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CTDSPL (NM_005808) Human Untagged Clone

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SKU
SC128039
CTDSPL (untagged)-Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 2
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CTDSPL
Synonyms C3orf8; HYA22; PSR1; RBSP3; SCP3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_005808, the custom clone sequence may differ by one or more nucleotides


ATGGACGGCCCGGCCATCATCACCCAGGTGACCAACCCCAAGGAGGACGAGGGCCGGTTGCCGGGCGCGG
GCGAGAAAGCCTCCCAGTGCAACGTCAGCTTAAAGAAGCAGAGGAGCCGCAGCATCCTTAGCTCCTTCTT
CTGCTGCTTCCGTGATTACAATGTGGAGGCCCCTCCACCCAGCAGCCCCAGTGTGCTTCCGCCACTGGTG
GAGGAGAATGGTGGGCTTCAGAAGCCACCAGCTAAGTACCTTCTTCCAGAGGTGACGGTGCTTGACTATG
GAAAGAAATGTGTGGTCATTGATTTAGATGAAACATTGGTGCACAGTTCGTTTAAGCCTATTAGTAATGC
TGATTTTATTGTTCCGGTTGAAATCGATGGAACTATACATCAGGTGTATGTGCTGAAGCGGCCACATGTG
GACGAGTTCCTCCAGAGGATGGGGCAGCTTTTTGAATGTGTGCTCTTTACTGCCAGCTTGGCCAAGTATG
CAGACCCTGTGGCTGACCTCCTAGACCGCTGGGGTGTGTTCCGGGCCCGGCTCTTCAGAGAATCATGTGT
TTTTCATCGTGGGAACTACGTGAAGGACCTGAGTCGCCTTGGGCGGGAGCTGAGCAAAGTGATCATTGTT
GACAATTCCCCTGCCTCATACATCTTCCATCCTGAGAATGCAGTGCCTGTGCAGTCCTGGTTCGATGACA
TGACGGACACGGAGCTGCTGGACCTCATCCCCTTCTTTGAGGGCCTGAGCCGGGAGGACGACGTGTACAG
CATGCTGCACAGACTCTGCAATAGGTAG

5' Read Nucleotide Sequence
>OriGene 5' read for NM_005808 unedited
ATTTTGTCATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCGGCCGCCGC
GCCGCGCACCCATGGACGGCCCGGCCATCATCACCCAGGTGACCAACCCCAAGGAGGACG
AGGGCCGGTTGCCGGGCGCGGGCGAGAAAGCCTCCCAGTGCAACGTCAGCTTAAAGAAGC
AGAGGAGCCGCAGCATCCTTAGCTCCTTCTTCTGCTGCTTCCGTGATTACAATGTGGAGG
CCCCTCCACCCAGCAGCCCCAGTGTGCTTCCGCCACTGGTGGAGGAGAATGGTGGGCTTC
AGAAGCCACCAGCTAAGTACCTTCTTCCAGAGGTGACGGTGCTTGACTATGGAAAGAAAT
GTGTGGTCATTGATTTAGATGAAACATTGGTGCACAGTTCGTTTAAGCCTATTAGTAATG
CTGATTTTATTGTTCCGGTTGAAATCGATGGAACTATACATCAGGTGTATGTGCTGAAGC
GGCCACATGTGGACGAGTTCCTCCAGAGGATGGGGCAGCTTTTTGAATGTGTGCTCTTTA
CTGCCAGCTTGGCCAAGTATGCAGACCCTGTGGCTGACCTCCTAGACCGTGGGGGTGTGT
TTCCGGGCCCGGCTCTTCAGAGATCATGTGTTTTTCATCGTGGGAACACGTGAAGGACCT
GAGTCGCCTTGGGCGGGAGCTGAGCAAAGTGATCATTTGTTGACAATTCCCCTGCCTCAT
ACATCTTCCATCCTGAGAATGCAGTGCTCTGTGCGNTCCCTGGGTTCCGATGACATGGAC
GGACACCGGNAGCTGCTGGACCTCATCCCCTTCTTTTTGAGGGCTCGAGCCGGNAGGAAC
CNACGTGTAACAGCATGCTGCACAGACTCTGCNATAGCTGGATACACGGGGGCCCGGTTG
GGGGAAATTTCCTCTGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005808 unedited
AAAGAACCTGTGGCTAGTTTTATTTTGGTTCGGCAGAGGAATACATTGCAAACCTTTTGC
ATTGAAATACTCGCCCCTGGTAAACGTGCACATTCCGATATGTAGCATTTCCTAAGTATT
GCTGGATTGTTTATCACATTCAAATGTCCAGCAGAAAATCCATGACTACCCANACACCAG
CATCTCCACTACCCGGGGTGGGGGAGGGTGTGTAAGTCTGCAGAGAAGCTCCTGGGAGAA
TGGAACAGGAACAGACATGATCAAAGTTACAGTACAGTAGGAACAGAGGCAGCCAGCCAA
TTTTGCACAGTTAACTGCAAGTACGTCAAGCCATTCACTCTTCTCGAACCCTGGCATGCC
AACTGTCCAGCACAGCACCTTTAAAAGCAGAGGCACAAACGTATGATTGTTTGACCATAG
GGCTGCGTGGTTCTGGATTTTTTATTAGAAAGCCATCTTTAAAAATACAGGGTTGTAAAA
TGTTGCCAGGTTACAGACCTACAGGTTTTTTTTTTTAACATATAAAGTTCAAATGCTGCC
CCCAGACAAGTCAGCGTCCTTAGGGCATCCTCCTGGTCCCCATAGACATCTCATCATAAT
TGCTCCACGACCATAGAACTTCCTCACCTTTTATTGTGCGCGGCTAGCTTCCCTTTATGC
AATTGTCAAACGCCTTATTTTCCGACCGCTGGTCGCTGGGCACCACTGTACTCACACCTT
TCCACTCCTCACCAATATTGTATTCCGCTTTCTTTTCCCCTCTTGGGAGTTGCCCCGGCC
CTTTGCATCATCTACCTTTGCTCCACACATTATTAATACCATATATCGCCCCGTATCTCT
ATCCCCCCCCGTCCCCCGCGCCGCACATTCGATTCACTTCACCACGCCCCCCCTAATAAT
TACCCTCACCGCC
Restriction Sites NotI-NotI |
ACCN NM_005808
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005808.2, NP_005799.2
RefSeq Size 4422 bp
RefSeq ORF 798 bp
Locus ID 10217
UniProt ID O15194
Cytogenetics 3p22.2
Protein Families Druggable Genome, Phosphatase
Summary Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells (By similarity). Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks an in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2) compared to isoform 1.
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Documents
Resources
"NM_005808" in other vectors (4)
SKU Description Size Price
RC214440 CTDSPL (Myc-DDK-tagged)-Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 2 10 ug
$300.00
RC214440L3 Lenti ORF clone of Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC214440L4 Lenti ORF clone of Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 2, mGFP tagged 10 ug
$600.00
RG214440 CTDSPL (tGFP-tagged) - Human CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like (CTDSPL), transcript variant 2 10 ug
$489.00 $500.00

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