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ATG5 (NM_004849) Human Untagged Clone

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SKU
SC128244
ATG5 (untagged)-Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5)
  $450.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ATG5
Synonyms APG5; APG5-LIKE; APG5L; ASP; hAPG5; SCAR25
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC128244 sequence for NM_004849 edited (data generated by NextGen Sequencing)
ATGACAGATGACAAAGATGTGCTTCGAGATGTGTGGTTTGGACGAATTCCAACTTGTTTC
ACGCTATATCAGGATGAGATAACTGAAAGGGAAGCAGAACCATACTATTTGCTTTTGCCA
AGAGTAAGTTATTTGACGTTGGTAACTGACAAAGTGAAAAAGCACTTTCAGAAGGTTATG
AGACAAGAAGACATTAGTGAGATATGGTTTGAATATGAAGGCACACCACTGAAATGGCAT
TATCCAATTGGTTTGCTATTTGATCTTCTTGCATCAAGTTCAGCTCTTCCTTGGAACATC
ACAGTACATTTTAAGAGTTTTCCAGAAAAAGACCTTCTGCACTGTCCATCTAAGGATGCA
ATTGAAGCTCATTTTATGTCATGTATGAAAGAAGCTGATGCTTTAAAACATAAAAGTCAA
GTAATCAATGAAATGCAGAAAAAAGATCACAAGCAACTCTGGATGGGATTGCAAAATGAC
AGATTTGACCAGTTTTGGGCCATCAATCGGAAACTCATGGAATATCCTGCAGAAGAAAAT
GGATTTCGTTATATCCCCTTTAGAATATATCAGACAACGACTGAAAGACCTTTCATTCAG
AAGCTGTTTCGTCCTGTGGCTGCAGATGGACAGTTGCACACACTAGGAGATCTCCTCAAA
GAAGTTTGTCCTTCTGCTATTGATCCTGAAGATGGGGAAAAAAAGAATCAAGTGATGATT
CATGGAATTGAGCCAATGTTGGAAACACCTCTGCAGTGGCTGAGTGAACATCTGAGCTAC
CCGGATAATTTTCTTCATATTAGTATCATCCCACAGCCAACAGATTGA

Clone variation with respect to NM_004849.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004849 unedited
GGGGGGGGNGGGGGGGAANNNNNNTTTTTCNNNNNNNNNGGTCAGNATTTGTNATACGAC
TCACTATAGGCGGCCGCGNAATTCGCACGAGGCTTCCTGGNATCCTGCTACCGCGTCCCC
GCAGAACAGTGTGTCAGGCGGGCAGCTTGCCCCGCCGCCCCACCGGAGCGCGGAATCTGG
GCGTCCCCACCAGTGCGGGGAGCCGGAAGGAGGAGCCATAGCTTGGAGTAGGTTTGGCTT
TGGTTGAAATAAGAATTTAGCCTGTATGTACTGCTTTAACTCCTGGAAGAATGACAGATG
ACAAAGATGTGCTTCGAGATGTGTGGTTTGGACGAATTCCAACTTGTTTCACGCTATATC
AGGATGAGATAACTGAAAGGGAAGCAGAACCATACTATTTGCTTTTGCCAAGAGTAAGTT
ATTTGACGTTGGTAACTGACAAAGTGAAAAAGCACTTTCAGAAGGTTATGAGACAAGAAG
ACATTAGTGAGATATGGTTTGAATATGAAGGCACACCACTGAAATGGCATTATCCAATTG
GTTTGCTATTTGATCTTCTTGCATCAAGTTCAGCTCTTCCTTGGAACATCACAGTACATT
TTAAGAGTTTTCCAGAAAAAGACCTTCTGCACTGTCCATCTAAGGATGCAATTGAAGCTC
ATTTTATGTCATGTATGAAAGAAGCTGATGCTTTAAAACATAAAAGTCAAGTAATCAATG
AAATGCAGAANAAAGATCACAAGCAACTCTGGATGGGATTGCAAAATGACAGATTTGACC
AGTTTTGGGCCATCAATCGNAAACTCATGGNATATCCTGCAGAAGAAAATGGATTTCGTT
ATATCCCCTTTAGATATATCANACNACGACTGANAGACCTTTCATTCAGAAGCTGTTTCG
TCCTGTGGCTGCANATGGCAGTTGCCACACTAN
3' Read Nucleotide Sequence
>Forward primer walk for NM_004849 unedited
NAAGGTTCATTTNAAAATTTTCCANAAAAAACCTCTGCCTGTCCACTAAGGATGCAATTG
AAGCTCATTTTATGTCATGTATGAAAGAAGCTGATGCTTTAAAACATAAAAGTCAAGTAA
TCAATGAAATGCAGAAAAAAGATCACAAGCAACTCTGGATGGGATTGCAAAATGACAGAT
TTGACCAGTTTTGGGCCATCAATCGGAAACTCATGGAATATCCTGCAGAAGAAAATGGAT
TTCGTTATATCCCCTTTAGAATATATCAGACAACGACTGAAAGACCTTTCATTCAGAAGC
TGTTTCGTCCTGTGGCTGCAGATGGACAGTTGCACACACTAGGAGATCTCCTCAAAGAAG
TTTGTCCTTCTGCTATTGATCCTGAAGATGGGGAAAAAAAGAATCAAGTGATGATTCATG
GAATTGAGCCAATGTTGGAAACACCTCTGCAGTGGCTGAGTGAACATCTGAGCTACCCGG
ATAATTTTCTTCATATTAGTATCATCCCACAGCCAACAGATTGAAGGATCAACTATTTGC
CTGAACAGAATCATCCTTAAATGGGATTTATCAGAGCATGTCACCCTTTTGCTTCAATCA
GGTTTGGTGGAGGCAACCTGACCAGAAACACTTCGCTGCTGCAAGCCAGACAGGAAAAAG
ATTCCATGTCAGATAAGGCAACTGGGCTGGTCTTACTTTGCATCACCTCTGCTTTCCTCC
ACTGCCATCATTAAACCTCAGCTGTGACATGAAAGACTTACCGGACCACTNGAAGGTCTT
CTGNTAAATATAATGAAGCTGANACCTTTGGCCTAAGAAGAAATGGNAGTATGTGCCACT
CGATTTGTATTTCTGATTAAAAAATAACAGGGGTATTTCCTAANGTGACCATGGNTTGAA
CTTAGCTCATG
Restriction Sites NotI-NotI |
ACCN NM_004849
Insert Size 3400 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004849.2, NP_004840.1
RefSeq Size 3244 bp
RefSeq ORF 828 bp
Locus ID 9474
UniProt ID Q9H1Y0
Cytogenetics 6q21
Domains APG5
Protein Families Druggable Genome
Protein Pathways Regulation of autophagy, RIG-I-like receptor signaling pathway
Summary The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene. provided by RefSeq, Sep 2015
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Both variants 1 and 2 encode isoform a.
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"NM_004849" in other vectors (6)
SKU Description Size Price
RC210563 ATG5 (Myc-DDK-tagged)-Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5) 10 ug
$450.00
RC210563L1 Lenti ORF clone of Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5), Myc-DDK-tagged 10 ug
$750.00
RC210563L2 Lenti ORF clone of Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5), mGFP tagged 10 ug
$750.00
RC210563L3 Lenti ORF clone of Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5), Myc-DDK-tagged 10 ug
$750.00
RC210563L4 Lenti ORF clone of Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5), mGFP tagged 10 ug
$750.00
RG210563 ATG5 (tGFP-tagged) - Human ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5) 10 ug
$650.00

file-alt Citations

Novel protein complexes containing autophagy and UPS components regulate proteasome-dependent PARK2 recruitment onto mitochondria and PARK2-PARK6 activity during mitophagy
Kocaturk, Peker, Eberhart et al
Cell Death Dis (2022) 13 (11), 947 DOI: 10.1038/s41419-022-05339-x
Cited by 15
AI Summary: In this study, OriGene products including FLAG-human ATG5 (RC235557), non-tagged human ATG5 (SC128244), and MYC-DDK-PSMA7 (RC201169) plasmids were used to investigate the roles of autophagy and proteasome components in regulating PARK2 recruitment and mitophagy.
Snippet: neurin(OPTN)-EGFP (27052) and pmCherry-ATG5 (13095) plasmids were purchased from Addgene. FLAG-human ATG5 (RC235557), non-tagged human ATG5 (SC128244) and MYC-DDK-PSMA7 (RC201169) were purchased from Origene. GFP-LC3 construct also described [63]. GFP-PSMA7 vector was created by cloning using the MYC-DDK-PSMA7 construct into PEGFP-N3-empty vector. ATG5 KO lentiCRISPR V2 vector was created by cloni
FigureFigureFigure
Tumor heterogeneity in autophagy-dependent ferroptosis
Li, Liu, Xu et al
Autophagy (2021)
Cited by 247
AI Summary: OriGene contributed to this study by providing the Human ATG5 cDNA (SC128244), which was used to investigate the role of ATG5 in autophagy-dependent ferroptosis.
Snippet: Human ATG5 cDNA (SC128244) was obtained from OriGene Technologies Inc. Human ATG5-shRNA (sequence: CCGGCCTGAACAGAATCATCCTTAACTCGAGTTAAG­ GATGATTCTGTTCAGGTTTTTTG),
Impaired autophagy of an intracellular pathogen induced by a Crohn's disease associated ATG16L1 variant
Kuballa, Huett, Rioux et al
PLoS One (2008) 3 (10), e3391 DOI: 10.1371/journal.pone.0003391
Cited by 333
AI Summary: OriGene’s ATG16L1 cDNA clones were used in this study to express either the protective (ATG16L1*300T) or risk-associated (ATG16L1*300A) variants in human epithelial cells, enabling the authors to assess how these ATG16L1 forms affect autophagic capture of Salmonella.
FigureFigureFigure
Showing 1-3 of 3 papers.
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