCCCCAGCTCCCTGCTCTCTGCT; ACGCCGATCCCTTTACCCCTTGCACCCTTCACCCAGCTCACTGCTGCCCTGGTGCAGGTATTCAGGGAA; GCACTGGGGTGCCATATAGAACAGGCAACCAAGAGAACGCGGTCAGAAGGAGGTGGAACTGGGGAGTCC; TCTCAGGGAGGGACAAGCAAAAGACTCAAAGTAGATGGACAGAAAAACTGCTGTGAGGAGGGGAAAGAG; GAGCAGCAGGGATGTGCAGGGGACGGTGGGGAAGACAGGGTAGAAGAGATGGTTATAGAGGTTGGAGAG; ATGGTGCAGGACTGGGCCATGCAGAGCCCTGGGCAGCCAGGGGACCTGCCCCTGACCACTGGAAAGCAT; GGAGCCCCTGGAGAAGAGGGGCAGCCCAGCCACGCAGCCCTGGCAGAGCGGGGGCCCAAGGGACATGAG; GCAGCCCAAGAATGGTCTCAGGGTGAGGCAGGGAAGGGGGCATCCCTGCCCTCCTCAGCGAGCTGGCGC; TGTGCCTTGTGGCACCGAGTGTGGCAAGGGCGGCGGCGAGCCCGTAGACGCTTGCAGCAGCAAACCAAG; GAGGGAGCTGGAGGTGGCGCTGGCACAAGAGCAGGGTGGCTGGCGACTGAGGCTCAGGTCACCCAGGAG; CTGAAAGGACTGAGTGGTGGCGAAGAGAGGCCAGAAACTGAGCCCCTGCTGAGCTTTGTGGCGTCTGTC; TCCCCGGCTGACCGAATGCTCACTGTGACCCCGCTCCAGGATCCCCAAGGCCTGTTCCCTGATCTCCAT; CATTTCTTACAGGTTTTCCTCCCTCAAGCAATTCGACATCTCAAGTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_022830
• Insert Size: 2739 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022830.2
• RefSeq Size: 2844 bp
• RefSeq ORF: 2739 bp
• Locus ID: 64852
• UniProt ID: Q9H6E5
• Cytogenetics: 11q12.3
• MW: 98.4 kDa
• Summary: This gene encodes a nucleotidyl transferase that functions as both a terminal uridylyltransferase and a nuclear poly(A) polymerase. The encoded enzyme specifically adds and removes nucleotides from the 3' end of small nuclear RNAs and select mRNAs and may function in controlling gene expression and cell proliferation.[provided by RefSeq, Apr 2009]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to start at a downstream in-frame start codon that is supported by transcript and conservation data.