GCM2 (NM_004752) Human Untagged Clone

SKU
SC303528
GCM2 (untagged)-Human glial cells missing homolog 2 (Drosophila) (GCM2)
  $708.00
In Stock*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GCM2
Synonyms FIH2; GCMB; hGCMb; HRPT4
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_004752 edited
GCGCACCTGTCCTTTTTTCTTCTATTTTTCAGAACCCTGGGCGGAAAGCGCGAGCGAGTT
GGGCAGATGCCGGCGGCCGCGGTGCAGGAAGCGGTCGGCGTGTGCTCCTACGGGATGCAG
CTCAGCTGGGACATCAACGATCCGCAGATGCCTCAGGAGCTGGCCCTCTTTGACCAATTC
CGAGAGTGGCCTGACGGCTATGTGCGCTTCATCTACAGCAGCGATGAGAAGAAGGCACAG
CGTCACCTGAGCGGCTGGGCCATGCGCAACACCAACAACCACAATGGCCACATCCTCAAG
AAGTCGTGCCTGGGTGTGGTGGTGTGTACACAGGCCTGCACCCTGCCCGACGGTTCCCGC
CTGCAGCTGAGGCCGGCCATCTGCGACAAGGCACGGCTGAAACAGCAGAAGAAGGCATGC
CCTAACTGTCATTCTGCTTTGGAGTTGATTCCTTGTCGAGGGCACAGCGGATACCCCGTA
ACCAACTTTTGGCGGCTTGATGGCAACGCGATCTTTTTTCAGGCCAAGGGAGTTCATGAT
CATCCAAGACCAGAGAGCAAATCAGAGACAGAAGCTAGAAGAAGCGCCATCAAGAGACAA
ATGGCCTCTTTCTACCAACCCCAGAAAAAGAGAATTCGAGAATCCGAGGCAGAAGAAAAT
CAAGACAGCAGTGGTCATTTCAGCAACATACCTCCCTTGGAAAATCCAGAAGACTTTGAT
ATAGTTACTGAAACCAGCTTCCCTATTCCAGGGCAGCCTTGCCCTTCCTTCCCAAAGTCT
GATGTTTACAAAGCTACCTGTGACCTAGCCACCTTTCAAGGAGACAAAATGCCACCCTTC
CAGAAATACTCAAGCCCAAGAATCTATTTGCCTAGGCCACCTTGCAGCTATGAATTGGCA
AACCCTGGTTATACAAATTCAAGCCCATATCCCACCCTTTATAAGGATTCCACCAGTATC
CCTAATGACACAGACTGGGTTCATCTGAACACACTACAATGTAATGTCAATTCATACAGC
AGCTATGAGAGAAGCTTTGATTTCACCAACAAACAGCATGGCTGGAAACCAGCTCTTGGA
AAACCCAGCCTTGTGGAAAGGACTAACCATGGGCAGTTTCAGGCCATGGCCACTCGCCCT
TATTATAACCCAGAGCTTCCCTGCAGGTACCTCACGACTCCACCACCAGGTGCCCCTGCC
CTACAAACCGTGATCACCACCACCACTAAAGTGTCCTACCAGGCCTACCAGCCCCCTGCT
ATGAAATACAGTGACAGTGTGCGAGAGGTGAAGAGCCTTTCGAGCTGTAACTATGCTCCT
GAAGATACTGGGATGTCTGTCTATCCAGAACCCTGGGGTCCTCCGGTGACAGTCACCAGG
GCAGCCTCTCCTTCAGGGCCACCTCCTATGAAAATTGCAGGAGATTGCCGGGCCATCAGA
CCCACTGTGGCTATTCCCCACGAGCCAGTTTCCTCTAGGACAGATGAAGCAGAGACTTGG
GATGTGTGTCTGTCTGGGCTGGGCTCCGCAGTCAGTTACTCAGACAGAGTGGGTCCCTTC
TTTACCTACAACAATGAGGATTTTTGAAAGACAATCCAGGGGACA
Restriction Sites Please inquire |
ACCN NM_004752
Insert Size 1600 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004752.1, NP_004743.1
RefSeq Size 2373 bp
RefSeq ORF 1521 bp
Locus ID 9247
UniProt ID O75603
Cytogenetics 6p24.2
Summary This gene is a homolog of the Drosophila glial cells missing gene, which is thought to act as a binary switch between neuronal and glial cell determination. The protein encoded by this gene contains a conserved N-terminal GCM motif that has DNA-binding activity. The protein is a transcription factor that acts as a master regulator of parathyroid development. It has been suggested that this transcription factor might mediate the effect of calcium on parathyroid hormone expression and secretion in parathyroid cells. Mutations in this gene are associated with hypoparathyroidism. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_004752" in other vectors (4)
SKU Description Size Price
RC210179 GCM2 (Myc-DDK-tagged)-Human glial cells missing homolog 2 (Drosophila) (GCM2) 10 ug
$706.00
RC210179L3 Lenti-ORF clone of GCM2 (Myc-DDK-tagged)-Human glial cells missing homolog 2 (Drosophila) (GCM2) 10 ug
$1,006.00
RC210179L4 Lenti-ORF clone of GCM2 (mGFP-tagged)-Human glial cells missing homolog 2 (Drosophila) (GCM2) 10 ug
$1,006.00
RG210179 GCM2 (tGFP-tagged) - Human glial cells missing homolog 2 (Drosophila) (GCM2) 10 ug
$906.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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