DPM2 (NM_003863) Human Untagged Clone

SKU SC127744

DPM2 (untagged)-Human dolichyl-phosphate mannosyltransferase polypeptide 2, regulatory subunit (DPM2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: DPM2
• Synonyms: CDG1U
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127744 sequence for NM_003863 edited (data generated by NextGen Sequencing); ATGGCCACGGGGACAGACCAGGTGGTGGGACTCGGCCTCGTCGCCGTTAGCCTGATCATC; TTCACCTACTACACCGCCTGGGTGATTCTCTTGCCATTCATCGACAGTCAGCATGTCATC; CACAAGTATTTCCTGCCCCGAGCCTATGCTGTCGCCATCCCACTGGCTGCAGGCCTCCTG; CTGCTCCTGTTTGTGGGACTGTTCATCTCCTACGTGATGCTGAAGAGCAAGAGAGTGACC; AAGAAGGCTCAGTGA; ; Clone variation with respect to NM_003863.3; 213 t=>c;227 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003863 unedited; CGTCCAGAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCG; CGGGNGAAATGGCCACGGGGACAGACCAGGTGGTGGGACTCGGCCTCGTCGCCGTTAGCC; TGATCATCTTCACCTACTACACCGCCTGGGTGATTCTCTTGCCATTCATCGACAGTCAGC; ATGTCATCCACAAGTATTTCCTGCCCCGAGCCTATGCTGTCGCCATCCCACTGGCTGCAG; GCCTCCTGCTGCTCCTGTTTGTGGGACTGTTCATCTCCTACGTGATGCTGAAGAGCAAGA; GAGTGACCAAGAAGGCTCAGTGAAGGTCCCGCAGGGATGAGGCTGCCAGCCCCTTCTCTG; CTTCCCCTCCAGCACAGGGACCAAGTGGGGGAGCCTGCAGAACCTGTCCAGGCACAGTGG; CTCCTCAAGCCTGCCTGTCCTGCAGAGTCCCCATGGCATGGAGCTTACACCTGACTGACT; GGAGCCCCCTCCCCGACTCCCACTTCCAGAAGCTAGGAGGGAGGGATACCTGGAAGACTC; CGGTCACCTCCTTCTTGCTCANGGCCTAAAAGATGCTGGTCCTCCCAACCTCACTCTCAG; ACTCCCTGCCACCTTTTCCCCTGNGTTCTGCCGTCTTGCCTCACTTCCCCTCCTGTCACA; TGCTGACGTTGGACTTAGCANGTTCTAAGGCCACATGTGTGACCTCTCTGACTTCTCTTC; CTNCCCAAAGCAGCTTTCCTTACCCTGAACAGCCCCAGACCCACAAAGCCTTCTGACCCT; GAAAGCTGGGGAAGACTGACAGACCCCAGAACAGCCCTGGGCTCAAGCAGCCACCCCGGC; CGCTGACGACTGGACTCTCTCACGGAGCCCAGCCCAAACCCCAAGCTGGCCCGTGGGGAC; ACTAACCATAAACCGATGTGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_003863
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003863.1, NP_003854.1
• RefSeq Size: 291 bp
• RefSeq ORF: 255 bp
• Locus ID: 8818
• UniProt ID: O94777
• Cytogenetics: 9q34.11
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways, N-Glycan biosynthesis
• Summary: Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. The protein encoded by this gene is a hydrophobic protein that contains 2 predicted transmembrane domains and a putative ER localization signal near the C terminus. This protein associates with DPM1 in vivo and is required for the ER localization and stable expression of DPM1 and also enhances the binding of dolichol-phosphate to DPM1. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer isoform (1).