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ATP4A (NM_000704) Human Untagged Clone

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SKU
SC300122
ATP4A (untagged)-Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A)
  $904.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ATP4A
Synonyms ATP6A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC300122 representing NM_000704.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGGAAGGCCGAGAACTATGAGCTCTACTCGGTGGAGCTGGGTCCTGGCCCTGGCGGGGACATGGCT
GCCAAGATGAGCAAGAAGAAGAAGGCGGGTGGCGGGGGTGGCAAGAGGAAGGAGAAGCTGGAGAACATG
AAGAAGGAGATGGAGATTAACGACCACCAGCTGTCAGTGGCGGAGCTGGAACAGAAATACCAGACCAGT
GCCACCAAGGGCCTCTCTGCGAGCCTGGCTGCTGAGCTGCTGCTGCGGGATGGGCCCAACGCACTGCGG
CCACCACGGGGCACCCCAGAGTACGTCAAGTTTGCGAGGCAGCTGGCCGGGGGCCTGCAGTGCCTCATG
TGGGTTGCCGCCGCCATCTGCCTCATCGCCTTTGCCATCCAGGCTAGTGAGGGGGACCTCACCACCGAC
GACAATCTGTACCTGGCAATCGCTCTCATTGCTGTGGTTGTCGTCACCGGCTGCTTTGGCTACTACCAG
GAATTCAAGAGCACCAACATCATCGCCAGCTTTAAGAACCTTGTGCCACAGCAAGCCACTGTCATCCGC
GATGGAGACAAATTCCAGATCAACGCTGACCAACTGGTGGTGGGCGACCTGGTGGAGATGAAAGGTGGG
GACAGAGTGCCCGCCGACATCCGCATCCTGGCGGCCCAGGGCTGCAAGGTGGACAACTCCTCGCTGACA
GGGGAGTCTGAGCCACAGACCCGCTCACCCGAGTGCACGCACGAGAGCCCTCTGGAGACCCGCAACATC
GCCTTCTTCTCCACCATGTGCCTTGAGGGCACCGTGCAGGGCCTGGTGGTGAACACGGGCGACCGCACC
ATCATTGGGCGCATCGCATCGCTGGCGTCGGGGGTGGAAAACGAGAAGACACCCATCGCTATCGAGATC
GAGCATTTTGTGGACATCATCGCGGGCCTGGCCATTCTCTTCGGTGCCACATTTTTTATTGTGGCCATG
TGCATTGGCTACACCTTCCTGCGGGCCATGGTCTTCTTCATGGCCATCGTGGTGGCCTATGTGCCTGAG
GGGCTGCTGGCCACTGTCACAGTCTGCCTGTCCCTGACAGCCAAGCGCCTGGCCAGTAAGAACTGCGTG
GTCAAGAACCTGGAGGCGGTGGAGACATTGGGCTCCACTTCGGTGATCTGCTCGGACAAGACAGGGACT
CTCACTCAGAACCGCATGACTGTGTCCCATCTGTGGTTTGACAACCACATCCACACAGCTGACACCACG
GAAGACCAGTCAGGGCAGACGTTTGACCAGTCCTCGGAGACGTGGCGGGCGCTGTGCCGGGTGCTCACC
CTGTGCAACCGCGCCGCCTTCAAGTCCGGCCAGGATGCAGTGCCTGTGCCCAAGCGCATCGTGATTGGA
GACGCATCGGAGACGGCGCTGCTCAAGTTCTCGGAGCTGACGCTGGGCAACGCCATGGGCTACCGGGAC
CGCTTCCCAAAAGTCTGCGAGATACCCTTCAACTCCACCAACAAGTTCCAGCTGTCCATCCATACGCTG
GAGGACCCGCGGGACCCGCGACACTTGCTGGTGATGAAGGGCGCCCCCGAGCGCGTGCTGGAGCGCTGC
AGCTCCATCCTTATCAAGGGCCAGGAGCTGCCGCTGGACGAGCAGTGGCGCGAGGCCTTCCAGACCGCC
TACCTCAGCCTGGGAGGCCTGGGCGAACGCGTGCTCGGCTTCTGCCAGCTCTACCTGAATGAGAAGGAC
TACCCGCCTGGCTATGCCTTCGACGTAGAGGCCATGAACTTTCCATCTAGCGGCCTCTGCTTTGCGGGA
CTTGTATCCATGATTGACCCACCCCGGGCCACCGTCCCTGATGCTGTGCTCAAGTGTCGCACCGCAGGC
ATCCGGGTGATCATGGTAACGGGTGACCACCCCATCACCGCCAAGGCCATTGCAGCCAGTGTGGGCATC
ATCTCGGAAGGCAGCGAGACAGTGGAGGACATCGCTGCCCGCCTCCGTGTGCCCGTAGACCAGGTTAAT
CGCAAGGATGCCCGTGCCTGTGTGATCAATGGCATGCAGCTGAAGGACATGGACCCATCGGAACTGGTC
GAGGCCCTGCGCACCCACCCCGAGATGGTGTTTGCGCGCACCAGCCCCCAGCAGAAGCTGGTGATCGTG
GAGAGCTGCCAGCGGCTGGGTGCGATTGTGGCCGTCACGGGGGATGGTGTGAATGACTCCCCAGCTCTG
AAGAAGGCAGACATCGGAGTAGCCATGGGCATCGCTGGCTCAGATGCTGCCAAAAATGCAGCTGACATG
ATCCTGCTGGATGACAACTTTGCCTCCATTGTGACAGGCGTGGAGCAGGGTCGACTGATCTTCGACAAC
CTGAAGAAGTCTATTGCCTACACATTGACCAAGAACATCCCAGAGCTGACACCCTACCTCATCTACATC
ACCGTCAGCGTGCCCCTGCCCCTCGGGTGCATCACCATCCTCTTCATCGAACTCTGCACTGACATTTTC
CCATCTGTGTCCCTGGCATATGAAAAGGCCGAGAGTGACATCATGCACCTGCGTCCACGCAACCCAAAG
CGTGACAGATTGGTCAACGAGCCCCTGGCTGCCTACTCCTACTTCCAGATTGGTGCCATTCAGTCCTTT
GCTGGCTTCACTGACTACTTCACGGCAATGGCCCAGGAGGGCTGGTTCCCACTGCTGTGCGTGGGGCTG
CGGGCGCAGTGGGAGGACCACCACCTACAAGATCTGCAGGACAGCTACGGCCAGGAGTGGACATTCGGG
CAGCGCCTGTACCAGCAGTACACCTGCTACACCGTGTTCTTCATCAGCATTGAGGTGTGCCAGATCGCC
GATGTCCTCATCCGCAAGACGCGCCGTCTCTCTGCCTTCCAGCAAGGCTTCTTCAGGAATAAGATCCTG
GTGATCGCCATCGTGTTCCAGGTCTGCATCGGCTGCTTCCTGTGCTACTGCCCCGGCATGCCCAACATC
TTCAACTTCATGCCCATTCGGTTCCAGTGGTGGCTGGTCCCCCTGCCCTACGGCATCCTCATCTTCGTC
TATGATGAGATCCGGAAGCTTGGAGTTCGCTGTTGCCCAGGGAGCTGGTGGGACCAGGAACTCTACTA
TAG
AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII |
ACCN NM_000704
Insert Size 3117 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000704.2
RefSeq Size 3582 bp
RefSeq ORF 3117 bp
Locus ID 495
UniProt ID P20648
Cytogenetics 19q13.12
Protein Families Druggable Genome, Transmembrane
Protein Pathways Oxidative phosphorylation
MW 114.4 kDa
Summary The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. provided by RefSeq, Jul 2008
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"NM_000704" in other vectors (6)
SKU Description Size Price
RC211772 ATP4A (Myc-DDK-tagged)-Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A) 10 ug
$904.00
RC211772L1 Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), Myc-DDK-tagged 10 ug
$1,204.00
RC211772L2 Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), mGFP tagged 10 ug
$1,204.00
RC211772L3 Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), Myc-DDK-tagged 10 ug
$1,204.00
RC211772L4 Lenti ORF clone of Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A), mGFP tagged 10 ug
$1,204.00
RG211772 ATP4A (tGFP-tagged) - Human ATPase, H+/K+ exchanging, alpha polypeptide (ATP4A) 10 ug
$789.00 $1,104.00

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