COL13A1 (NM_005203) Human Untagged Clone

SKU SC303601

COL13A1 (untagged)-Human collagen, type XIII, alpha 1 (COL13A1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: COL13A1
• Synonyms: alpha 1 type XIII collagen; collagen, type XIII, alpha 1; collagen alpha-1(XIII) chain; COLXIIIA1; FLJ42485; OTTHUMP00000060587
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303601 representing NM_005203.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCGCGCCC; GGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGG; CTGCTGACGCTGGCCCTCTGCTCGCTGGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAG; GCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATTTTGGGACGAGTC; AATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCA; GGATGTAACTGCCCACCAGCATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCC; ATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCATCATTGGTCCCCGCGGCCCC; CCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGC; CACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAA; GGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCCTAAGCAGCATGCCAGCAGCTCTGCGC; TCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTC; ATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGC; CCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCT; GGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATG; CCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAG; CCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGG; CAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGG; CTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCA; GGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAA; CCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTG; GCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAG; AAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGA; GACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAAC; GGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAG; AAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCT; CCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGA; GAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGAC; GGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAG; AGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGA; CCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAG; GGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGC; TGCTGGAACAAGTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_005203
• Insert Size: 2154 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005203.3
• RefSeq Size: 3136 bp
• RefSeq ORF: 2154 bp
• Locus ID: 1305
• Cytogenetics: 10q22.1
• MW: 70 kDa
• Summary: This gene encodes the alpha chain of one of the nonfibrillar collagens. The function of this gene product is not known, however, it has been detected at low levels in all connective tissue-producing cells so it may serve a general function in connective tissues. Unlike most of the collagens, which are secreted into the extracellular matrix, collagen XIII contains a transmembrane domain and the protein has been localized to the plasma membrane. The transcripts for this gene undergo complex and extensive splicing involving at least eight exons. Like other collagens, collagen XIII is a trimer; it is not known whether this trimer is composed of one or more than one alpha chain isomer. A number of alternatively spliced transcript variants have been described, but the full length nature of some of them has not been determined. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes isoform 1.