MYT1L (NM_015025) Human Untagged Clone
Product Data | |
Type | Human Untagged Clone |
---|---|
Target Symbol | MYT1L |
Synonyms | MRD39; myT1-L; NZF1; ZC2H2C2; ZC2HC4B |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
Fully Sequenced ORF
>SC304207 representing NM_015025.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGTGGACACCGAGGAGAAGCGGCATCGCACGCGGTCCAAAGGGGTTCGAGTTCCCGTGGAACCA GCCATACAAGAGCTGTTCAGCTGTCCCACCCCTGGCTGTGACGGCAGTGGTCATGTCAGTGGCAAATAT GCAAGACACAGAAGTGTATATGGTTGTCCCTTGGCGAAAAAAAGAAAAACACAAGATAAACAGCCCCAG GAACCTGCTCCTAAACGAAAGCCATTTGCCGTGAAAGCAGACAGCTCCTCAGTGGATGAGTGTGACGAC AGTGATGGGACTGAGGACATGGATGAGAAGGAGGAGGATGAGGGGGAGGAGTACTCCGAGGACAATGAT GAGCCAGGGGATGAGGACGAGGAGGACGAGGAGGGGGACCGGGAGGAGGAGGAGGAGATCGAGGAGGAG GATGAGGACGATGACGAGGATGGAGAAGATGTGGAGGATGAAGAAGAGGAAGAGGAGGAGGAGGAGGAG GAGGAAGAGGAAGAAGAAAACGAAGACCATCAAATGAATTGTCACAATACTCGAATAATGCAAGACACA GAAAAGGATGATAACAATAATGACGAATATGACAATTACGATGAACTGGTGGCCAAGTCATTGTTAAAC CTCGGCAAAATCGCTGAGGATGCAGCCTACCGGGCCAGGACTGAGTCAGAAATGAACAGCAATACCTCC AATAGTCTGGAAGACGATAGTGACAAAAACGAAAACCTGGGTCGGAAAAGTGAGTTGAGTTTAGACTTA GACAGTGATGTTGTTAGAGAAACAGTGGACTCCCTTAAACTATTAGCCCAAGGACACGGTGTTGTGCTC TCAGAAAACATGAATGACAGAAATTATGCAGACAGCATGTCGCAGCAAGACAGTAGAAATATGAATTAC GTCATGTTGGGGAAGCCCATGAACAACGGACTCATGGAAAAGATGGTGGAGGAGAGCGATGAGGAGGTG TGTCTGAGCAGTCTGGAGTGTTTGAGGAATCAGTGCTTCGACCTGGCCAGGAAGCTCAGTGAGACCAAC CCGCAGGAGAGGAATCCGCAGCAGAACATGAACATCCGTCAGCATGTCCGGCCAGAAGAGGACTTCCCC GGAAGGACGCCGGACAGAAACTACTCGGACATGCTGAACCTCATGCGGCTGGAGGAGCAGTTGAGCCCC CGGTCGAGAGTGTTTGCCAGCTGTGCGAAGGAGGATGGGTGTCATGAGCGGGACGACGATACCACCTCT GTGAACTCGGACAGGTCTGAAGAGGTGTTCGACATGACCAAGGGGAACCTGACCCTGCTGGAGAAAGCC ATCGCTTTGGAAACGGAAAGAGCAAAGGCCATGAGGGAGAAGATGGCCATGGAAGCTGGGAGGAGGGAC AATATGAGGTCATATGAGGACCAGTCTCCGAGACAACTTCCCGGGGAGGACAGAAAGCCTAAATCCAGT GACAGCCATGTCAAAAAGCCATACTATGATCCCTCAAGAACAGAAAAGAAAGAGAGCAAGTGTCCAACC CCCGGGTGTGATGGAACCGGCCACGTAACTGGGCTGTACCCACATCACCGCAGCCTGTCCGGATGCCCG CACAAAGATAGGGTCCCTCCAGAAATCCTTGCCATGCATGAAAGTGTCCTCAAGTGCCCCACTCCGGGC TGCACGGGGCGCGGGCATGTCAACAGCAACAGGAACTCCCACCGAAGCCTCTCCGGATGCCCGATCGCT GCAGCAGAGAAACTGGCCAAGGCACAGGAAAAGCACCAGAGCTGCGACGTGTCCAAGTCCAGCCAGGCC TCGGACCGCGTGCTCAGGCCAATGTGCTTTGTGAAGCAGCTGGAGATTCCTCAGTATGGCTACAGAAAC AATGTCCCCACAACTACGCCGCGTTCCAACCTGGCCAAGGAGCTCGAGAAATATTCCAAGACCTCGTTT GAATACAACAGTTACGACAACCATACTTATGGCAAGCGAGCCATAGCTCCCAAGGTGCAAACCAGGGAT ATATCCCCCAAAGGATATGATGATGCGAAGCGGTACTGCAAGGACCCCAGCCCCAGCAGCAGCAGCACC AGCAGCTACGCGCCCAGCAGCAGCAGCAACCTGAGCTGCGGCGGGGGCAGCAGCGCCAGCAGCACGTGC AGCAAGAGCAGCTTCGACTACACGCACGACATGGAGGCGGCCCACATGGCGGCCACCGCCATCCTCAAC CTGTCCACGCGCTGCCGCGAGATGCCGCAGAACCTGAGCACCAAGCCGCAGGACCTGTGCGCCACGCGG AACCCTGACATGGAGGTGGATGAGAACGGGACCCTGGACCTCAGCATGAACAAGCAGAGGCCGCGGGAC AGCTGCTGCCCCATCCTGACCCCTCTGGAGCCCATGTCCCCCCAGCAGCAGGCAGTGATGAACAACCGG TGTTTCCAGCTGGGCGAGGGCGACTGCTGGGACTTGCCCGTAGACTACACCAAAATGAAACCCCGGAGG ATAGACGAGGACGAGTCCAAAGACATTACTCCAGAAGACTTGGACCCATTCCAGGAGGCTCTAGAAGAA AGACGGTATCCCGGGGAGGTGACCATCCCAAGTCCCAAACCCAAGTACCCTCAGTGCAAGGAGAGCAAA AAGGACTTAATAACTCTGTCTGGCTGCCCCCTGGCGGACAAAAGCATTCGAAGTATGCTGGCCACCAGC TCCCAAGAACTCAAGTGCCCCACGCCTGGCTGTGATGGTTCTGGACATATCACCGGCAATTATGCTTCT CATCGGAGCCTTTCAGGTTGCCCAAGAGCAAAGAAAAGTGGTATCAGGATAGCACAGAGCAAAGAAGAT AAAGAAGATCAAGAACCCATCAGGTGTCCGGTCCCCGGGTGCGACGGCCAGGGCCACATCACTGGGAAG TACGCGTCCCATCGCAGCGCCTCCGGGTGCCCCTTGGCGGCCAAGAGGCAGAAAGACGGGTACCTGAAT GGCTCCCAGTTCTCCTGGAAGTCGGTCAAGACGGAAGGCATGTCCTGCCCCACGCCAGGATGCGACGGC TCAGGCCACGTCAGCGGCAGCTTCCTCACACACCGCAGCTTGTCAGGATGCCCGAGAGCCACGTCAGCG ATGAAGAAGGCAAAGCTTTCTGGAGAGCAGATGCTGACCATCAAACAGCGGGCCAGCAACGGTATAGAA AATGATGAAGAAATCAAACAGTTAGATGAAGAAATCAAGGAGCTAAATGAATCCAATTCCCAGATGGAA GCCGATATGATTAAACTCAGAACTCAGATTACCACGATGGAGAGCAACCTGAAGACCATCGAAGAGGAG AACAAAGTGATTGAGCAGCAGAACGAGTCTCTCCTCCACGAGCTGGCGAACCTGAGCCAGTCTCTGATC CACAGCCTGGCTAACATCCAGCTGCCGCACATGGATCCAATCAATGAACAAAATTTTGATGCTTACGTG ACTACTTTGACGGAAATGTATACAAATCAAGATCGTTATCAGAGTCCAGAAAATAAAGCCCTACTGGAA AATATAAAGCAGGCTGTGAGAGGAATTCAGGTCTGA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAG GATGACGACGATAAGGTTTAAACGGCCGGCCGCGGT |
Restriction Sites | SgfI-NotI |
ACCN | NM_015025 |
Insert Size | 3555 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_015025.3 |
RefSeq Size | 7192 bp |
RefSeq ORF | 3555 bp |
Locus ID | 23040 |
UniProt ID | Q9UL68 |
Cytogenetics | 2p25.3 |
Protein Families | Transcription Factors |
MW | 132.9 kDa |
Summary | This gene encodes a member of the zinc finger superfamily of transcription factors whose expression, thus far, has been found only in neuronal tissues. The encoded protein belongs to a novel class of cystein-cystein-histidine-cystein zinc finger proteins that function in the developing mammalian central nervous system. Forced expression of this gene in combination with the basic helix-loop-helix transcription factor NeuroD1 and the transcription factors POU class 3 homeobox 2 and achaete-scute family basic helix-loop-helix transcription factor 1 can convert fetal and postnatal human fibroblasts into induced neuronal cells, which are able to generate action potentials. Mutations in this gene have been associated with an autosomal dominant form of cognitive disability and with autism spectrum disorder. Alternative splicing results in multiple variants. [provided by RefSeq, Jul 2017] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
---|---|---|---|---|
RC221586 | MYT1L (Myc-DDK-tagged)-Human myelin transcription factor 1-like (MYT1L) | 10 ug |
$1,546.00
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RC221586L1 | Lenti ORF clone of Human myelin transcription factor 1-like (MYT1L), Myc-DDK-tagged | 10 ug |
$1,846.00
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RC221586L2 | Lenti ORF clone of Human myelin transcription factor 1-like (MYT1L), mGFP tagged | 10 ug |
$1,846.00
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RC221586L3 | Lenti ORF clone of Human myelin transcription factor 1-like (MYT1L), Myc-DDK-tagged | 10 ug |
$1,846.00
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RC221586L4 | Lenti ORF clone of Human myelin transcription factor 1-like (MYT1L), mGFP tagged | 10 ug |
$1,846.00
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RG221586 | MYT1L (tGFP-tagged) - Human myelin transcription factor 1-like (MYT1L) | 10 ug |
$1,746.00
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Citations
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.