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PACS2 (NM_015197) Human Untagged Clone

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SKU
SC304251
PACS2 (untagged)-Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 2
  $896.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PACS2
Synonyms EIEE66; PACS-2; PACS1L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC304251 representing NM_015197.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCCGAGCGAGGCCGCCTCGGCCTCCCCGGCGCGCCCGGCGCGCTCAACACGCCCGTGCCCATGAAC
CTGTTCGCCACCTGGGAGGTGGACGGCTCCAGCCCCAGCTGCGTGCCCAGGTTGTGCAGCCTGACTCTG
AAGAAGCTGGTGGTCTTCAAGGAGCTGGAGAAGGAGCTGATCTCCGTGGTGATCGCTGTCAAGATGCAG
GGCTCCAAACGAATCCTGCGGTCCCATGAGATTGTGCTGCCCCCCAGTGGACAAGTGGAGACAGACCTG
GCCCTGACCTTCTCCTTGCAGTATCCTCACTTCTTGAAGAGGGAAGGCAACAAGCTTCAGATCATGCTG
CAGCGCAGAAAGCGCTACAAGAACAGAACCATCCTGGGCTACAAGACGCTGGCCGCGGGCTCCATCAGC
ATGGCTGAGGTGATGCAACACCCGTCTGAAGGTGGCCAGGTGCTGAGCCTCTGCAGCAGCATCAAGGAG
GCCCCCGTCAAGGCGGCCGAGATCTGGATCGCCTCCCTGTCCAGCCAGCCCATTGACCACGAAGACAGC
ACCATGCAGGCCGGCCCCAAGGCCAAGTCCACGGATAACTACTCCGAGGAGGAGTATGAGAGCTTCTCC
TCCGAGCAGGAGGCCAGTGACGACGCCGTGCAGGGGCAGGACTTGGACGAGGACGACTTTGACGTGGGG
AAGCCGAAGAAGCAGCGGAGATCGATTGTAAGAACGACGTCCATGACCAGGCAACAGAACTTCAAGCAG
AAAGTGGTAGCGCTGCTGCGGAGGTTCAAAGTGTCCGACGAGGTCCTGGACTCGGAGCAGGACCCTGCG
GAGCACATCCCCGAGGCAGAGGAGGACCTGGACCTCCTGTATGACACCCTGGACATGGAGCACCCCAGC
GACAGCGGCCCCGACATGGAGGATGACGACAGCGTCCTCAGCACCCCCAAGCCGAAGCTGCGGCCATAC
TTTGAAGGCCTGTCGCACTCGAGCTCGCAGACGGAGATTGGGAGCATCCACAGCGCCCGCAGCCACAAG
GAGCCCCCAAGCCCGGCTGACGTGCCCGAGAAGACGCGGTCCCTGGGAGGCAGGCAGCCGAGCGACAGT
GTCTCTGACACGGTGGCCCTCGGTGTGCCAGGCCCGAGGGAGCACCCTGGACAGCCTGAGGACAGCCCC
GAGGCTGAGGCCTCCACCCTGGATGTGTTCACGGAGAGGCTGCCGCCCAGCGGGAGGATCACCAAGACA
GAGTCCCTTGTCATCCCCTCCACCAGGTCCGAGGGGAAGCAGGCTGGCCGACGGGGCCGGAGCACATCC
TTGAAGGAGCGGCAGGCAGCACGGCCCCAGAATGAGCGGGCCAACAGCCTGGACAACGAGCGCTGCCCG
GACGCCCGGAGCCAGCTACAGATCCCCAGGAAGACTGTGTATGACCAGCTCAACCACATCCTCATCTCC
GATGACCAGCTTCCCGAAAACATCATCCTTGTCAACACCTCGGACTGGCAGGGGCAGTTCCTCTCCGAC
GTCCTGCAGAGGCACACGCTCCCCGTGGTGTGCACGTGCTCTCCTGCGGACGTCCAGGCGGCCTTCAGC
ACCATCGTCTCACGGATACAGAGATACTGCAACTGCAATTCCCAGCCCCCGACCCCCGTGAAGATCGCC
GTGGCGGGAGCGCAGCATTACCTCAGTGCCATCCTGCGGCTCTTTGTGGAGCAGCTGTCCCACAAGACA
CCCGACTGGCTCGGCTACATGCGCTTCCTGGTCATCCCACTGGGCTCCCACCCCGTGGCCAGGTACCTA
GGCTCCGTGGACTACCGCTACAACAACTTCTTCCAGGACCTGGCCTGGAGAGACCTGTTCAACAAGCTG
GAGGCCCAGAGTGCGGTACAGGACACGCCAGACATTGTGTCACGCATCACGCAGTACATCGCAGGGGCC
AACTGTGCCCACCAGCTCCCCATCGCAGAGGCCATGCTGACCTACAAGCAGAAGAGCCCTGACGAAGAG
TCCTCCCAAAAGTTCATTCCCTTTGTCGGGGTTGTGAAGGTTGGAATTGTGGAGCCATCCTCGGCCACA
TCAGGCGACTCGGACGACGCGGCCCCCTCGGGCTCTGGCACGCTCTCCTCCACCCCGCCGTCCGCATCT
CCTGCGGCCAAGGAGGCCTCACCCACCCCGCCCTCCTCCCCGTCGGTGAGCGGAGGCCTGTCCTCCCCC
AGCCAGGGTGTCGGCGCCGAGCTGATGGGGCTGCAGGTGGACTACTGGACGGCAGCACAGCCTGCGGAC
AGGAAGAGGGACGCCGAGAAGAAGGACCTGCCTGTCACCAAAAACACGCTCAAGTGCACTTTCCGGTCC
CTCCAGGTCAGCAGGCTGCCCAGCAGCGGCGAGGCTGCAGCCACGCCCACCATGTCCATGACCGTGGTC
ACCAAGGAGAAGAACAAGAAGGTGATGTTTCTGCCCAAGAAAGCGAAGGACAAGGACGTGGAGTCTAAG
AGCCAGTGCATTGAGGGCATCAGCCGGCTCATCTGCACTGCCAGGCAGCAGCAGAACATGCTGCGGGTC
CTCATCGACGGCGTGGAGTGCAGCGACGTCAAGTTCTTCCAGCTGGCCGCGCAGTGGTCCTCGCACGTG
AAGCACTTCCCCATCTGCATCTTCGGACACTCCAAGGCCACCTTCTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_015197
Insert Size 2670 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015197.3
RefSeq Size 6667 bp
RefSeq ORF 2670 bp
Locus ID 23241
UniProt ID Q86VP3
Cytogenetics 14q32.33
MW 97.7 kDa
Summary Multifunctional sorting protein that controls the endoplasmic reticulum (ER)-mitochondria communication, including the apposition of mitochondria with the ER and ER homeostasis. In addition, in response to apoptotic inducer, translocates BIB to mitochondria, which initiates a sequence of events including the formation of mitochondrial truncated BID, the release of cytochrome c, the activation of caspase-3 thereby causing cell death. May also be involved in ion channel trafficking, directing acidic cluster-containing ion channels to distinct subcellular compartments.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Resources
"NM_015197" in other vectors (6)
SKU Description Size Price
RC209593 PACS2 (Myc-DDK-tagged)-Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 2 10 ug
$589.00 $814.00
RC209593L1 Lenti-ORF clone of PACS2 (Myc-DDK-tagged)-Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 2 10 ug
$1,114.00
RC209593L2 Lenti-ORF clone of PACS2 (mGFP-tagged)-Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 2 10 ug
$1,114.00
RC209593L3 Lenti-ORF clone of PACS2 (Myc-DDK-tagged)-Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 2 10 ug
$1,114.00
RC209593L4 Lenti-ORF clone of PACS2 (mGFP-tagged)-Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 2 10 ug
$1,114.00
RG209593 PACS2 (tGFP-tagged) - Human phosphofurin acidic cluster sorting protein 2 (PACS2), transcript variant 2 10 ug
$1,014.00

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