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TCHP (NM_032300) Human Untagged Clone

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SKU
SC127206
TCHP (untagged)-Human trichoplein, keratin filament binding (TCHP), transcript variant 1
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TCHP
Synonyms TpMs
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_032300, the custom clone sequence may differ by one or more nucleotides


ATGGCGCTCCCGACGCTGCCGTCCTACTGGTGCAGCCAGCAGCGCCTGAATCAGCAGCTAGCACGACAGC
GAGAGCAGGAGGCCCGGCTTCGGCAGCAGTGGGAGCAGAACAGCCGTTACTTCAGGATGTCTGACATCTG
CAGCTCCAAACAGGCAGAATGGAGCTCTAAAACCTCCTACCAGCGGAGCATGCATGCCTATCAGCGGGAG
AAGATGAAGGAGGAGAAGAGGAGGAGTCTGGAGGCCCGACGGGAAAAGCTCAGGCAGCTCATGCAGGAGG
AGCAGGACCTGCTGGCCAGAGAACTGGAGGAGCTGAGGCTGAGCATGAACTTGCAGGAAAGAAGAATCCG
GGAGCAGCACGGGAAGCTGAAATCAGCCAAAGAAGAGCAGAGGAAACTGATTGCTGAACAACTTTTGTAC
GAACACTGGAAAAAGAACAACCCGAAACTTCGAGAGATGGAGCTGGACCTTCACCAGAAGCATGTCGTAA
ACTCTTGGGAAATGCAGAAAGAAGAAAAAAAACAGCAAGAAGCCACCGCAGAGCAAGAGAACAAACGGTA
TGAAAATGAATATGAAAGGGCCCGAAGGGAGGCGCTAGAAAGGATGAAAGCTGAAGAGGAGAGGAGGCAG
CTGGAGGACAAGCTCCAGGCCGAGGCACTGCTGCAACAGATGGAGGAGCTGAAGCTGAAGGAGGTGGAGG
CGACCAAACTAAAGAAGGAGCAGGAGAATCTGTTGAAGCAGCGGTGGGAGCTAGAGAGGCTGGAGGAAGA
GCGAAAGCAGATGGAAGCCTTCCGGCAGAAGGCAGAGCTGGGGCGTTTCTTGAGACATCAGTATAACGCT
CAACTCAGCAGACGCACACAGCAGATCCAAGAGGAGCTGGAGGCAGACAGGCGGATCCTGCAGGCCCTCC
TCGAGAAGGAGGACGAGAGCCAGCGCCTCCACCTGGCCAGGCGGGAGCAGGTCATGGCCGATGTGGCCTG
GATGAAGCAGGCCATTGAGGAGCAGCTGCAGCTGGAGCGGGCGCGGGAGGCAGAGCTGCAGATGCTGCTG
AGGGAGGAGGCCAAGGAGATGTGGGAAAAGAGAGAGGCAGAGTGGGCCCGAGAGCGCAGCGCACGGGACA
GACTGATGAGCGAGGTTCTGACAGGGAGACAACAGCAAATACAAGAGAAGATTGAGCAGAACCGACGGGC
ACAAGAGGAATCCCTGAAACACAGGGAGCAACTTATTCGAAATCTTGAGGAGGTGAGAGAGTTGGCTCGT
CGCGAGAAAGAGGAGAGTGAAAAGCTGAAATCGGCCAGGAAGCAGGAGCTGGAAGCCCAGGTTGCAGAGC
GCCGGCTGCAGGCATGGGAAGCAGACCAGCAGGAGGAGGAGGAAGAGGAGGAGGCCCGGCGGGTCGAGCA
GCTCTCAGATGCCCTGCTGCAGCAGGAGGCGGAGACTATGGCTGAGCAGGGCTACCGGCCTAAGCCTTAC
GGACATCCAAAAATTGCTTGGAACTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_032300 unedited
GTCAGAAATTTGTAATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGGAAACA
GCCGGCGTTGCTGTAGCGCGTCTGGGAATTACACCGGGGGACTGGCCGGCCCGCTTCGTG
CCTGCGGGAAGTCGGGCCGGGGGACTCTTCGGAAACTCCGAGCCTCAGAGAAATATGGCG
CTCCCGACGCTGCCGTCCTACTGGTGCAGCCAGCAGCGCCTGAATCAGCAGCTAGCACGA
CAGCGAGAGCAGGAGGCCCGGCTTCGGCAGCAGTGGGAGCAGAACAGCCGTTACTTCAGG
ATGTCTGACATCTGCAGCTCCAAACAGGCAGAATGGAGCTCTAAAACCTCCTACCAGCGG
AGCATGCATGCCTATCAGCGGGAGAAGATGAAGGATGAGAAGAGGAGGAGTCTGGAGGCC
CGACGGGGAAAGCTCAGGCAGCTCATGCAGGATGAGCATGACCTGCTGGCCANAGAACTG
GAGGAGCTGAGGCTGAGCATGAACTTGCAAGAAAGAAGAATCCCGGAGCAGCACGGGAAG
CTGAAATCAGCCAGAGAGAGCAGAGGAAACTGATTGGGCTGACACTTTTGACGACACTGG
AAAAAGAACACCCCCGAACTCGAGAGATGGAGCTGGACCTCACCAGAGCATGTCGTAACT
CTGGGGAAATGCNGAAGATGATAAAAACAGCAGAGCCCCCGCAAGCAGAGACCAACGTAT
GATATGATATGNAAGCCCCGANGGGAGCGCTAAAGATGAACCCCAAAAAGAAAAGAGGCA
GCTGGAGACAGCTCCAGGCGAGGCCTGCTGCACAGATGGAGAGCTGAGCTGAAAGAGGTG
GAGGCGACCCAACTAAGATGAGCGGAGAATCTGTGAANCACGGTGGAGCTNAGAGGCTGG
AGAAACGAAGCNATGGATCT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_032300 unedited
CAGCTTGGCCCGAAGCCGCAATCNANGATCGAGTTTTTTTTCTTTTTTTTTTTTTTTTTT
TTGGCGAAGCAGATTTTATTTCACCATTACTGGACATCTGAAATGGGGAACGCAACAGAG
AGCATGCGTGATGAACGTGCTGCTCAGGGGATCAGAGTCAAAGAGTTGCCAGAAAATGAA
CAACAGATGAAGGACGTGGTTTGCAGCTTGATCTGTTTTTACAGGACAGTGGAAGTGAAA
GGTAAGACAGCCCTTAAGACTCAGGATTTTTAATAAACTCTGATTTTTAATAACCATGGA
ACAATGCCCTGTAATTAGCTGTAATTTACGGAGATATGGAATCTTGAACATAATGGGATG
AAAACTAAACCTCAGAGGAAGGGTATAGTAAAAATACCAGTCATAAAATAATATTATTGC
TGCAAGGAACTTGTTCCTGTTTGTAGAAATGAATGTACACCTGTTTCACGCTGATATAAT
GGGGGGAGAGATTTCCGGCCGGTCTGTGGTTCTGAGACTCATCTAGTTTCCTTCGGCTCA
GAGCTTTTCCCTCTGGGCCCATATATCCCACGAAGCTACATGTTGTTCCTCAGACATTCC
CATGTGGCCTACGTGTTCCCCCCCTGCCTTTTAAAGGGTTCCTCCCCCGGTCGACCCTCT
CAGGGCCCTTCTTGGTCTTAAAGTAGGGCCTCACTCTTTTGCTCCCTTGGCCTCCACCCC
CGGGCTCAACAGATTCTTTTTCCTTAGCCCTTCCGGGTAGCGGGCCCTAAGGACCCTTCC
CCCCCCCCGCATTCGAAACTATTCTTCGGGCCTCCTAACCCTCTGTTGGGATCCTTCTCT
CCCACCCTTGGCTTTTCGAAATTTTCCCCAATGGCTCCTGGAGGAGGGAGACTCTTTGTT
CCTCTCTTTTGTAAGCACCCATGACCCCCACTCACCGCGC
Restriction Sites NotI-NotI |
ACCN NM_032300
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032300.2, NP_115676.1
RefSeq Size 3162 bp
RefSeq ORF 1497 bp
Locus ID 84260
UniProt ID Q9BT92
Cytogenetics 12q24.11
Summary Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells (PubMed:15731013, PubMed:18931701). Is a negative regulator of ciliogenesis (PubMed:25270598).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.
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"NM_032300" in other vectors (4)
SKU Description Size Price
RC202566 TCHP (Myc-DDK-tagged)-Human trichoplein, keratin filament binding (TCHP), transcript variant 1 10 ug
$457.00
RC202566L3 Lenti ORF clone of Human trichoplein, keratin filament binding (TCHP), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC202566L4 Lenti ORF clone of Human trichoplein, keratin filament binding (TCHP), transcript variant 1, mGFP tagged 10 ug
$757.00
RG202566 TCHP (tGFP-tagged) - Human trichoplein, keratin filament binding (TCHP), transcript variant 1 10 ug
$657.00

file-alt Citations

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