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RBFOX2 (NM_014309) Human Untagged Clone

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SKU
SC127805
RBFOX2 (untagged)-Human RNA binding protein, fox-1 homolog (C. elegans) 2 (RBFOX2), transcript variant 2
  $457.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RBFOX2
Synonyms dJ106I20.3; Fox-2; FOX2; fxh; HNRBP2; HRNBP2; RBM9; RTA
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_014309, the custom clone sequence may differ by one or more nucleotides


ATGGAGAAAAAGAAAATGGTAACTCAGGGTAACCAGGAGCCGACAACAACTCCTGACGCAATGGTTCAGC
CTTTTACTACCATCCCATTTCCACCACCTCCGCAGAATGGAATTCCCACAGAGTATGGGGTGCCACACAC
TCAAGACTATGCCGGCCAGACCGGTGAGCATAACCTGACACTCTACGGAAGTACGCAAGCCCACGGGGAG
CAGAGCAGCAACTCACCCAGCACACAAAATGGATCTCTTACGCAGACAGAAGGTGGAGCACAGACAGACG
GCCAGCAGTCACAGACACAAAGTAGTGAAAATTCAGAGAGTAAATCTACCCCGAAACGGCTGCATGTCTC
TAATATTCCTTTCCGCTTCCGGGACCCTGACCTCCGGCAGATGTTTGGGCAGTTTGGCAAAATCCTAGAT
GTAGAAATAATCTTTAATGAACGTGGCTCTAAGGGATTCGGGTTCGTAACTTTCGAGAATAGTGCTGATG
CAGACAGGGCCAGGGAGAAATTACACGGCACCGTGGTAGAGGGCCGTAAAATCGAGGTGAATAATGCTAC
AGCACGTGTAATGACCAATAAGAAGATGGTCACACCATATGCAAATGGTTGGAAATTAAGCCCAGTAGTT
GGAGCTGTATATGGTCCGGAGTTATATGCAGCATCCAGCTTTCAAGCAGATGTGTCCCTAGGCAATGATG
CAGCAGTGCCCCTATCAGGAAGAGGGGGTATCAACACTTACATTCCTTTAATCATTCCTGGCTTCCCTTA
CCCTACTGCAGCCACCACGGCAGCCGCTTTCAGAGGAGCCCATTTGAGGGGCAGAGGGCGGACAGTATAT
GGTGCAGTCCGAGCGGTACCTCCAACAGCCATCCCCGCCTATCCAGGGGTGGATATGCAGCCTACAGATA
TGCACAGCCTGCTACTGCAACCGCAGCCACCGCTGCTGCAGCCGCTGCAGCCGCTTACAGTGACGGTTAT
GGCAGGGTGTACACAGCCGACCCCTACCATGCCCTTGCCCCTGCCGCTAGCTATGGAGTTGGCGCTGTGG
CGAGTTTATACCGAGGTGGCTACAGCCGATTTGCCCCCTACTGAAGTGACGTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_014309 unedited
CTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAAGAAACATTAGAAAGAAAAAGGAAG
GAAAACGGTATAAAGAGAGATCAATTACCCACCCTTAAATAGCTAGATTGGGGGGGGAGG
GGGGTGGAAAAGAAAGCTGTGGAGGTGTGCCCCAGCACGGCTGCTTTGAAAGGTTTATCA
TCTATCCGTTTGGTTTATGGAGAAAAAGAAAATGGTAACTCAGGGTAACCAGGAGCCGAC
AACAACTCCTGACGCAATGGTTCAGCCTTTTACTACCATCCCATTTCCACCACCTCCGCA
GAATGGAATTCCCACAGAGTATGGGGTGCCACACACTCAAGACTATGCCGGCCAGACCGG
TGAGCATAACCTGACACTCTACGGAAGTACGCAAGCCCACGGGGAGCAGAGCAGCAACTC
ACCCAGCACACAAAATGGATCTCTTACGACAGAAGGTGGAGCACAGACAGACGGCCAGCA
GTCACAGACACAAAGTAGTGAAAATTCAGAGAGTAAATCTACCCCGAAACGGCTGCATGT
CTCTAATATTCCTTTCCGCTCCCGGGACCCTGACCTCCGGCAGATGTTTGGGGCAGTTTG
GCAAAATCCTAGATGTAGAAATAATCTTTATGGACGTGGCTTTAGGAATTCCGGGTTCTA
ACTTTTCAAAATAGGGGTGATGCCAACCGGGCCCGGAAAAAATACACGCGCCCCGGGTAA
AGGGCCCCTAAAATCCGGGGGGAATAGGCTCCCCAACCCTTTATAACCCCATAAAAAAAG
GGCCCCCCCCTTTCCCAAAGGTTGGAAAATAACCCCCCTATTTGGGGACTTTTTA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_014309 unedited
GGTTGTTTTTTCTTCTTTAACTTGCTATANAGTTCCTCTACTAGTAAATATTGCAATAGC
CAGGCCTCATGAACTGGGGGGCTGTCCCATTTGCAGGGGTCTCACGTCACTTCAGTAGGG
GGCAAATCGGCTGTAGCCACCTCGGTATAAACTCGCCACAGCGCCAACTCCATAGCTAGC
GGCAGGGGCAAGGGCATGGTAGGGGTCGGCTGTGTACACCCTGCCATAACCGTCACTGTA
AGCGGCTGCAGCGGCTGCAGCAGCGGTGGCTGCGGTTGCAGTAGCAGGCTGTGCATATCT
GTAGGCTGCATATCCCACCCCTGGTTGAACTGAAAACATTTTGCCAAATTTTATTTAAAG
GGGACCCCCCCCAAAAACCCCCCTGGGGGAAAACCCCCCCTTTAATATGGGGGGGGTTTT
TTTTTTGAAGAGAACACCCCCNAAACCACAAAAAAAATATTGTGCCGCCCCCCCCCCCCC
CCCCAAAAGGGGGNCCCCTTAAAAAAAAAAAACATTCGGGGGGGGGGTGGGGGGNNGGGG
GGGGGGGGGCCCCCCCCCCCCCCCCCAGGGAAGGGGNAAAATAAAGGAGGGGGGGGCCCG
GGTCCCCCCCCCCTCCCCTTCGCCGGGGGGGGGGGGGGTGGGTTCCTCTTCTCCCCCCCC
CATATTACCACCCGCCTGGGGGGCGCGGTTTCCCCTTTCTTCCCCCACCCTACCACTCCC
CACCCCCTTCCCCCTNGTTTCCTCTCCACATTCNTTCCGCCCCCCNNCCCCGGTCGTNNC
TCTTTCTTCCCACCCTCCCCNACCNATCCACCCACCNATACCAATTTTTTTCTCCCCCCC
CCTTCCCTCCCCCCNNCGCCCTGCTGTGTCCGCTCCNCCCCTCCTTTTNNTCCCCTCCCT
CCTTCCTTCCTTTCTCCTCCACCCCCTCCCCCCCTTTCCATTTTCTCCCCCCCCCCCTTT
CCCCGCCTTTTACTTCCCCTCCCCGCCCGCGCTGA
Restriction Sites NotI-NotI |
ACCN NM_014309
Insert Size 1530 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014309.1, NP_055124.1
RefSeq Size 1876 bp
RefSeq ORF 1104 bp
Locus ID 23543
UniProt ID O43251
Cytogenetics 22q12.3
Domains RRM
Protein Families Druggable Genome, Transcription Factors
Summary This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
Transcript Variant: This variant (2) uses two alternate in-frame splice sites in the coding region, and lacks an alternate exon in the 3' coding region that results in a frameshift, compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
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Documents
Resources
"NM_014309" in other vectors (6)
SKU Description Size Price
RC222904 RBFOX2 (Myc-DDK-tagged)-Human RNA binding protein, fox-1 homolog (C. elegans) 2 (RBFOX2), transcript variant 2 10 ug
$289.00 $457.00
RC222904L1 Lenti-ORF clone of RBFOX2 (Myc-DDK-tagged)-Human RNA binding protein, fox-1 homolog (C. elegans) 2 (RBFOX2), transcript variant 2 10 ug
$757.00
RC222904L2 Lenti-ORF clone of RBFOX2 (mGFP-tagged)-Human RNA binding protein, fox-1 homolog (C. elegans) 2 (RBFOX2), transcript variant 2 10 ug
$757.00
RC222904L3 Lenti-ORF clone of RBFOX2 (Myc-DDK-tagged)-Human RNA binding protein, fox-1 homolog (C. elegans) 2 (RBFOX2), transcript variant 2 10 ug
$757.00
RC222904L4 Lenti-ORF clone of RBFOX2 (mGFP-tagged)-Human RNA binding protein, fox-1 homolog (C. elegans) 2 (RBFOX2), transcript variant 2 10 ug
$757.00
RG222904 RBFOX2 (tGFP-tagged) - Human RNA binding protein, fox-1 homolog (C. elegans) 2 (RBFOX2), transcript variant 2 10 ug
$657.00

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