GOT2 (NM_002080) Human Untagged Clone
SKU
SC127269
GOT2 (untagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | GOT2 |
| Synonyms | DEE82; KAT4; KATIV; KYAT4; mitAAT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | NotI-NotI | |
| ACCN | NM_002080 |
| Insert Size | 2410 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_002080.2, NP_002071.2 |
| RefSeq Size | 2473 bp |
| RefSeq ORF | 1293 bp |
| Locus ID | 2806 |
| UniProt ID | P00505 |
| Cytogenetics | 16q21 |
| Domains | aminotran_1_2 |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Alanine, Arginine and proline metabolism, aspartate and glutamate metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, Phenylalanine metabolism, tyrosine and tryptophan biosynthesis, Tyrosine metabolism |
| Summary | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2013 Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_002080" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC201826 | GOT2 (Myc-DDK-tagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein | 10 ug |
$686.00
|
|
| RC201826L3 | Lenti-ORF clone of GOT2 (Myc-DDK-tagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein | 10 ug |
$986.00
|
|
| RC201826L4 | Lenti-ORF clone of GOT2 (mGFP-tagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein | 10 ug |
$986.00
|
|
| RG201826 | GOT2 (tGFP-tagged) - Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein | 10 ug |
$886.00
|
|
| SC320593 | GOT2 (untagged)-Human glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), nuclear gene encoding mitochondrial protein | 10 ug |
$686.00
|
|
Citations
Targeted exome sequencing of Krebs cycle genes reveals candidate cancer predisposing mutations in pheochromocytomas and paragangliomas
Remacha, Comino-Méndez, Richter et al
Clin. Cancer Res. (2017)
Remacha, Comino-Méndez, Richter et al
Clin. Cancer Res. (2017)
AI Summary: OriGene products pCMV6-AC-GOT2 (SC127269) and pCMV6-AC-OGDHL (RC205225) plasmids were used for transient transfection experiments to study the functional impact of GOT2 and OGDHL variants in pheochromocytoma and paraganglioma tumor cells.
Snippet: … Each well was transiently transfected with 2 μg of pCMV6-AC plasmid containing the full cDNA sequence of GOT2 (SC127269, Origene), or OGDHL (RC205225, Origene), or with 2 μg of pCMV6-AC plasmids carrying the corresponding ... …Each well was transiently transfected with 2 μg of pCMV6-AC plasmid containing the full cDNA sequence of GOT2 (SC127269, Origene), or OGDHL (RC205225, Origene), or with 2 μg of pCMV6-AC plasmids carrying the corresponding ... …
Identification of new phaeochromocytoma and paraganglioma susceptibility genes
Remacha Medina
Thesis (2019)
Remacha Medina
Thesis (2019)
Snippet: … UNIVERSIDAD AUTONOMA DE MADRID Departamento de Bioquimica Doctoral Thesis Identification of new phaeochromocytoma and paraganglioma susceptibility genes Laura Remacha Medina Madrid, 2019 Page 2 View as HTML;Each well was transiently transfected with 2 mg of pCMV6-AC plasmid containing the full cDNA sequence of GOT2 (SC127269, Origene), or OGDHL (RC205225, Origene), or with 2 mg of pCMV6-AC plasmids carrying the corresponding variant (GOT2 c.357AT and c.223TG, or OGDHL c.750GT) using Lipofectamine 2000 (Invitrogen), according to the manufacturer's recommendations
Showing 1-2 of 2 papers.
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