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HYDIN (NM_017558) Human Untagged Clone

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SKU
SC304483
HYDIN (untagged)-Human hydrocephalus inducing homolog (mouse) (HYDIN), transcript variant 2
  $886.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol HYDIN
Synonyms CILD5; HYDIN1; HYDIN2; PPP1R31
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC304483 representing NM_017558.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGACAAGTAGAAGACTTGAGGAGTCCATGGGGGCTGTTCAGATGGGATTGGTCAATATGTTCAAAGGA
TTTCAAAGCAAGGTTTTGCCACCCCTGAGTCCAAAGGTGGTTACAGAAGAAGAAGTAAACCGAATGCTT
ACACCCTCAGAGTTCCTGAAGGAAATGTCCCTGACCACCGAGCAGAGACTGGCAAAAACACGTTTGATG
TGCCGACCACAGATCATCGAACTCTTAGATATGGGGGAAACAACACATCAGAAGTTTTCAGGAATTGAC
CTGGATCAGGCATTATTCCAGCCCTTTCCATCAGAAATTATATTTCAGAACTACACTCCCTGTGAAGTC
TATGAAGTTCCACTGATTTTGAGGAACAATGACAAAATTCCAAGGTTGGTGAAAGTTGTGGAAGAAAGT
TCGCCTTACTTTAAAGTAATCAGCCCCAAAGATATTGGCCACAAAGTGGCTCCTGGAGTGCCTTCCATA
TTCCGAATCCTCTTTACTCCAGAGGAGAACAAGGATTACGCCCATACGTTGACCTGTGTTACTGAAAGA
GAAAAGTTTATTGTACCCATCAAAGCTAGAGGGGCACGAGCCATTCTCGATTTTCCTGACAAGCTGAAT
TTTTCCACTTGTCCTGTCAAATACAGCACCCAGAAGATTCTGCTGGTACGAAACATTGGCAACAAAAAT
GCTGTATTTCACATCAAAACTTGTAGGCCTTTCTCTATAGAACCAGCTATTGGAACTCTTAATGTGGGA
GAGTCCATGCAACTGGAAGTGGAGTTTGAGCCACAGAGTGTGGGCGATCACAGTGGAAGACTTATCGTG
TGTTATGACACAGGTGAAAAGGTGTTTGTATCTCTCTATGGAGCTGCCATAGACATGAATATAAGGCTG
GATAAGAATTCCTTGACCATCGAGAAAACCTACATATCTCTGGCCAATCAGCGAACTATAACCATTCAC
AATCGCAGTAATATCATTGCCCATTTCCTGTGGAAGGTATTTGCTACCCAGCAAGAAGAGGACAGAGAA
AAATATAGGGCCTGTGATGATCTGATCAAAGAGGAGAAGGATGAGACTGATGAGTTTTTTGAAGAGTGC
ATTACTGATCCTTTACTCCGAGAACATCTTTCTGTTCTGTCCCGAACCTTTGCGAATCAGAGGAGGCTG
GTGCAGGGAGACAGCAAGCTGTTCTTCAATAACGTTTTCACTGTGGAGCCCCTGGAAGGTGATGTCTGG
CCCAACTCATCAGCTGAAATCACCGTGTACTTTAACCCACTAGAAGCCAAGCTCTATCAACAGACCATT
TACTGCGACATTTTAGGCCGAGAAATCCGTCTGCCCCTCCGAATCAAAGGGGAAGGCATGGGACCTAAG
ATTCACTTCAACTTTGAATTGCTGGATATTGGGAAAGTTTTCACTGGATCTGCACATTGTTATGAGGCG
ATACTGTACAACAAAGGCAGCATCGATGCTCTCTTCAACATGACCCCTCCAACTTCAGCTTTGGGGGCC
TGCTTTGTTTTCAGTCCCAAGGAAGGCATCATTGAACCAAGTGGAGTCCAAGCTATCCAGATCTCCTTC
AGCTCTACCATCCTGGGAAACTTTGAAGAAGAGTTCCTGGTCAATGTCAATGGGTCACCTGAGCCTGTG
AAACTGACCATTAGAGGCTGTGTCATTGGACCTACCTTCCATTTTAATGTTCCAGCTCTGCACTTTGGT
GATGTTTCCTTTGGGTTTCCTCATACCTTGATATGTTCCCTCAATAATACCTCTTTGATCCCCATGACT
TACAAACTGCGTATCCCTGGGGATGGCCTTGGCCATAAAAGCATTTCATATTGTGAGCAGCATGTGGAC
TACAAAAGACCATCTTGGACCAAGGAAGAAATATCCTCAATGAAACCAAAAGAATTCACCATCTCTCCT
GACTGTGGCACCATTCGCCCCCAGGGATTTGCTGCTATCAGGGTGACATTATGCTCCAACACTGTGCAG
AAATACGAGCTGGCACTCGTGGTGGACGTGGAGGGCATCGGAGAAGAGGTGCTGGCGCTCTTAATTACA
GCAAGGTGTGTTGTACCTGCCCTCCACCTGGTCAATACAGAGGTGGACTTTGGGCACTGCTTCCTGAAG
TACCCGTATGAGAAAACACTCCAGCTTGCCAATCAAGATGACCTCCCAGGATTCTATGAGGTCCAGCCT
CAGGTGTGTGAGGAGGTGCCTACTGTGCTGTTTTCCAGCCCCACCCCCAGCGGGGTCATCTCCCCAAGC
AGCACCATCCACATACCACTGGTCCTGGAGACCCAGGTCACTGGAGAACACAGATCCACGGTTTACATC
TCAATCTTTGGGAGCCAGGACCCCCCTTTGGTATGTCACTTAAAGAGCGCTGGAGAAGGCCCAGTTATC
TACGTCCATCCCAATCAAGTGGACTTCGGGAATATCTACGTCCTAAAAGACTCTTCCAGGATTCTCAAC
CTATGCAACCAGTCCTTCATTCCCGCATTTTTCCAGGCACACATGGCACACAAAAAATCCCTTTGGACG
ATTGAACCCAATGAAGGCATGGTTCCTCCAGAAACTGATGTTCAACTGGCACTGACCGCCAACCTGAAT
GACACACTGACATTCAAGGACTGTGTTATTTTGGACATTGAAAATAGCAGTACCTATCGGATTCCTGTT
CAGGCTTCCGGAACTGGTTCCACTATTGTTTCAGATAAGCCCTTTGCTCCAGAACTCAATTTGGGGGCA
CATTTTAGCCTGGATACCCACTATTACCACTTTAAGTTGATCAACAAGGGACGTCGGATCCAACAGTTG
TTCTGGATGAATGATAGCTTCCGACCCCAGGCCAAGCTGAGTAAGAAGGGCCGGGTTAAGAAGGGACAT
GCTCATGTCCAACCCCAGCCCAGTGGCTCTCAGGAGCCCAGGGATCCACAGAGCCCCGTGTTTCATCTC
CACCCCGCCAGCATGGAGCTGTACCCAGGCCAGGCAATTGATGTGATACTCGAAGGCTATTCTGCTACT
CCCAGGGTAAGGGGATAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_017558
Insert Size 3054 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017558.2
RefSeq Size 3718 bp
RefSeq ORF 3054 bp
Locus ID 54768
UniProt ID Q4G0P3
Cytogenetics 16q22.2
MW 114.2 kDa
Summary This gene encodes a protein that may be involved in cilia motility. Mutations in this gene cause of autosomal recessive primary ciliary dyskinesia-5, a disorder characterized by the accumulation of cerebrospinal fluid within the ventricles of the brain. A duplicate copy of this gene has been found in humans on chromosome 1. provided by RefSeq, Jan 2013
Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (b) has a distinct C-terminus and is shorter than isoform a.
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Documents
Resources
"NM_017558" in other vectors (4)
SKU Description Size Price
RC221325 HYDIN (Myc-DDK-tagged)-Human hydrocephalus inducing homolog (mouse) (HYDIN), transcript variant 2 10 ug
$885.00
RC221325L3 Lenti ORF clone of Human hydrocephalus inducing homolog (mouse) (HYDIN), transcript variant 2, Myc-DDK-tagged 10 ug
$1,185.00
RC221325L4 Lenti ORF clone of Human hydrocephalus inducing homolog (mouse) (HYDIN), transcript variant 2, mGFP tagged 10 ug
$1,185.00
RG221325 HYDIN (tGFP-tagged) - Human hydrocephalus inducing homolog (mouse) (HYDIN), transcript variant 2 10 ug
$1,085.00

file-alt Citations

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