AP4E1 (NM_007347) Human Untagged Clone

SKU SC303918

AP4E1 (untagged)-Human adaptor-related protein complex 4, epsilon 1 subunit (AP4E1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: AP4E1
• Synonyms: CPSQ4; SPG51; STUT1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007347 edited; GGCAGCGGCGGCCGGGCATGAAGCCGGGCGGCTACGGGATCGCGGGCGGCGGCGGCATCG; CGGGCGGCGGCGGCGATGAGCGACATAGTGGAGAAGACGCTGACGGCGCTGCCGGGACTC; TTTCTGCAGAACCAGCCCGGTGGTGGGCCCGCGGCCGCCAAGGCGTCCTTCTCCTCGAGG; CTGGGCAGCCTTGTCCGCGGCATCACAGCCCTCACCTCCAAGCACGAAGAAGAAAAATTA; ATCCAGCAGGAACTGAGTAGTCTGAAAGCGACTGTTTCTGCTCCTACTACAACACTGAAA; ATGATGAAGGAATGTATGGTGAGACTTATATATTGTGAAATGCTTGGATATGATGCTTCC; TTTGGCTATATTCATGCAATCAAGTTAGCCCAACAAGGAAACCTCTTAGAAAAAAGAGTA; GGTTATTTGGCTGTTTCCTTATTTCTACATGAAAGTCATGAATTATTGCTTCTCCTTGTG; AATACAGTTGTAAAGGATCTGCAGAGCACTAACCTAGTAGAAGTGTGTATGGCACTGACT; GTTGTTAGCCAGATTTTCCCCTGCGAAATGATTCCAGCTGTTCTTCCATTAATAGAAGAT; AAACTTCAACATTCTAAGGAGATTGTACGAAGAAAAGCTGTTCTGGCATTATACAAATTC; CATCTCATTGCTCCTAATCAAGTACAACATATTCATATTAAGTTTCGGAAAGCACTTTGT; GACAGAGATGTTGGGGTCATGGCTGCCTCCTTGCATATATATCTTAGAATGATTAAGGAG; AATTCATCTGGATATAAAGACTTGACTGGGAGTTTTGTAACCATTTTGAAGCAAGTAGTT; GGAGGAAAGCTCCCAGTAGAATTCAATTACCACAGTGTGCCAGCACCATGGTTACAAATT; CAGCTCTTGAGAATACTGGGACTTCTAGGAAAAGATGATCAAAGGACAAGTGAATTAATG; TATGATGTTCTTGATGAATCCTTACGAAGAGCTGAGTTAAATCACAATGTCACATATGCT; ATTTTGTTTGAATGTGTGCATACAGTCTATTCTATTTATCCTAAATCGGAATTACTTGAG; AAGGCTGCCAAGTGCATTGGAAAATTTGTTCTGTCACCTAAAATAAATCTAAAATATTTA; GGACTGAAGGCTCTTACCTATGTTATCCAACAGGATCCTACTCTGGCTCTTCAACACCAG; ATGACAATAATTGAATGTTTAGATCATCCTGATCCCATTATTAAAAGAGAGACTCTGGAA; CTTCTTTACAGAATTACTAATGCACAGAATATAACAGTTATTGTCCAGAAAATGCTTGAA; TATTTACATCAGAGCAAAGAAGAGTATGTCATCGTCAATTTGGTCGGCAAAATAGCAGAG; CTGGCTGAGAAATATGCTCCTGATAATGCATGGTTTATTCAGACAATGAATGCTGTGTTT; TCAGTAGGAGGAGATGTAATGCATCCTGATATTCCCAATAACTTTCTGAGACTACTAGCG; GAAGGTTTTGATGATGAAACAGAAGATCAGCAATTAAGACTCTATGCAGTTCAGTCTTAT; CTCACTTTACTGGATATGGAAAATGTGTTCTATCCACAGAGATTTCTTCAAGTTATGAGT; TGGGTATTAGGGGAATATTCCTACCTCTTAGATAAGGAAACGCCAGAGGAAGTTATAGCT; AAGCTCTACAAGTTACTTATGAATGACTCTGTGTCTTCAGAAACAAAAGCCTGGTTAATT; GCTGCTGTGACCAAATTGACATCTCAGGCGCACTCTTCTAATACAGTTGAGAGATTAATC; CATGAATTTACCATATCTTTGGATACTTGTATGAGACAACATGCATTTGAATTAAAACAT; TTGCATGAGAATGTGGAACTTATGAAGAGCTTGCTTCCAGTTGACAGGAGTTGTGAAGAC; TTGGTGGTAGATGCTTCTTTATCTTTTCTGGATGGTTTTGTGGCTGAAGGACTCAGTCAG; GGTGCAGCGCCTTACAAACCTCCCCATCAACGCCAGGAGGAAAAGCTTTCTCAGGAAAAA; GTTCTCAATTTTGAACCATATGGACTCTCCTTTTCTTCATCTGGCTTCACTGGACGACAG; TCTCCTGCTGGCATTTCTCTTGGTTCAGATGTATCTGGGAATAGTGCTGAGACAGGACTG; AAAGAGACAAATAGCTTGAAGCTGGAAGGTATAAAGAAATTGTGGGGGAAAGAAGGCTAT; CTTCCCAAGAAGGAAAGCAAAACTGGTGATGAAAGTGGAGCTCTGCCTGTTCCTCAAGAG; AGTATAATGGAGAATGTAGATCAAGCTATAACTAAAAAGGATCAATCTCAAGTTCTTACC; CAATCTAAAGAGGAGAAAGAAAAGCAGCTGCTGGCATCATCATTATTTGTTGGTCTAGGA; TCAGAAAGTACAATCAACCTGCTGGGAAAAGCAGATACTGTCTCTCACAAGTTCAGAAGG; AAATCAAAAGTCAAAGAAGCTAAAAGTGGCGAAACAACCAGTACTCATAATATGACCTGT; TCTTCCTTTAGTTCTTTGTCAAATGTGGCATATGAAGATGATTATTATTCGAATACTTTG; CACGATACAGGAGACAAGGAATTAAAGAAATTTTCTCTCACTTCAGAACTTTTGGATTCT; GAGTCACTCACAGAACTGCCCTTGGTTGAGAAATTCTCATATTGTAGTCTGTCTACACCT; TCATTGTTTGCTAATAACAACATGGAAATTTTTCACCCTCCTCAATCTACTGCAGCCTCA; GTTGCCAAGGAAAGCTCTTTAGCTTCATCTTTTTTGGAAGAAACTACTGAATACATACAC; TCAAATGCTATGGAAGTCTGTAATAATGAAACTATATCAGTGTCTTCTTATAAAATTTGG; AAAGATGATTGTTTATTGATGGTCTGGTCAGTCACTAATAAGAGTGGTTTGGAATTGAAA; AGTGCTGACTTAGAAATTTTTCCTGCAGAAAATTTCAAGGTGACTGAGCAACCTGGATGC; TGTTTGCCTGTAATGGAAGCAGAAAGCACCAAAAGCTTTCAATATAGTGTGCAGATAGAA; AAACCTTTTACAGAAGGAAATCTTACTGGTTTTATTAGTTATCATATGATGGATACTCAT; TCTGCTCAGCTGGAATTTTCTGTAAACTTATCACTATTAGATTTCATTAGACCATTAAAA; ATCTCAAGTGACGACTTTGGGAAACTCTGGTTATCCTTCGCAAATGATGTGAAACAAAAT; GTAAAAATGTCAGAATCTCAAGCTGCACTTCCTTCTGCACTAAAGACTCTGCAACAGAAA; CTAAGACTCCATATTATTGAGATTATAGGCAATGAAGGGCTATTGGCCTGTCAGCTGCTC; CCATCCATCCCCTGCTTACTGCATTGCCGAGTTCATGCAGATGTATTAGCCCTGTGGTTC; AGATCCTCCTGTTCTACTCTTCCTGACTATTTACTGTATCAGTGTCAAAAGGTGATGGAG; GGATCCTAGCAGAAGCCCTGCTAAATTTTACTCCATCAAGATCAATGGTTTACATAGATA; AACTTATTTACCAAAGTAAAAAGAACTCATGGTACTTCTAATGAAAATGGGGATTATTAC; AAGTGTGGTTTATATGTTTTCTTTATGATTCCTGGTCAAGAAAGATCCCCAAAACTGTAT; CCCTAACCTTTAACTCAGGATTGTACAGTATGTTTAGGTCCCTCAAAAAGTGACCTAAGC; TAATGTTATAAACTGCTAATGATTTATATATCACTTAGTGTGTAGAGGGACTGAAAATAT; TTATTTTGTTATAAATAATTTTATAGCACGTTTACTCTAGTGCTAGCTAATTTGTAATAA; AGCCAAGTCTCAGTTTTCTGCATTAAATGGAAGGGAGACATGAAATTGATAATCTCAAAC; TTAATTCATATTTGGCTTTGGAATGTAGTGTATGGTTTTTGGTAGGGAAGTCAATATTTT; CGATTATGTTTTGCTTAGATCAGTGTTGAACTAAGTTGGCATAGCACACACTAACAGTTG; TAGGAGATCCATGAGCATGCTGGA;
• Restriction Sites: Please inquire
• ACCN: NM_007347
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_007347.3.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007347.3, NP_031373.2
• RefSeq Size: 6682 bp
• RefSeq ORF: 3414 bp
• Locus ID: 23431
• UniProt ID: Q9UPM8
• Cytogenetics: 15q21.2
• Protein Pathways: Lysosome
• Summary: This gene encodes a member of the adaptor complexes large subunit protein family. These proteins are components of the heterotetrameric adaptor protein complexes, which play important roles in the secretory and endocytic pathways by mediating vesicle formation and sorting of integral membrane proteins. The encoded protein is a large subunit of adaptor protein complex-4, which is associated with both clathrin- and nonclathrin-coated vesicles. Disruption of this gene may be associated with cerebral palsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]; Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).