VPS4B (NM_004869) Human Untagged Clone

SKU SC127708

VPS4B (untagged)-Human vacuolar protein sorting 4 homolog B (S. cerevisiae) (VPS4B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: VPS4B
• Synonyms: MIG1; SKD1; SKD1B; VPS4-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127708 sequence for NM_004869 edited (data generated by NextGen Sequencing); ATGTCATCCACTTCGCCCAACCTCCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCAA; GAAGACAAGGCTGGGAACTACGAAGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTAT; TTTCTTCATGTCGTTAAATATGAAGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGCA; AAGTGTACAGAATATCTTGATAGAGCAGAAAAACTAAAGGAGTACCTGAAAAATAAAGAG; AAAAAAGCACAGAAGCCAGTGAAAGAAGGACAGCCGAGTCCAGCAGATGAGAAGGGGAAT; GACAGTGATGGGGAAGGAGAATCTGATGATCCTGAAAAAAAGAAACTACAGAATCAACTT; CAAGGTGCCATTGTTATAGAACGACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGAA; GGAGCCAAAGAAGCACTGAAAGAGGCTGTGATACTGCCTATTAAATTTCCTCATCTTTTT; ACAGGCAAGAGAACACCTTGGAGGGGAATCCTATTATTTGGGCCGCCTGGAACAGGAAAG; TCCTACTTAGCCAAAGCTGTAGCAACAGAAGCCAACAACTCAACATTTTTTTCAATATCT; TCCTCTGATCTTGTTTCTAAGTGGCTAGGTGAAAGTGAAAAACTGGTTAAGAATTTATTC; CAACTTGCCAGAGAGAACAAGCCCTCCATTATCTTCATTGATGAAATTGATTCTCTCTGT; GGTTCAAGAAGTGAAAATGAAAGTGAAGCCGCACGTAGAATTAAGACGGAGTTCCTAGTG; CAAATGCAAGGGGTTGGTGTAGACAATGATGGAATTTTGGTTCTGGGAGCTACAAATATA; CCCTGGGTTCTGGATTCTGCCATTAGGCGAAGATTTGAGAAACGAATTTATATTCCCTTG; CCGGAACCCCATGCCCGAGCAGCAATGTTTAAACTGCACCTAGGGACCACTCAGAACAGT; CTCACGGAAGCAGACTTTCGGGAACTTGGGAGGAAAACAGATGGTTATTCAGGGGCAGAT; ATAAGTATCATTGTACGTGATGCCCTTATGCAGCCTGTTAGGAAAGTACAGTCAGCTACT; CATTTTAAAAAGGTTCGCGGACCTTCCCGAGCTGATCCTAACCATCTTGTAGATGATCTG; CTAACACCTTGCTCTCCAGGTGACCCTGGTGCCATTGAAATGACATGGATGGATGTCCCT; GGAGATAAACTTTTGGAGCCAGTTGTTTCCATGTCGGATATGTTGCGGTCACTATCTAAC; ACAAAACCTACAGTCAATGAACATGACTTGTTGAAATTAAAGAAGTTTACAGAAGATTTT; GGTCAAGAAGGCTAA; ; Clone variation with respect to NM_004869.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004869 unedited; CGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGTCCGAGAACCACCGGCCTC; CCCAGTTTGAGGGCTGTTACCCCGTGCGCGCTTCGACGTTGCTGCTGTTGGCTCTCCTCG; CCCCTCGTTCCCTTGGGAACCGCCTGGGAACTCCGCCATGTCATCCACTTCGCCCAACCT; CCAGAAAGCGATAGATCTGGCTAGCAAAGCAGCGCAAGAAGACAAGGCTGGGAACTACGA; AGAAGCCCTTCAGCTCTATCAGCATGCTGTGCAGTATTTTCTTCATGTCGTTAAATATGA; AGCACAGGGTGATAAAGCCAAGCAAAGTATCAGGGCAAAGTGTACAGAATATCTTGATAG; AGCAGAAAAACTAAAGGAGTACCTGAAAAATAAAGAGAAAAAAGCACAGAAGCCAGTGAA; AGAAGGACAGCCGAGTCCAGCAGATGAGAAGGGGAATGACAGTGATGGGGAAGGAGAATC; TGATGATCCTGANAAAAAGAAACTACAGAATCAACTTCAAGGTGCCATTGTTATAGAACG; ACCAAATGTGAAATGGAGTGACGTTGCTGGACTTGAAGGAGCCAAAGAAGCACTGGAAGA; GGCTGTGATACTGCCTATTAATTNCCTCATCTTTNTACAGGGCAGAGAACACCTTGGNAG; GGGATCCCNTATATTTGGCCGCCTGGAACAGGNAAGTCCTACTTAGCCAAAGCTGTAGCA; ACAGAAGCCAANCCACTCACATTTTTTACAATATCTCCTCTGGATCTGNTNCTAAGTGGC; TAGTGAAAGTGAAAAACTGGGTAAGAANTTATTNCNNACTGCCAGAGAGAACCAGCCCTC; CATTATCTNCATTGATGAAAANTGATCTTCCTGTGGNTCAAGAAAGTGAAATGAAAGTGA; GCCCCACGTANAATTAGACGGAGTCCTATGCCATGCAGGGGTNGNGTANCATGATGAATT; TGGNTCTGGACTACAATTACC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004869 unedited; GCGGCCGCGGGGGCAGNCCGCANACTCTCCCNNNNGGTTTTTACTTGNNACGCGGCCGCT; TTTNANATCGNGTTTTTTTTTTTTTTTTTTTTTAGGGTATATCAATTTACTTCACATAAG; AGCATAATTCAGGCCTTTTCCATTTAATAACAAAAACAATCTGTACAGAAAACCCAAAGG; CAACCACATAGCATATGTAAAATGTGCAAATACACTTTAAAATGCAAGTTATTCTATAGC; ATTTGCAAGATAGAATTTCACTGTAATTAGGGAATCTAGTTCATCCTAACTTAATAGTCT; TTTGCATGTATAGACAATGCAATTCTACAAGGCACAACTCAGCGTTGATGCTAAAGTATG; AAACACATCCTCAGATTATTTATTTGAAAATATTAAAATAGCATCATGTTTATTATTTTT; TAATGAGTCATGAGCTCATTTCTAAAGCTTCATAAAGCATTACACTGATAACATATGTGT; GGTCAGGACAAACTGTTCCCTGAACTTAAAAGGTGAAGGACAAGACCCCATATTATTATC; CTGTATTAAAAAAGGAAATATACATATATGTACACAGACACCCCATATCACAGACAAGAA; ACTTCCCATAATTCAAAGGGAGACCATTTCCTTATTAGCAAAGGTGCGCATTACAGTATT; TCATGACAGTTAAAAATTACACACCTACCACCTGTTTTTGGTCAATCTTGCTAAAAAAGA; ACTGAAATAGACAATTTCTTCTTTAAGGTAAAGACAATGTCTAATTTAGAAAATCTTCCT; CTTGAGATGCACAGTGAAAGGTATCAGNTAATTAATATTAAAGCTTACCGTTTTTGTTTT; TTTTGAGACGAGTTTTATTCAGTCGCCAAGCTTGATGCAATGGGCTTCTTGGGCCCAAGC; TGGATGCAATACGCGATTCAACTACTGGAACTCTTGCTTCAGTTCAGTGATTCTCCGCT;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004869
• Insert Size: 3160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004869.2, NP_004860.2
• RefSeq Size: 3337 bp
• RefSeq ORF: 1335 bp
• Locus ID: 9525
• UniProt ID: O75351
• Cytogenetics: 18q21.33
• Domains: AAA, MIT
• Protein Families: Transcription Factors
• Protein Pathways: Endocytosis
• Summary: The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq, Jul 2008]