DPM1 (NM_003859) Human Untagged Clone

SKU SC128254

DPM1 (untagged)-Human dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit (DPM1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DPM1
• Synonyms: CDGIE; MPDS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003859 edited; ATGGCCTCCTTGGAAGTCAGTCGTAGTCCTCGCAGGTCTCGGCGGGAGCTGGAAGTGCGC; AGTCCACGACAGAACAAATATTCGGTGCTTTTACCTACCTACAACGAGCGCGAGAACCTG; CCGCTCATCGTGTGGCTGCTGGTGAAAAGCTTCTCCGAGAGTGGAATCAACTATGAAATT; ATAATCATAGATGATGGAAGCCCAGATGGAACAAGGGATGTTGCTGAACAGTTGGAGAAG; ATCTATGGGTCAGACAGAATTCTTCTAAGACCACGAGAGAAAAAGTTGGGACTAGGAACT; GCATATATTCATGGAATGAAACATGCCACAGGAAACTACATCATTATTATGGATGCTGAT; CTCTCACACCATCCAAAATTTATTCCTGAATTTATTAGGAAGCAAAAGGAGGGTAATTTT; GATATTGTCTCTGGAACTCGCTACAAAGGAAATGGAGGTGTATATGGCTGGGATTTGAAA; AGAAAAATAATCAGCCGTGGGGCCAATTTTTTAACTCAGATCTTGCTGAGACCAGGAGCA; TCTGATTTAACAGGAAGTTTCAGATTATACCGAAAAGAAGTTCTAGAGAAATTAATAGAA; AAATGTGTTTCTAAAGGCTACGTCTTCCAGATGGAGATGATTGTTCGGGCAAGACAGTTG; AATTATACTATTGGCGAGGTTCCAATATCATTTGTGGATCGTGTTTATGGTGAATCCAAG; TTGGGAGGAAATGAAATAGTATCTTTCTTGAAAGGATTATTGACTCTTTTTGCTACTACA; TAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003859 unedited; NGGTTAGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGATGGCCTCC; TTGGAAGTCAGTCGTAGTCCTCGCAGGTCTCGGCGGGAGCTGGAAGTGCGCAGTCCACGA; CAGAACAAATATTCGGTGCTTTTACCTACCTACAACGAGCGCGAGAACCTGCCGCTCATC; GTGTGGCTGCTGGTGAAAAGCTTCTCCGAGAGTGGAATCAACTATGAAATTATAATCATA; GATGATGGAAGCCCAGATGGAACAAGGGATGTTGCTGAACAGTTGGAGAAGATCTATGGG; TCAGACAGAATTCTTCTAAGACCACGAGAGAAAAAGTTGGGACTAGGAACTGCATATATT; CATGGAATGAAACATGCCACAGGAAACTACATCATTATTATGGATGCTGATCTCTCACAC; CATCCAAAATTTATTCCTGAATTTATTAGGAAGCAAAAGGAGGGTAATTTTGATATTGTC; TCTGGAACTCGCTACAAAGGAAATGGAGGTGTATATGGCTGGGATTTGAAAAGAAAAATA; ATCAGCCGTGGGGCCAATTTTTTAACTCAGATCTTGCTGAGACCAGGAGCATCTGATTTA; ACAGGAAGTTTCAGATTATACCGAAAAGAAGTTCTAGAGAAATTAATAGAAAAATGTGTT; TCTAAAGGCTACGTCTTCCAGATGGAGATGATTGTTCGGGCAAGACAGTTGAATTATACT; ATTGGCGAGGTTCCAATATCATTTGTGGATCGTGTTTATGGTGAATCCAAGTTGGGAGGA; AATGAAATAGTATCTTTCTTNGAAGGATTATTGACTCTTTTTGCTACTACATAAAAGAAA; GATACTCATTTATAGTTACGTTCATTTCAGGTTAAACACTGAAGAAGCCTGGNTACTGAT; TTGTAN;
• Restriction Sites: Please inquire
• ACCN: NM_003859
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003859.1, NP_003850.1
• RefSeq Size: 1047 bp
• RefSeq ORF: 783 bp
• Locus ID: 8813
• UniProt ID: O60762
• Cytogenetics: 20q13.13
• Domains: Glycos_transf_2
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Summary: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]; Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.