RAP2B (NM_002886) Human Untagged Clone

SKU SC127520

RAP2B (untagged)-Human RAP2B, member of RAS oncogene family (RAP2B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: RAP2B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127520 sequence for NM_002886 edited (data generated by NextGen Sequencing); ATGAGAGAGTACAAAGTGGTGGTGCTGGGCTCGGGCGGCGTGGGCAAGTCCGCGCTCACC; GTGCAGTTCGTGACGGGCTCCTTCATCGAGAAGTACGACCCGACCATCGAAGACTTTTAC; CGCAAGGAGATTGAGGTGGACTCGTCGCCGTCGGTGCTGGAGATCCTGGATACGGCGGGC; ACCGAGCAGTTCGCGTCCATGCGGGACCTGTACATCAAGAACGGCCAGGGCTTCATCCTG; GTCTACAGCCTCGTCAACCAGCAGAGCTTCCAGGACATCAAGCCCATGCGGGACCAGATC; ATCCGCGTGAAGCGGTACGAGCGCGTGCCCATGATCCTGGTGGGCAACAAGGTGGACCTG; GAGGGTGAGCGCGAGGTCTCGTACGGGGAGGGCAAGGCCCTGGCTGAGGAGTGGAGCTGC; CCCTTCATGGAGACGTCGGCCAAAAACAAAGCCTCGGTAGACGAGCTATTTGCCGAGATC; GTGCGGCAGATGAACTACGCGGCGCAGCCCAACGGCGATGAGGGCTGCTGCTCGGCCTGC; GTGATCCTCTGA; ; Clone variation with respect to NM_002886.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002886 unedited; NNGGGCGTCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGCCGCGG; CGGTGGGCGCACGTTCCGCGGGGACTCATGCCACGCGCGTCCCGGCCCGACGCGCAATTA; GCAGCCACCTCCGCAGCCCGCCGCCACCGCCTCCCTGCCCTCCCGGGCTGCCGCAGCTAG; GAGCTCCAGCCGTCGCCTCGCGCAGGCTGCGGGCATTGTCCTCTCGGTTCGCCGCCCGGG; CTGCTACTGCCGCCGCGGACTGCTGCGGGGCCCGGACCCGCACCCCAGGGATACGCTGCC; GCCGCCGCCGGCCGGCCCGGCGCCCGGCCTCCGTTCGGTGGTTTCCGCCCTGCGTTCTCT; GGGTTGCTCTCTCCTGGGTTTTTCCTGCGTAGCTGAGGAAGGGGAAGAGAAGTCCAGCCG; CCAAGCCCAGCCTTCCCCGGCGCGCAGCCCCGACGGGGCCGCGGCAGGCGCGGCGAGAGC; GCTGACGGAGCCATGAGAGAGTACANAGTGGTGGTGCTGGGCTCGGGCGGCGTGGGCAAG; TCCGCGCTCACCGTGCAGTTCGTGACGGGCTCCTTCATCGAGAAGTACGACCCGACCATC; GAAGACTTTTACCGCAGGGAGATTTGAGTGGACTCGTCGCCGTCNGTGCTGGAGATCCTG; GATACGGCGGGCACGGAGCAGTTCGCGTCCCTGCGGGGACCTGTACATCAAGAACGGCCA; GGGCTTCATCCTGGTCTACAGCCTCGTCAACCAGCAAAGCTTCCAGGACATTAAGCCCAT; GCGGGACCAGATCATTCGCGTGAAGCGGTACCAGCGCTTGCCCTTGATCCTGGTGGGCAC; CAGGTTGAACTTGGAGGGTGGACCCCAGGCTTCCTACGGGGAGGCCAAGGCCCCGGCCTA; AGAATGGAACC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002886 unedited; GGGGGGCCNNNNCCCNTNTTTNCNNNNNNNNNTTATCNTAGNNCCNCGGNCCGCATACNA; NGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTCTTTAAAAAGACATTTGT; ATTAAAAGTCTTAAAATGAAGAAGAAATGATTAAACGCATGAAATTAAAAATTACAATTA; GTTAGAATTGGTACATCACAACAGTAAAATTTTGCTCTTTGCTTCTGGAGGAACACCCTA; CAACAGGTAATTAAAAAATTTAAAAATAATATGAAAGGGGAGCTAAATACCTGAAAATTT; AAACAAAATGAAGCAAAAAAAAGGGAAACAAATTCATTGCAAGAGATGGAAATTACATTT; TAAAGGCTGAAAATAATAAAGAAATTCACTAAACAACAGGCCCTATTGTCCTGGCTCCTT; CAGAAAGATTACTGAAAGGAAACTGGGAAGTGGTTTGGGGAATAGCAGATTCTGGGATAT; GGTGGGAAGTTCACAGGTTGGCACCGGCTGGAACAGCTGAGTTTTGAAGGCACTCTGCAG; ACACAGGGGTGCTGGGGGCCCCTGATCACATTTACAAAGTCTTTGTACTGTATTTTTTAA; AGCTAGTCATTAAAATCGGTTACTGCCCACCAAAAAACAAAAACAAAGGCTAAACTTNAA; NCACACACCCCCNAAACACATACACACACCCCCCTTGTACACACGAAAATGTGCAAATCT; GTGACATTTTTTTTACAGCTGATAAAAAAAAAAATACTACCCATTTTCCCCAGACTTCCC; TTTGATACACGAGGTAAACAAAATAGATTTTTTTTCCCCAATTCAAGCGGACCCTTCTGG; CCCCCCAACTTGNTTGGCCATCAAGGGTAACAAGTCCAGTTCCAATGGGCAATCTTTAAA; ATTGGATCACATGGACCANGAATTCCAATTTGCATAAACATNCCTAAAATACCAATCAGT; CTTGGGGGTTGCCCCTCTCAGACTGGCAAAAAGGAAACCCCATCTAAATTTCCACTACTT; CTGAANAAGNNGNAAANNNAGTTTTNCTTTCTCCTGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_002886
• Insert Size: 2440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002886.2, NP_002877.2
• RefSeq Size: 6237 bp
• RefSeq ORF: 552 bp
• Locus ID: 5912
• UniProt ID: P61225
• Cytogenetics: 3q25.2
• Domains: RAB, ras, RAS, RHO
• Protein Families: Druggable Genome
• Summary: This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated. [provided by RefSeq, Jul 2008]