MESD (NM_015154) Human Untagged Clone

SKU SC304244

MESDC2 (untagged)-Human mesoderm development candidate 2 (MESDC2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MESD
• Synonyms: BOCA; MESDC2; OI20
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015154 edited; GCGCGGCGCAGCGAAAATGGCGGCTTCCAGGTGGGCGCGCAAGGCCGTGGTCCTGCTTTG; TGCCTCTGACCTGCTGCTGCTGCTGCTACTGCTACCACCGCCTGGGTCCTGCGCGGCCGA; AGGCTCGCCCGGGACGCCCGACGAGTCTACCCCACCTCCCCGGAAGAAGAAGAAGGATAT; TCGCGATTACAATGATGCAGACATGGCGCGTCTTCTGGAGCAATGGGAGAAAGATGATGA; CATTGAAGAAGGAGATCTTCCAGAGCACAAGAGACCTTCAGCACCTGTCGACTTCTCAAA; GATAGACCCAAGCAAGCCTGAAAGCATATTGAAAATGACGAAAAAAGGGAAGACTCTCAT; GATGTTTGTCACTGTATCAGGAAGCCCTACTGAGAAGGAGACAGAGGAAATTACGAGCCT; CTGGCAGGGCAGCCTTTTCAATGCCAACTATGACGTCCAGAGGTTCATTGTGGGATCAGA; CCGTGCTATCTTCATGCTTCGCGATGGGAGCTACGCCTGGGAGATCAAGGACTTTTTGGT; CGGTCAAGACAGGTGTGCTGATGTAACTCTGGAGGGCCAGGTGTACCCCGGCAAAGGAGG; AGGAAGCAAAGAGAAAAATAAAACAAAGCAAGACAAGGGCAAAAAAAAGAAGGAAGGAGA; TCTGAAATCTCGGTCTTCCAAGGAAGAAAATCGAGCTGGGAATAAAAGAGAAGACCTGTG; ATGGGGCAGCAGTGACGCGCTGTGGGGGGACAGGTGGACGTGGAGAGCTCTTTGCCCAGC; TCCTGGGGTGGGAGTGGTCTCAGGCAACTGCACACCGGATGACATTCTAGTGTCTTCTAG; AAAGGGTCTGCCACATGACCAGTTTGTGGTCAAAGAATTACTGCTTAATAGGCTTCAAGT; AAGAAGACAGATGTTTTCTAATTAATACTGGACACTGACAAATTCATGTTTACTATAAAA; TCTCCTTACATGGAAATGTGACTGTGTTGCTTTTTCCCATTTACACTTGGTGAGTCATCA; ACTCTACTGAGATTCCACTCCCCTCCAAGCACCTGCTGTGATTGGGTGGCCTGCTCTGAT; CAGATAGCAAATTCTGATCAGAGAAGACTTTAAAACTCTTGACTTAATTGAGTAAACTCT; TCATGCCATATACATCATTTTCATTATGTTAAAGGTAAAATATGCTTTGTGAACTCAGAT; GTCTGTAGCCAGGAAGCCAGGGTGTGTAAATCCAAAATCTATGCAGGAAATGCGGAGAAT; AGAAAATATGTCACTTGAAATCCTAAGTAGTTTTGAATTTCTTTGACTTGAATCTTACTC; ATCAGTAAGAGAACTCTTGGTGTCTGTCAGGTTTTATGTGGTCTGTAAAGTTAGGGGTTC; TGTTTTGTTTCCTTATTTAGGAAAGAGTACTGCTGGTGTCGAGGGGTTATATGTTCCATT; TAATGTGACAGTTTTAAAGGATTTAAGTAGGGAATCAGAGTCCTTTGCAGAGTGTGACAG; ACGACTCAATAACCTCATTTGTTTCTAAACATTTTTCTTTGATAAAGTGCCTAAATCTGT; GCTTTCGTATAGAGTAACATGATGTGCTACTGTTGATGTCTGATTTTGCCGTTCATGTTA; GAGCCTACTGTGAATAAGAGTTAGAACATTTATATACAGATGTCATTTCTAAGAACTAAA; ATTCTTTGGGAAAAACCCTCAATTGTGATTTTAATAAATTAAAAGTAGCACATTAAAAAA; AAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015154 unedited; TTCAGAATTGTATACGATCATATGGGCGGCCGCGCAATCGGCACGAGGCGCGGCGCAGCG; AAAATGGCGGCTTCCAGGTGGGCGCGCAAGGCCGTGGTCCTGCTTTGTGCCTCTGACCTG; CTGCTGCTGCTGCTACTGCTACCACCGCCTGGGTCCTGCGCGGCCGAAGGCTCGCCCGGG; ACGCCCGACGAGTCTACCCCACCTCCCCGGAAGAAGAAGAAGGATATTCGCGATTACAAT; GATGCAGACATGGCGCGTCTTCTGGAGCCATGGGAGAAAGATGATGACATTGCAGAAGGA; GATCTTCCAGAGCACAAGAGACCTTCAGCACCTGTCGACTTCTCAAAGATAGACCCAAGC; AAGCCTGAAAGCATATTGAAAATGACGAAAACTGGGAAGACTCTCATGATGTTTGTCACT; GTATCAGGAAGCCCTACTGAGAAGGAGACAGAGGAAATTACTAGCCTCTGGCAGGGCAGC; CTTTTCAATGCCAACTATGACGTCCAGAGGTTCATTGTGGGATCAGACCGTGCTATCTTC; ATGCTTCGCGATGGGAGCTACGCCTGGGAGAGCAAGGACTTTTTGGTCGGACAAGACAGG; TGTGCTGATGTAACTCTGGAGGGCCAGGTGTACCCCGGCAAAGGAGGAGGAAGCAAAGAG; AAAAATACCACAAAGCANGACAAGGGCTAAACACAGCAGGAAGGAGATCTGACATCTCGG; TCTTCCCAGGAAGAAATCGAGCTGGGAATAAAGAGAGACCTGTGATGGGCCGCAGTGACC; TCTGTGGGGGCACGGTGGACGTGCGAGCTCTATGCCACTCCTGNGGGGGAGTGGCTCAGC; AACTGCCACCGCAGACATCTATGTCTTTACACGGGTCTGCCCAGACCGTTTGTGTCAAGA; TACTGGTAAAGGCTTCAGTAGAGACAGAGTTTCTATT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015154 unedited; ATGGGATGGCAACTTCCATTTCCAGGNANAGCACTGGGGCAGGGTCACAGGGATGCCACC; CGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTAATGGGCTACTTTTAATTTATTAAAATCACAATTG; AGGGTTTTTCCCAAAGAATTTTAGTTCTTAAAAATGACATCTGTATATAAATGTTCTAAC; TCTTATTCACAGTAGGCTCTAACATGAACGGCAAAATCAGACATCAACAGTAGCACATCA; TGTTACTCTATACGAAAGCACAGATTTAGGCACTTTATCAAAGAAAAATGTTTAGAAACA; AATGAGGTTATTGAGTCGTCTGTCACACTCTGCAAAGGACTCTGATTCCCTACTTAAATC; CTTTAAAACTGTCACATTAAATGGAACATATAACCCCTCGACACCAGCAGTACTCTTTCC; TAAATAAGGAAACAAAACAGAACCCCTAACTTTACAGACCACATAAAACCTGACAGACAC; CAAGAGTTCTCTTACTGATGAGTAAGATTCAAGTCAAAGAAATTCAAAACTACTTAGGAT; TTCAAGTGACATATTTTCTATTCTCCGCATTTCCTGCATAGATTTTGGATTTACACACCC; TGGCTTCCTGGCTACAGACATCTGAGTTCACAAAGCATATTTTAACTTTTACATAATGAA; AATGAGGTTTATGGCATGGAAAGTTTACTCAATTTAATCACAGAGTTTAAAGCCTTTTCT; GATTAAAATTGTGTTTCTTATCAGAACAGGGCCCCCATTCACGCAGGTGCTTGGAGGGGG; ATGGGATCCTCATTGAATTGATGACTCACCCAATGTAATGTGGAAAAACACCCCGCTCCC; TTTTTCTGTGTGGGGGATTTTTATGTAAACTGGATTTGTCCGGTGCCCCTTTAT;
• Restriction Sites: NotI-NotI
• ACCN: NM_015154
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015154.1, NP_055969.1
• RefSeq Size: 4243 bp
• RefSeq ORF: 705 bp
• Locus ID: 23184
• UniProt ID: Q14696
• Cytogenetics: 15q25.1
• Summary: Chaperone specifically assisting the folding of beta-propeller/EGF modules within the family of low-density lipoprotein receptors (LDLRs). Acts as a modulator of the Wnt pathway through chaperoning the coreceptors of the canonical Wnt pathway, LRP5 and LRP6, to the plasma membrane. Essential for specification of embryonic polarity and mesoderm induction. Plays an essential role in neuromuscular junction (NMJ) formation by promoting cell-surface expression of LRP4 (By similarity). May regulate phagocytosis of apoptotic retinal pigment epithelium (RPE) cells (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript and encodes the protein.