PALM2AKAP2 (NM_001037293) Human Untagged Clone

SKU SC302877

PALM2 (untagged)-Human paralemmin 2 (PALM2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: PALM2AKAP2
• Synonyms: AKAP-2; AKAP-KL; AKAP2; AKAPKL; MISP2; PALM2; PALM2-AKAP2; PRKA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302877 representing NM_001037293.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGAGATGGCAGAGGCGGAATTGCACAAGGAAAGGCTGCAAGCCATAGCAGAAAAAAGAAAGAGGCAG; ACTGAAATAGAAGGCAAGCGACAACAGCTTGACGAGCAGATACTTCTGCTGCAGCATTCCAAGTCCAAA; GTGCTTCGGGAGAAATGGCTGCTGCAGGGCATACCCGCTGGAACTGCCGAAGAGGAGGAAGCCAGGAGG; CGGCAGTCTGAAGAGGATGAGTTCAGAGTCAAGCAACTTGAAGATAACATTCAGAGGCTGGAGCAAGAA; ATACAAACGCTAGAAAGTGAAGAGTCCCAGATATCTGCCAAAGAGCAAATCATCCTAGAGAAACTGAAG; GAAACAGAAAAATCCTTCAAGGACTTTCAGAAGGGTTTCTCCAGTACGGATGGAGCTGTGTACGCCATG; GAAATTAATGTGGAGAAAGACAAACAAACAGGAGAGACCAAGATTCTCTCTACATCTACCATTGGCCCA; GAGGGGGTCCATCAGAAAGGAGTCAAAGTCTATGATGATGGTACCAAAGTAGTGTATGAGGTGCGCTCA; GGAGGCACCGTAGTAGAAAATGGAGTGCACAAATTAAGCACAAAGGATGTAGAAGAGCTTATTCAGAAG; GCTGGACAATCAAGCTTAGGAGGAGGGCACGTGTCTGAAAGGACTGTGATTGCAGATGGGAGCCTCAGC; CATCCCAAGGAACACATGCTCTGCAAAGAAGCTAAGTTAGAAATGGTACATAAGTCTAGGAAAGACCAT; TCTTCCGGGAACCCAGGGCAGCAGGCCCAAGCCCCCAGCGCTGCAGGGCCGGAGGCAAACTTGGATCAG; CCCGTCACCATGATTTTTATGGGCTACCAAAATATCGAGGATGAAGAGGAGACGAAAAAGGTGCTAGGC; TATGATGAAACCATCAAGGCTGAATTGGTCCTCATTGATGAAGATGATGAGAAGTCATTGAGGGAGAAG; ACAGTGACGGACGTGTCCACTATTGACGGGAACGCGGCTGAGCTTGTGTCCGGGAGGCCGGTCTCAGAC; ACCACAGAGCCCTCATCCCCAGAAGGGAAGGAAGAGAGCCTAGCTACAGAGCCAGCCCCAGGTACCCAA; AAGAAAAAGCGCTGTCAATGCTGTGTTGTCATGTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001037293
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001037293.2
• RefSeq Size: 9286 bp
• RefSeq ORF: 1140 bp
• Locus ID: 445815
• UniProt ID: Q8IXS6
• Cytogenetics: 9q31.3
• MW: 42.2 kDa
• Summary: This gene belongs to the paralemmin downstream gene (PDG) family defined in PMID:22855693. Paralemmin downstream genes may have evolved contiguously with the paralemmin genes and are associated with other paralemmin paralogs in humans and several other taxa. The gene encodes three distinct protein isoforms, the PALM2 isoform, the AKAP2 isoform and the PALM2-AKAP2 isoform. The biological significance of the PALM2-AKAP2 isoforms is yet unknown. Earlier, PALM2 and AKAP2 were annotated as separate genes and PALM2-AKAP2 was annotated as a readthrough gene. [provided by RefSeq, May 2019]; Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting protein, isoform 2 (PALM2), has a distinct N-terminus and is shorter than isoform 1 (PALM2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.