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EID1 (NM_014335) Human Untagged Clone

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SKU
SC127263
EID1 (untagged)-Human EP300 interacting inhibitor of differentiation 1 (EID1)
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EID1
Synonyms C15orf3; CRI1; EID-1; IRO45620; PNAS-22; PTD014; RBP21
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC127263 sequence for NM_014335 edited (data generated by NextGen Sequencing)
ATGTCGGAAATGGCTGAGTTGTCCGAGCTGTATGAAGAGAGCAGTGACCTGCAGATGGAT
GTGATGCCTGGCGAGGGTGACCTTCCGCAGATGGAGGTAGGCAGCGGGAGCCGGGAGCTA
TCCCTGCGTCCCTCCCGCAGCGGGGCCCAACAGCTCGAGGAGGAAGGCCCAATGGAGGAG
GAGGAGGCCCAGCCAATGGCGGCGCCAGAGGGGAAACGGAGCCTTGCTAACGGGCCCAAC
GCTGGGGAGCAGCCAGGCCAGGTGGCGGGCGCAGACTTCGAGAGCGAGGACGAGGGCGAG
GAATTTGATGACTGGGAGGACGACTACGACTATCCCGAAGAGGAGCAGCTCAGTGGTGCC
GGCTACAGAGTATCAGCCGCTCTTGAAGAAGCCGACAAGATGTTTCTGAGAACAAGAGAA
CCAGCCCTGGATGGCGGGTTTCAGATGCATTATGAGAAGACCCCGTTTGATCAGTTAGCT
TTTATCGAAGAGCTTTTTTCACTGATGGTTGTCAATCGTCTGACCGAAGAACTCGGCTGT
GATGAGATTATTGATAGAGAGTAG

Clone variation with respect to NM_014335.2
5' Read Nucleotide Sequence
>OriGene 5' read for NM_014335 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGAAGCTTTTT
CCTAGAGGTTGAGCGGTTTGCACAATGTCGGAAATGGCTGAGTTGTCCGAGCTGTATGAA
GAGAGCAGTGACCTGCAGATGGATGTGATGCCTGGCGAGGGTGACCTTCCGCAGATGGAG
GTAGGCAGCGGGAGCCGGGAGCTATCCCTGCGTCCCTCCCGCAGCGGGGCCCAACAGCTC
GAGGAGGAAGGCCCAATGGAGGAGGAGGAGGCCCAGCCAATGGCGGCGCCAGAGGGGAAA
CGGAGCCTTGCTAACGGGCCCAACGCTGGGGAGCAGCCAGGCCAGGTGGCGGGCGCAGAC
TTCGAGAGCGAGGACGAGGGCGAGGAATTTGATGACTGGGAGGACGACTACGACTATCCC
GAAGAGGAGCAGCTCAGTGGTGCCGGCTACAGAGTATCAGCCGCTCTTGAAGAAGCCGAC
AAGATGTTTCTGAGAACAAGAGAACCAGCCCTGGATGGCGGGTTTCAGATGCATTATGAG
AAGACCCCGTTTGATCAGTTAGCTTTTATCGAAGAGCTTTTTTCACTGATGGTTGTCAAT
CGTCTGACCGAAGAACTCGGCTGTGATGAGATTATTGATAGAGAGTAGTTAGATGCTGTT
AAAAGAGGAGGAAACTACTTGAGGAGGGACCCAACTTTCCGCTATCTTTTGGGTTCATTC
CAAATAGTTTTGTGCCATTGAAAAACTTGACCTTCAAAAAATTTGTTTTTCAGAATAGAA
ACACATAGGACAGTGACTGCACAGTTGTGAAAAAGGAGAGAATCCTAAAGAAAAGAAAAA
GATTTTAGAACGNNTGAATCNATATCAGAN
Restriction Sites NotI-NotI |
ACCN NM_014335
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014335.2, NP_055150.1
RefSeq Size 2096 bp
RefSeq ORF 564 bp
Locus ID 23741
UniProt ID Q9Y6B2
Cytogenetics 15q21.1
Summary Interacts with RB1 and EP300 and acts as a repressor of MYOD1 transactivation. Inhibits EP300 and CBP histone acetyltransferase activity. May be involved in coupling cell cycle exit to the transcriptional activation of genes required for cellular differentiation. May act as a candidate coinhibitory factor for NR0B2 that can be directly linked to transcription inhibitory mechanisms.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_014335" in other vectors (4)
SKU Description Size Price
RC223009 EID1 (Myc-DDK-tagged)-Human EP300 interacting inhibitor of differentiation 1 (EID1) 10 ug
$300.00
RC223009L3 Lenti ORF clone of Human EP300 interacting inhibitor of differentiation 1 (EID1), Myc-DDK-tagged 10 ug
$600.00
RC223009L4 Lenti ORF clone of Human EP300 interacting inhibitor of differentiation 1 (EID1), mGFP tagged 10 ug
$600.00
RG223009 EID1 (tGFP-tagged) - Human EP300 interacting inhibitor of differentiation 1 (EID1) 10 ug
$489.00 $500.00

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