ITGA8 (NM_003638) Human Untagged Clone

SKU SC303358

ITGA8 (untagged)-Human integrin, alpha 8 (ITGA8)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ITGA8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303358 representing NM_003638.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGTCGCCCGGGGCCAGCCGCGGTCCCCGGGGAAGCCAGGCGCCGCTGATCGCGCCCCTCTGCTGCGCC; GCGGCCGCGCTGGGGATGTTGCTGTGGTCCCCCGCCTGTCAGGCGTTCAACCTGGACGTGGAAAAGCTC; ACAGTGTACAGCGGCCCCAAGGGCAGCTACTTCGGCTACGCCGTGGACTTCCACATACCCGACGCCCGC; ACAGCGAGTGTCTTGGTGGGGGCGCCCAAAGCCAACACCAGCCAGCCCGATATCGTGGAAGGGGGAGCC; GTCTATTACTGTCCTTGGCCCGCGGAGGGGTCTGCGCAGTGCAGGCAGATACCGTTTGACACCACCAAC; AACAGAAAGATCAGAGTTAATGGAACCAAAGAACCTATCGAGTTCAAATCCAATCAGTGGTTTGGAGCA; ACAGTGAAAGCTCACAAAGGAAAAGTTGTGGCCTGTGCTCCTTTATATCACTGGAGAACTCTTAAACCG; ACACCAGAAAAGGACCCAGTTGGCACCTGCTATGTAGCAATTCAGAACTTCAGCGCCTATGCCGAGTTC; TCTCCTTGCCGGAACAGCAATGCTGATCCGGAAGGCCAGGGTTACTGCCAAGCAGGATTTAGTCTGGAT; TTTTATAAGAATGGAGACCTTATTGTGGGAGGACCTGGGAGTTTCTACTGGCAAGGACAAGTGATCACT; GCCAGTGTTGCAGATATCATTGCAAATTACTCATTCAAGGATATCCTCAGGAAACTGGCAGGAGAAAAG; CAGACGGAAGTGGCTCCAGCTTCCTATGATGACAGTTACCTTGGATACTCAGTTGCTGCTGGGGAGTTT; ACTGGGGATTCTCAGCAAGAATTGGTTGCTGGAATTCCAAGAGGAGCACAGAATTTTGGATATGTTTCC; ATCATTAACTCTACGGATATGACGTTTATTCAGAATTTCACGGGAGAACAGATGGCATCTTATTTTGGA; TATACCGTTGTCGTATCAGATGTTAACAGTGATGGACTGGATGATGTCCTGGTTGGGGCACCTCTCTTT; ATGGAACGTGAATTTGAGAGCAACCCCAGAGAAGTAGGGCAAATCTACCTGTATTTGCAAGTGAGCTCT; CTCCTCTTCAGAGACCCCCAGATCCTCACTGGCACCGAGACGTTTGGGAGATTCGGTAGTGCTATGGCA; CACTTAGGAGACCTGAACCAAGATGGATACAATGACATTGCCATCGGAGTGCCTTTTGCAGGCAAGGAT; CAAAGAGGCAAAGTGCTCATTTATAATGGGAACAAAGATGGCTTAAACACCAAGCCTTCCCAAGTTCTG; CAAGGAGTGTGGGCCTCACATGCTGTCCCTTCCGGATTTGGCTTTACTTTAAGAGGAGATTCAGACATA; GACAAGAATGATTACCCAGATTTGATTGTGGGTGCATTTGGAACAGGAAAAGTCGCTGTTTACAGAGCA; AGACCGGTTGTGACTGTAGATGCCCAGCTTCTGCTGCACCCAATGATTATCAATCTTGAAAATAAAACT; TGCCAGGTTCCAGACTCTATGACATCTGCTGCCTGCTTTTCTTTAAGAGTATGTGCATCTGTCACAGGC; CAGAGCATTGCAAACACAATAGTCTTGATGGCAGAGGTGCAATTAGATTCCCTGAAACAGAAAGGAGCT; ATTAAACGGACGCTCTTCCTTGATAACCATCAGGCTCATCGCGTCTTCCCTCTTGTGATAAAAAGGCAG; AAATCCCACCAGTGCCAGGATTTCATCGTTTACCTTCGAGATGAAACTGAATTCCGAGATAAATTATCT; CCAATCAACATTAGTTTGAATTACAGTTTGGACGAATCCACCTTTAAAGAAGGCCTGGAAGTGAAACCA; ATATTGAACTACTACAGAGAAAACATTGTTAGTGAACAGGCTCACATTCTGGTGGACTGTGGAGAAGAC; AATCTGTGTGTTCCTGACTTGAAGCTGTCGGCTAGACCAGATAAGCATCAGGTAATCATTGGAGATGAA; AATCACCTTATGCTCATAATAAATGCAAGAAATGAAGGGGAAGGAGCATATGAAGCTGAACTCTTTGTA; ATGATACCAGAAGAGGCAGATTATGTTGGAATCGAACGCAACAACAAGGGATTTCGACCACTGAGCTGT; GAGTACAAGATGGAAAATGTAACCAGGATGGTGGTGTGTGACCTTGGGAACCCTATGGTGTCTGGAACA; AATTATTCCCTGGGCCTCCGATTTGCAGTTCCACGTCTTGAGAAAACAAACATGAGCATTAACTTCGAT; CTCCAAATCAGAAGTTCCAACAAGGACAATCCAGACAGCAATTTTGTGAGCCTGCAAATCAACATCACT; GCTGTAGCGCAGGTGGAAATAAGAGGAGTGTCACACCCTCCGCAGATTGTTCTGCCCATTCATAACTGG; GAACCAGAAGAGGAGCCCCACAAAGAGGAGGAGGTTGGACCATTGGTGGAACATATTTATGAGCTGCAC; AATATTGGACCAAGTACCATCAGTGACACCATCCTGGAGGTGGGCTGGCCTTTCTCTGCCCGGGATGAA; TTTCTTCTCTATATTTTCCATATTCAAACTCTGGGACCTCTGCAGTGCCAACCAAATCCTAATATCAAT; CCACAGGATATAAAGCCTGCTGCCTCCCCAGAGGACACCCCTGAGCTCAGCGCCTTTTTGCGAAACTCT; ACTATTCCTCATCTTGTCAGGAAGAGGGATGTACATGTGGTCGAATTCCACAGACAGAGCCCTGCAAAA; ATACTGAATTGTACAAATATCGAGTGTTTACAAATCTCCTGTGCAGTGGGACGACTCGAAGGAGGAGAA; AGCGCAGTCCTGAAAGTCAGGTCACGATTATGGGCCCACACCTTCCTCCAGAGAAAAAATGATCCCTAT; GCTCTTGCATCCCTGGTGTCCTTTGAAGTTAAGAAGATGCCTTATACAGATCAGCCAGCAAAACTCCCA; GAAGGAAGCATAGCAATTAAGACATCAGTTATTTGGGCAACTCCGAATGTTTCCTTCTCAATCCCATTA; TGGGTAATAATACTAGCAATACTTCTTGGATTGTTGGTTCTCGCCATTTTAACCTTAGCTTTATGGAAG; TGTGGATTCTTTGACAGAGCCAGACCTCCTCAGGAGGACATGACCGACAGGGAACAGCTGACAAATGAC; AAGACCCCTGAGGCATGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_003638
• Insert Size: 3192 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003638.2
• RefSeq Size: 6982 bp
• RefSeq ORF: 3192 bp
• Locus ID: 8516
• UniProt ID: P53708
• Cytogenetics: 10p13
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• MW: 117.4 kDa
• Summary: Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.