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COL8A2 (NM_005202) Human Untagged Clone

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SKU
SC127921
COL8A2 (untagged)-Human collagen, type VIII, alpha 2 (COL8A2)
  $645.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol COL8A2
Synonyms FECD; FECD1; PPCD; PPCD2
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_005202, the custom clone sequence may differ by one or more nucleotides


ATGCTGGGGACTCTGACACCCCTGTCTTCGCTGCTGCTGCTGCTACTGGTGCTGGTGCTGGGGTGTGGGC
CGCGGGCGTCCTCTGGTGGCGGGGCCGGTGGGGCGGCGGGCTATGCCCCAGTGAAGTACATCCAGCCCAT
GCAGAAAGGACCTGTGGGACCGCCCTTCCGTGAGGGCAAAGGCCAGTACCTGGAAATGCCTCTACCGCTG
CTGCCGATGGACCTGAAGGGAGAGCCCGGCCCCCCTGGGAAGCCCGGGCCTCGGGGTCCCCCTGGCCCCC
CTGGCTTCCCAGGAAAACCAGGCATGGGAAAGCCAGGACTCCATGGGCAGCCTGGCCCTGCTGGGCCCCC
TGGCTTCTCCCGGATGGGCAAGGCTGGTCCCCCAGGGCTCCCTGGCAAGGTCGGGCCACCAGGGCAGCCG
GGGCTTCGGGGGGAGCCAGGAATACGAGGGGACCAGGGCCTCCGGGGACCCCCAGGACCCCCTGGCCTCC
CGGGCCCCTCAGGCATTACTATCCCTGGAAAACCAGGTGCCCAAGGGGTGCCAGGGCCCCCAGGATTCCA
GGGGGAACCAGGGCCCCAGGGGGAGCCTGGGCCCCCAGGTGATCGAGGCCTCAAGGGGGATAATGGAGTG
GGCCAGCCCGGGCTGCCTGGGGCCCCAGGGCAGGGGGGTGCCCCCGGCCCCCCCGGCCTCCCTGGTCCAG
CTGGCTTAGGCAAACCTGGTTTGGATGGGCTTCCTGGGGCCCCAGGAGACAAGGGTGAGTCTGGGCCTCC
TGGAGTTCCAGGCCCCAGGGGGGAGCCAGGAGCTGTGGGCCCAAAAGGACCTCCTGGAGTAGACGGTGTG
GGAGTCCCAGGGGCAGCAGGGTTGCCAGGACCACAGGGCCCATCAGGGGCCAAAGGGGAGCCAGGGACCC
GGGGCCCCCCTGGGCTGATAGGCCCCACTGGCTATGGGATGCCAGGACTGCCAGGCCCCAAGGGGGACAG
GGGCCCAGCTGGGGTCCCAGGACTCTTGGGGGACAGGGGTGAGCCAGGGGAGGATGGGGAGCCAGGGGAG
CAGGGCCCACAGGGTCTTGGGGGTCCCCCTGGACTTCCTGGGTCTGCAGGGCTTCCTGGCAGACGTGGGC
CCCCTGGGCCTAAGGGTGAGGCAGGGCCTGGAGGACCCCCAGGAGTGCCTGGCATTCGAGGTGACCAGGG
GCCTAGTGGCCTGGCTGGGAAACCAGGGGTCCCAGGTGAGAGGGGACTTCCTGGGGCCCATGGACCCCCT
GGACCAACTGGGCCCAAGGGTGAGCCGGGTTTCACGGGTCGCCCTGGAGGACCAGGGGTGGCAGGAGCCC
TGGGGCAGAAAGGTGACTTGGGGCTCCCTGGGCAGCCTGGCCTGAGGGGTCCCTCAGGAATCCCAGGACT
CCAGGGTCCAGCTGGCCCTATTGGGCCCCAAGGCCTGCCGGGCCTGAAGGGGGAACCAGGCCTGCCAGGG
CCCCCTGGAGAGGGGAGAGCAGGGGAACCTGGCACGGCTGGGCCCACGGGGCCCCCAGGGGTCCCTGGCT
CCCCTGGAATCACGGGCCCTCCGGGGCCTCCCGGGCCCCCGGGACCCCCTGGTGCCCCTGGGGCCTTCGA
TGAGACTGGCATCGCAGGCTTGCACCTGCCCAACGGCGGTGTGGAGGGTGCCGTGCTGGGCAAGGGGGGC
AAGCCACAGTTTGGGCTGGGCGAGCTGTCTGCCCATGCCACACCGGCCTTCACTGCGGTGCTCACCTCGC
CCTTCCCCGCCTCGGGCATGCCCGTGAAATTTGACCGGACTCTCTACAATGGCCACAGCGGCTACAACCC
AGCCACTGGCATCTTCACCTGCCCTGTGGGCGGCGTCTACTACTTTGCTTACCATGTGCACGTCAAGGGC
ACCAACGTGTGGGTGGCCCTGTACAAGAACAACGTGCCGGCCACCTATACCTACGATGAGTACAAGAAGG
GCTACCTGGACCAGGCATCTGGTGGGGCCGTGCTCCAGCTGCGGCCCAACGACCAGGTCTGGGTGCAGAT
GCCGTCGGACCAGGCCAACGGCCTCTACTCCACGGAGTACATCCACTCCTCCTTTTCAGGATTCTTGCTC
TGCCCCACATAA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_005202 unedited
AGACTCATATAGGCGGCCGCGAAATTCCCCTTGAAGCCATGCTGGGGACTCTGACACCCC
TGTCTTCGCTGCTGCTGCTGCTACTGGTGACTGTGCTGGGGTGTGGGCCACGGGCGTACT
TTGGTTGGCGGGCCCGGTGGGGCAACCGGCATTTCCCCCATGAAATAATTCCCCCCCCTG
CCAAAAGGAACCTTGTGGACCCCCCTTTTTTTGGGGCAAAAGCACCAACCTCGGAAAAAA
CCCCTTCCCCCCTCTTCCAAAGAAAACACAAAAGAAAAAAAACCCCCTCCCCTTGAAAAA
ACCCGCCCCTCGGGTTTTCCCTTCCCCCCCTGTTTTTTTTTAAAAAAAAAAACTGGGGAA
AAAAAAGAAAATTTTTGGGGNGCGCCGCTCTGTGGGCCCCCCGTTTTTTTTCTTTTTGGG
GGGAGGTGGGCCCCCCCGCCCCCCCCCCGGGGGGGGGGGGAAAAAAAAAAAACAAGTGTT
TTGTGGTGGGGAGAGGNAAAAAAAAAAAAAGAAAAAAAAGNGTTGTTTTGGGTNCAAAAA
AAAAAAACAAAAAAAAAAAGCGGCCGGGGNNNGGCCCCCTTTTTTTTTTTTTTTAAAAAA
AAAACAAAAAAAGAGGGGGGGGGGGCGGGCGCGCGGTTTTTTTTTTCTTATAAAAAAAGG
AACCCCCGGGGGGGGGGGCGGGGCCACTTTTTTTATTTTATCCTATTTTNGGGAAAAAAA
AAAAGAAGAATCAACAAAAAACCCCCCCCTCCCGCCGCCCNNACAGGGGGGGGGGGAATA
AAGGGGATTTTNACAATATAAATANAACACAAAAATATAAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_005202 unedited
NGGGCCCCATCGGAGATTGGCAACTTNCCAGGNCCAGNANAGCACTGGGNGAGGGTCACA
GGGATGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG
ACAAGCTTGATATCGGTACCAGATCTGAATTCGCCCTTGCCGCCTCTGTTCAGCTTTTGT
TTTTTTTTCCAGGAGGTTCTTTGTAATTGAAAAGGTCGCTCTACCACTAAAGGGGAGGAG
GCCAGGGCAGCAGGACACCCCCCGCGGGTTATGTGGGGCAGAGCAAGAATCCTGAAAAGG
AGGAGTGGATGTACTCCGTGGAGTAGAGGCCGTTGGCCTGGTCCGACGGCATCTGCACCC
AGAACCTGGTCGTTGGGCCGCAGCTGGAGCACGGCCCCACCAGATGCCTGGTCCAGGTAG
CCCTTCTTGTACTCATCGTAGGTATAGGTGGCCGGCACGTTGTTCTTGTACAGGGCCACC
CACACGTTGGTGCCCTTGACGTGCACATGGTAAGCAAAGTAGTAGACGCCGCCCACAGGG
CAGGTGAAGATGCCAGTGGCTGGGTTGTAGCCGCTGTGGCCATTGTAGAGAGTCCGGTCA
AATTTCACGGGCATGCCCGAGGCGGGGAAGGGCGAGGTGAGCACCGCAGTGAAGGCCGGT
GTGGCATGGGCAGACAGCTCGCCCAGCCCAAACTGTGGCTTGCCCCCCTTGCCCAGCACG
GCACCCTCCACACCGCCGTTGGGCAGGTGCAAGCCTGCGATGCCAGTCTNATCGAAAGCC
CCAGGGGCACCANGGGGGTCCCGGGGGCCCCGGGAAGCCCCGGAGGGCCGTGATNCCCCA
GGGGGANCNNGNNCNNCNGGGGGGCCCCNNGGNNCCCNNNNNGCCNNNNNNNCNNNNNNN
NNNCNNNNNNGGGGT
Restriction Sites Please inquire |
ACCN NM_005202
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005202.1, NP_005193.1
RefSeq Size 4443 bp
RefSeq ORF 2112 bp
Locus ID 1296
UniProt ID P25067
Cytogenetics 1p34.3
Protein Families Druggable Genome
Summary This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jun 2014
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_005202" in other vectors (4)
SKU Description Size Price
RC216003 COL8A2 (Myc-DDK-tagged)-Human collagen, type VIII, alpha 2 (COL8A2) 10 ug
$644.00
RC216003L3 Lenti ORF clone of Human collagen, type VIII, alpha 2 (COL8A2), Myc-DDK-tagged 10 ug
$944.00
RC216003L4 Lenti ORF clone of Human collagen, type VIII, alpha 2 (COL8A2), mGFP tagged 10 ug
$944.00
RG216003 COL8A2 (tGFP-tagged) - Human collagen, type VIII, alpha 2 (COL8A2) 10 ug
$789.00 $844.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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