EGF (NM_001963) Human Untagged Clone
$1,051.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | EGF |
| Synonyms | HOMG4; URG |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_001963 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001963.2, NP_001954.1 |
| RefSeq Size | 4877 bp |
| RefSeq ORF | 3624 bp |
| Locus ID | 1950 |
| UniProt ID | P01133 |
| Cytogenetics | 4q25 |
| Domains | EGF, EGF_CA, ldl_recept_b |
| Protein Families | Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane |
| Protein Pathways | Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton |
| Summary | This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. provided by RefSeq, Jan 2016 Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
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Resources
"NM_001963" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210817 | EGF (Myc-DDK-tagged)-Human epidermal growth factor (EGF), transcript variant 1 | 10 ug |
$1,051.00
|
|
| RC210817L1 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,351.00
|
|
| RC210817L2 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, mGFP tagged | 10 ug |
$1,351.00
|
|
| RC210817L3 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,351.00
|
|
| RC210817L4 | Lenti ORF clone of Human epidermal growth factor (EGF), transcript variant 1, mGFP tagged | 10 ug |
$1,351.00
|
|
| RG210817 | EGF (tGFP-tagged) - Human epidermal growth factor (EGF), transcript variant 1 | 10 ug |
$789.00
$1,251.00
|
|
Citations
Soluble, but not precursor EGF induces intracrine signaling of the EGFR
Bilakovics, Volkó, Jeppesen et al
bioRxiv (2025)
Bilakovics, Volkó, Jeppesen et al
bioRxiv (2025)
AI Summary: OriGene’s preEGF cDNA (SC127840) was used to generate tagged sEGF/preEGF ligands for investigating their roles in EGFR intracrine signaling in this study.
Snippet: The cDNA encoding preEGF (OriGene, SC127840) was used to generate the N493 terminally mScarlet3-tagged sEGF/preEGF ligands in two steps. First, the signal peptide 494 sequence was inserted with NheI/AgeI restriction sites to the N-terminus of the mScarlet-3-C3 495 vector. In the second step, the remaining coding sequence of the sEGF/preEGF ligand was
Autocrine EGF and TGF-α promote primary and acquired resistance to ALK/c-Met kinase inhibitors in non-small-cell lung cancer
Wang, Zhang, Chen et al
Pharmacol Res Perspect (2023) 11 (1), e01047 DOI: 10.1002/prp2.1047
Wang, Zhang, Chen et al
Pharmacol Res Perspect (2023) 11 (1), e01047 DOI: 10.1002/prp2.1047
Cited by 9
AI Summary: OriGene products EGF cDNA (SC127840) and TGF-α cDNA (SC311226) were used in this study to overexpress EGF and TGF-α in NSCLC cell lines, enabling the researchers to demonstrate that increased autocrine production of these growth factors induces resistance to ALK/c-Met kinase inhibitors.
Snippet: wth factor concentration to cell protein concentration. 2.5 DNA plasmid construction, virus production, and infection DNA plasmids EGF cDNA (SC127840) and TGF‐α cDNA (SC311226) were obtained from Origene (Rockville, MD). The retroviral constructs pBABE‐EGF and pBABE‐TGF‐α were constructed using ClonExpress II One Step Cloning Kit (Vazyme, China) and subsequently subcloned into the pBABE
EGFR ligands exhibit functional differences in models of paracrine and autocrine signaling
Wilson, Mill, Lambert et al
Growth Factors (2012) 30 (2), 107-16 DOI: 10.3109/08977194.2011.649918
Wilson, Mill, Lambert et al
Growth Factors (2012) 30 (2), 107-16 DOI: 10.3109/08977194.2011.649918
Cited by 136
Showing 1-3 of 3 papers.
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