alpha Synuclein (SNCA) (NM_007308) Human Untagged Clone

SKU SC128245

SNCA (untagged)-Human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 4

Product Data

• Type: Human Untagged Clone
• Target Symbol: alpha Synuclein
• Synonyms: NACP; PARK1; PARK4; PD1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007308 edited; CTTCTGCCTTTCCACCCTCGTGAGCGGAGAACTGGGAGTGGCCATTCGACGACAGTGTGG; TGTAAAGGAATTCATTAGCCATGGATGTATTCATGAAAGGACTTTCAAAGGCCAAGGAGG; GAGTTGTGGCTGCTGCTGAGAAAACCAAACAGGGTGTGGCAGAAGCAGCAGGAAAGACAA; AAGAGGGTGTTCTCTATGTAGGCTCCAAAACCAAGGAGGGAGTGGTGCATGGTGTGGCAA; CAGTGGCTGAGAAGACCAAAGAGCAAGTGACAAATGTTGGAGGAGCAGTGGTGACGGGTG; TGACAGCAGTAGCCCAGAAGACAGTGGAGGGAGCAGGGAGCATTGCAGCAGCCACTGGCT; TTGTCAAAAAGGACCAGTTGGGCAAGGAAGGGTATCAAGACTACGAACCTGAAGCCTAAG; AAATATCTTTGCTCCCAGTTTCTTGAGATCTGCTGACAGATGTTCCATCCTGTACAAGTG; CTCAGTTCCAATGTGCCCAGTCATGACATTTCTCAAAGTTTTTACAGTGTATCTCGAAGT; CTTCCATCAGCAGTGATTGAAGTATCTGTACCTGCCCCCACTCAGCATTTCGGTGCTTCC; CTTTCACTGAAGTGAATACATGGTAGCAGGGTCTTTGTGTGCTGTGGATTTTGTGGCTTC; AATCTACGATGTTAAAACAAATTAAAAACACCTAAGTGACTACCACTTATTTCTAAATCC; TCACTATTTTTTTGTTGCTGTTGTTCAGAAGTTGTTAGTGATTTGCTATCATATATTATA; AGATTTTTAGGTGTCTTTTAATGATACTGTCTAAGAATAATGACGTATTGTGAAATTTGT; TAATATATATAATACTTAAAAATATGTGAGCATGAAACTATGCACCTATAAATACTAAAT; ATGAAATTTTACCATTTTGCGATGTGTTTTATTCACTTGTGTTTGTATATAAATGGTGAG; AATTAAAATAAAACGTTATCTCATTGCAAAAATAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007308 unedited; GTTCAGTTCACAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCCTC; GTGCCGAATTCGGCACGAGGCTTCTGCCTTTCCACCCTCGTGAGCGGAGAACTGGGAGTG; GCCATTCGACGACAGTGTGGTGTAAAGGAATTCATTAGCCATGGATGTATTCATGAAAGG; ACTTTCAAAGGCCAAGGAGGGAGTTGTGGCTGCTGCTGAGAAAACCAAACAGGGTGTGGC; AGAAGCAGCAGGAAAGACAAAAGAGGGTGTTCTCTATGTAGGCTCCAAAACCAAGGAGGG; AGTGGTGCATGGTGTGGCAACAGTGGCTGAGAAGACCAAAGAGCAAGTGACAAATGTTGG; AGGAGCAGTGGTGACGGGTGTGACAGCAGTAGCCCAGAAGACAGTGGAGGGAGCAGGGAG; CATTGCAGCAGCCACTGGCTTTGTCAAAAAGGACCAGTTGGGCAAGGAAGGGTATCAAGA; CTACGAACCTGAAGCCTAAGAAATATCTTTGCTCCCAGTTTCTTGAGATCTGCTGACAGA; TGTTCCATCCTGTACAAGTGCTCAGTTCCAATGTGCCCAGTCATGACATTTCTCANAGTT; TTTACAGTGTATCTCGAAGTCNTCCATCAGCAGTGATTGAAGTATCTGTACCTGCCCCAC; TCAGCATTNTCGTGCTTCCCTTTCACTGAAGTGAATACATGGTAGCAGGNTCTTTGTGTG; CTGTGGATTTTGTGGCTTTCATCTACGATGGTAAAACCAATTANAAACACCCTAGTGACT; ACCACTTATTTCTAAATCCTCACTATTTTTTTGTTGCTGGTTGTCAAAAGTGGTAGTGAT; TTGCTATCATATATAATAAGATTTTTAGTGTCTTTTAATGAAACTGGCTAGAATTATGAC; CTATTGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007308 unedited; NCCCCTCGGAATGGCAACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGGATGC; CACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTT; TTTTTTTTTTTTTATTTTTGCAATGAGATAACGTTTTATTTTAATTCTCACCATTTATAT; ACAAACACAAGTGAATAAAACACATCGCAAAATGGTAAAATTTCATATTTAGTATTTATA; GGTGCATAGTTTCATGCTCACATATTTTTAAGTATTATATATATTAACAAATTTCACAAT; ACGTCATTATTCTTAGACAGTATCATTAAAAGACACCTAAAAATCTTATAATATATGATA; GCAAATCACTAACAACTTCTGAACAACAGCAACAAAAAAATAGTGAGGATTTAGAAATAA; GTGGTAGTCACTTAGGTGTTTTTAATTTGTTTTAACATCGTAGATTGAAGCCACAAAATC; CACAGCACACAAAGACCCTGCTACCATGTATTCACTTCAGTGAAAGGGAAGCACCGAAAT; GCTGAGTGGGGGCAGGTACAGATACTTCAATCACTGCTGATGGAAGACTTCGAGATACAC; TGTAAAAACTTTGAGAAATGTCATGACTGGGCACATTGGAACTGAGCACTTGTACAGGAT; GGAACATCTGTCAGCAGATCTCAAGAAACTGGGAGCAAAGATATTTCTTAGGCTTCAGGT; TCGTAGTCTTGATACCCTTCCTTGCCCAACTGGTCCTTTTTGACAAACCAGTGGCTGCTG; CAATGCTCCCTGCTCCCTCCACTGTCTTCTGGGCTACTGCTGTCACACCCGTCACCACTG; GCTCTCCAACATTTGTCACTTGCTCTTTGGGTCTCTCAGCCCTGTTGCCACACATGCACA; CTCCTNCTTGG;
• Restriction Sites: Please inquire
• ACCN: NM_007308
• Insert Size: 1000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007308.1, NP_009292.1
• RefSeq Size: 1096 bp
• RefSeq ORF: 339 bp
• Locus ID: 6622
• UniProt ID: P37840
• Cytogenetics: 4q22.1
• Domains: Synuclein
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Parkinson's disease
• Summary: Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]; Transcript Variant: This variant (4, also known as NACP112) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (NACP112) has the same N- and C-termini but is shorter compared to isoform NACP140. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.