VEGFA (NM_001025366) Human Untagged Clone
SKU
SC302394
VEGFA (untagged)-Human vascular endothelial growth factor A (VEGFA), transcript variant 1
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | VEGFA |
| Synonyms | MVCD1; VEGF; VPF |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Restriction Sites | Please inquire | |
| ACCN | NM_001025366 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | There are no nucleotide differences between the OriGene clone and the NCBI reference ORF. Please note the alternative use of CTG as the initiation codon. A protein BLAST will incorrectly interpret this codon as coding for a leucine residue. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001025366.1, NP_001020537.2 |
| RefSeq Size | 3665 bp |
| RefSeq ORF | 1239 bp |
| Locus ID | 7422 |
| UniProt ID | P15692 |
| Cytogenetics | 6p21.1 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Bladder cancer, Cytokine-cytokine receptor interaction, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma, VEGF signaling pathway |
| Summary | This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation from upstream non-AUG (CUG) codons resulting in additional isoforms. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic. Expression of some isoforms derived from the AUG start codon is regulated by a small upstream open reading frame, which is located within an internal ribosome entry site. The levels of VEGF are increased during infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), thus promoting inflammation by facilitating recruitment of inflammatory cells, and by increasing the level of angiopoietin II (Ang II), one of two products of the SARS-CoV-2 binding target, angiotensin-converting enzyme 2 (ACE2). In turn, Ang II facilitates the elevation of VEGF, thus forming a vicious cycle in the release of inflammatory cytokines. provided by RefSeq, Jun 2020 Transcript Variant: This variant (1) represents the longest transcript. This variant can initiate translation from four non-AUG (CUG) sites, and also from a downstream, in-frame AUG. The isoform (a) represented in this RefSeq is derived from the 5'-most CUG start codon. This is the longest isoform. CCDS Note: This CCDS representation starts at an upstream non-AUG (CUG) translation start codon that is supported by conservation data and experimental evidence, including that in PMIDs:11352659, 11563986 and 11731620. Most of the publicly available long mRNAs at this locus do not extend far enough 5' to include the CUG start codon, including S85192.1 which supports this variant. However, BC058855.1, several unspliced partial transcripts and the aforementioned publications all support transcription initiation further upstream. Hence, this CCDS representation includes the extended 5' CDS. Alternative translation initiation from a downstream AUG codon (Met-181), which results in a shorter isoform with a predicted signal peptide, has also been shown for this gene. The shorter isoform encoded by this variant is represented by CCDS 55010.1. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001025366" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC223789 | VEGFA (Myc-DDK-tagged)-Human vascular endothelial growth factor A (VEGFA), transcript variant 1 | 10 ug |
$686.00
|
|
| RC223789L1 | Lenti-ORF clone of VEGFA (Myc-DDK-tagged)-Human vascular endothelial growth factor A (VEGFA), transcript variant 1 | 10 ug |
$986.00
|
|
| RC223789L2 | Lenti-ORF clone of VEGFA (mGFP-tagged)-Human vascular endothelial growth factor A (VEGFA), transcript variant 1 | 10 ug |
$986.00
|
|
| RC223789L3 | Lenti-ORF clone of VEGFA (Myc-DDK-tagged)-Human vascular endothelial growth factor A (VEGFA), transcript variant 1 | 10 ug |
$986.00
|
|
| RC223789L4 | Lenti-ORF clone of VEGFA (mGFP-tagged)-Human vascular endothelial growth factor A (VEGFA), transcript variant 1 | 10 ug |
$986.00
|
|
| RG223789 | VEGFA (tGFP-tagged) - Human vascular endothelial growth factor A (VEGFA), transcript variant 1 | 10 ug |
$886.00
|
|
Citations
A bioactive nano-calcium phosphate paste for in-situ transfection of BMP-7 and VEGF-A in a rabbit critical-size bone defect: results of an in vivo study
Schlickewei, Klatte, Wildermuth et al
J Mater Sci Mater Med (2019) 30 (2), 15 DOI: 10.1007/s10856-019-6217-y
Schlickewei, Klatte, Wildermuth et al
J Mater Sci Mater Med (2019) 30 (2), 15 DOI: 10.1007/s10856-019-6217-y
AI Summary: In this study, OriGene's plasmids pCMV6-XL4 (BMP-7) and pCMV6-XL5 (VEGF-A) were used to encode and deliver BMP-7 and VEGF-A genes within a nano-calcium phosphate paste, enabling in-situ transfection that significantly accelerated bone healing in a rabbit bone defect model.
Snippet: For the expression of BMP-7 and VEGF-A, the plasmid pCMV6-XL4 (Homo sapiens bone morphogenetic protein 7, OriGene, Rockville, USA) and the plasmid pCMV6-XL5 (Homo sapiens vascular endothelial growth factor A, transcript variant 4, OriGene, Rockville, USA
High Concentrations of Uric Acid Inhibit Angiogenesis via Regulation of the Krüppel-Like Factor 2-Vascular Endothelial Growth Factor-A Axis by miR-92a
Yu, Hong, Wang et al
Circ. J. (2015)
Yu, Hong, Wang et al
Circ. J. (2015)
AI Summary: OriGene's VEGFA cDNA expression vector was used to overexpress VEGFA in endothelial cells, helping the authors investigate the role of VEGFA in uric acid-mediated inhibition of angiogenesis.
Snippet: A scram- bled siRNA was used as a control (5'- UCAAGAAGC- CAAGGAUAAU- 3'). For overexpression of VEGFA, HUVECs were transfected with vectors containing VEGFA cDNA (Origene, Beijing, China) using LipofectamineTM 2000, according to the manufacturer's ... Cite SaveSaving
p75(NTR)-dependent activation of NF-κB regulates microRNA-503 transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia
Caporali, Meloni, Nailor et al
Nat Commun (2015) 6, 8024 DOI: 10.1038/ncomms9024
Caporali, Meloni, Nailor et al
Nat Commun (2015) 6, 8024 DOI: 10.1038/ncomms9024
Cited by 135
AI Summary: OriGene products, including the p75(NTR) expression plasmid and p75(NTR) siRNA, were used in this study to modulate p75(NTR) levels in endothelial cells, enabling the authors to investigate its role in activating NF-κB and regulating miR-503 transcription during diabetic vascular complications.
Prokaryotic Vector Construction, Protein Expression and Identification of Human Vascular Endothelial Growth Factor A (VEGFA)
Yu, Yue-xin, Min-nan et al
Journal of Kunming Medical University (2015) 36, 31-34
Yu, Yue-xin, Min-nan et al
Journal of Kunming Medical University (2015) 36, 31-34
Snippet: 1.1 主要材料 hVEGFA 的cDNA 购至美国Origene 公司． PCR 产物胶回收、 质粒纯化试剂盒购自北京天根 生物公司．兔抗人VEGFA 多克隆抗体购自abmart 公司．Western ECL 显影试剂盒、2x taq PCR master mix、山羊抗鼠IgG-HRP 均购自北京康为世纪公 司．cDNA 反转录盒 ... Cite SaveSaving
Showing 1-4 of 4 papers.
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