GLE1 (NM_001003722) Human Untagged Clone

SKU SC300534

GLE1 (untagged)-Human GLE1 RNA export mediator homolog (yeast) (GLE1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: GLE1
• Synonyms: CAAHC; CAAHD; GLE1L; hGLE1; LCCS; LCCS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC300534 representing NM_001003722.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCCGTCTGAGGGTCGCTGCTGGGAGACCTTGAAGGCCCTACGCAGTTCCGACAAAGGTCGCCTTTGC; TACTACCGCGACTGGCTGCTGCGGCGCGAGGATGTTTTAGAAGAATGTATGTCTCTTCCCAAGCTATCT; TCTTATTCTGGATGGGTGGTAGAGCACGTCCTACCCCATATGCAGGAGAACCAACCTCTGTCTGAGACT; TCGCCATCCTCTACGTCAGCTTCAGCCCTAGATCAACCCTCATTTGTTCCCAAATCTCCTGACGCAAGC; TCTGCCTTTTCCCCAGCCTCCCCTGCAACACCAAATGGAACCAAGGGCAAAGATGAGTCCCAGCACACA; GAATCTATGGTACTTCAGTCCTCACGGGGGATCAAAGTGGAAGGCTGCGTCCGAATGTACGAACTGGTA; CACAGAATGAAAGGAACAGAGGGCCTGAGGCTATGGCAGGAGGAGCAGGAGAGGAAGGTGCAAGCCCTC; TCGGAGATGGCATCTGAACAACTGAAGCGGTTTGATGAATGGAAGGAACTGAAGCAGCATAAAGAATTC; CAGGACTTGCGGGAAGTAATGGAGAAGAGCTCCAGAGAAGCCTTGGGACACCAAGAGAAGCTAAAAGCT; GAGCACCGTCACAGAGCAAAGATTCTCAACCTGAAGCTGCGGGAAGCAGAGCAGCAGCGCGTGAAGCAA; GCAGAACAGGAGCGGCTTCGGAAGGAAGAAGGCCAGATCCGCCTGCGGGCCCTCTATGCTCTGCAGGAG; GAGATGCTGCAGCTCAGCCAGCAGCTGGATGCCTCTGAGCAGCACAAAGCCCTGCTTAAGGTCGACCTG; GCTGCCTTCCAGACCCGAGGCAACCAGCTGTGCAGCCTCATCTCAGGGATCATCCGGGCCTCTTCAGAG; AGCAGCTATCCCACAGCAGAGAGTCAAGCTGAGGCTGAGCGAGCTCTGCGGGAAATGCGGGACCTCCTG; ATGAACTTGGGGCAGGAGATCACCAGAGCCTGCGAAGACAAGAGGAGGCAGGATGAAGAAGAGGCCCAG; GTAAAGCTGCAAGAGGCACAGATGCAGCAGGGACCAGAGGCCCACAAAGAGCCCCCAGCTCCCAGCCAG; GGCCCAGGAGGGAAACAGAATGAAGACCTCCAGGTGAAGGTACAAGACATTACAATGCAGTGGTACCAG; CAGCTGCAGGATGCTTCCATGCAGTGTGTGTTGACCTTTGAGGGCCTGACCAACAGCAAGGACAGTCAG; GCCAAAAAGATAAAGATGGACCTCCAGAAGGCTGCTACCATCCCAGTGAGCCAAATCTCTACCATTGCA; GGCTCAAAACTGAAGGAGATCTTTGACAAGATCCACAGCCTGCTCTCTGGAAAACCTGTTCAATCTGGT; GGGCGCTCTGTGTCTGTCACACTTAACCCACAGGGGCTGGACTTTGTTCAATACAAACTGGCAGAGAAA; TTTGTGAAACAAGGCGAGGAGGAAGTGGCCTCTCACCATGAAGCAGCATTCCCCATTGCAGTTGTGGCA; TCCGGGATCTGGGAGCTCCACCCCAGAGTGGGGGACCTCATTCTTGCTCATCTACATAAGAAGTGTCCT; TACTCTGTTCCTTTCTATCCCACTTTCAAGGAGGGAATGGCTTTGGAAGACTATCAGAGGATGCTTGGT; TACCAAGTAAAGGATTCCAAAGTGGAGCAGCAAGACAACTTTCTAAAACGCATGTCAGGGATGATCCGT; CTCTACGCTGCTATCATCCAGCTCCGGTGGCCATATGGAAACCGACAGGAGATTCACCCTCATGGCTTA; AATCATGGATGGCGCTGGTTGGCACAGATCTTAAACATGGAGCCCTTGTCAGATGTGACAGCCACCCTC; CTCTTTGACTTCCTGGAGGTGTGTGGGAATGCCCTCATGAAGCAATACCAGGTTCAGTTCTGGAAGATG; CTAATTCTCATCAAAGAGGACTACTTTCCCAGAATTGAAGCTATCACAAGCTCAGGACAGATGGGCTCC; TTCATACGCCTCAAGCAGTTCTTGGAGAAATGTTTGCAACACAAGGACATTCCTGTCCCCAAGGGCTTT; CTGACTTCCTCCTTCTGGCGCTCCTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001003722
• Insert Size: 2097 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001003722.1
• RefSeq Size: 3350 bp
• RefSeq ORF: 2097 bp
• Locus ID: 2733
• UniProt ID: Q53GS7
• Cytogenetics: 9q34.11
• MW: 79.8 kDa
• Summary: This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).