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AGAP1 (NM_014914) Human Untagged Clone

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SKU
SC127122
AGAP1 (untagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (AGAP1), transcript variant 2
  $737.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol AGAP1
Synonyms AGAP-1; CENTG2; cnt-g2; GGAP1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_014914 edited
ATGAACTACCAGCAGCAGCTGGCCAACTCGGCTGCCATCCGGGCCGAGATCCAGCGCTTC
GAGTCGGTCCACCCCAACATCTACTCCATCTACGAGCTGCTGGAGCGCGTGGAGGAGCCG
GTGCTGCAGAACCAGATCCGGGAGCACGTCATCGCCATCGAAGATGCCTTCGTGAACAGC
CAGGAATGGACGCTGAGTCGATCTGTCCCGGAGCTCAAAGTGGGAATTGTGGGTAACTTG
GCCAGCGGCAAGTCTGCCCTGGTGCACCGGTACCTGACGGGCACATATGTCCAGGAGGAG
TCTCCGGAAGGTGGCAGGTTCAAGAAAGAGATTGTCGTTGATGGACAGAGCTATCTGCTG
CTGATCAGAGATGAAGGGGGCCCCCCGGAGGCGCAGTTTGCCATGTGGGTGGACGCTGTT
ATATTTGTCTTCAGCTTGGAGGATGAAATAAGTTTCCAGACCGTTTACCACTACTACAGT
CGAATGGCCAACTATCGGAACACGAGCGAGATTCCTCTGGTTCTGGTGGGAACCCAGGAT
GCCATAAGTTCTGCTAACCCGAGGGTCATCGATGACGCCAGGGCGAGGAAGCTCTCCAAC
GACCTGAAACGGTGCACGTACTACGAGACGTGTGCTACATACGGGCTGAATGTGGAGAGG
GTCTTCCAGGACGTTGCCCAGAAGATTGTTGCCACAAGGAAGAAGCAGCAGCTGTCCATA
GGACCCTGCAAGTCGCTACCTAATTCTCCCAGCCATTCCTCCGTCTGTTCCGCGCAGGTG
TCTGCCGTGCACATCAGCCAGACAAGTAATGGAGGTGGGAGTTTAAGCGACTATTCCTCC
TCCGTTCCATCGACTCCCAGCACCAGCCAGAAGGAACTTCGGATCGATGTTCCTCCCACT
GCCAACACGCCCACGCCCGTTCGCAAGCAGTCTAAGCGCCGGTCCAACCTGTTCACCTCT
CGGAAAGGGAGCGACCCAGACAAAGAGAAGAAAGGCCTGGAGAGTCGTGCGGACAGCATT
GGGAGCGGCCGAGCCATCCCAATTAAACAGGGCATGCTGTTGAAGCGAAGTGGCAAATCG
TTGAATAAAGAGTGGAAAAAGAAATATGTCACCCTGTGTGACAATGGCGTGCTGACCTAT
CATCCCAGTTTACATGATTACATGCAGAATGTTCATGGTAAGGAGATTGACCTTCTGAGA
ACCACTGTGAAAGTCCCAGGGAAGAGGCCACCCCGAGCCACGTCAGCCTGCGCACCCATC
TCCAGCCCTAAAACCAATGGCCTATCCAAGGACATGAGCAGTTTACACATCTCACCCAAT
TCAGGGAATGTCACTAGTGCATCTGGGTCTCAGATGGCAAGCGGCATCAGCCTGGTCTCC
TTCAACAGCCGACCCGACGGCATGCACCAGCGCTCCTACTCAGTCTCCAGTGCCGA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_014914 unedited
GATTTGTAAACGACTTACTATAGGCGGCCGCGACATTCGCACCAGCTCCTGGGGGCGCGC
TCCCCCCGGGCGGCCCGCTGACCCGGGACGCCGGGGCCCGCTCGCTCGCCGGCCGCGCGT
CCCGGCCATGAACTGAGCCCGCGGGCCAGCCCCGCGCCTGCTCCGCCCGCGCCTTTCTTC
TCGCGCCTCCTCCGCCCGCCGCCGGCGGGCCCGGCTCCCCGGGGGCTGCGGCGCCCCGGG
CTCGGCGGCCCGCGGGCCCCGGGGCGCGGGGCGGCGGCGGCGGGGGGCGCGCGGCTCCGG
GCGCGGCGCCTGCACCATGAACTACCAGCAGCAGCTGGCCAACTCGGCTGCCATCCGGGC
CGAGATCCAGCGCTTCGAGTCGGTCCACCCCAACATCTACTCCATCTACGAGCTGCTGGA
TCGCGTGGAGGAGCCGGTGCTGCAGAACCAGATCCGGGAGCACGTCATCGCCATCGAAGA
TGCCTTCGTGAACAGCCAGGAATGGACGCTGAGTCGATCTGTCCCGGAGCTCAAAGTGGG
AATTGCGGGTAACTAGGCCAGCGGCAAGTCTGCCCTGGGGCACCGGTCCCTGACAGGCAC
ATATGTCCACGAGGAGTCTCCGGAAAGAGGCAGTTCCAAGAAGAGATAGTCGCTGATGGA
CAGAGCTATCTGCTGCTGATCATAGATGAAAGGGGCCCCCCCGAAGCGCAGTTTGCCCTG
TGGGGTGGACGCTGGTCTATTCGTCCTCAGCCTGGAAGATGAACTAATTCCCAGAACGGC
TACACCTCTACAGTCCAATGGCCAACTTCCGAAACCGAACGCAATACCTCCGGCCTTGGG
GGGAACCCACAAGCCTCACTTCTGCTAACCGAGGGTCTTCTTGANCCCAGGGCAAGGACC
TCTCCACCACCGGAACGGCCCGCACTCCAACACGGGGTCCACCGCCCTCAAGGGCAAAGT
TCTTCCGCCTCCGCCCAAAAAGGTGCCCACAGAAAAAAT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_014914 unedited
NGGAAGATACATTATTNTACCGCGGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTT
GCATATTTATGAACTTCTTTTATTCAAATATATTTTCACACATCTTATCTAAATACATAA
CACAGAAGCCTGTGTGACTTGGGCAACGTGGTCCAGGAGGGCCTGAGACTAACACATCCA
CCTCGGCAAAAGGACATCAAATATCTCTTACAGTGCGGAAAAAACAGCCTTTTGTGTATT
TCCTTAGTTTACGAAATATACTCGAAATGCTATTATTAGCTGAATTTGTGGTTTCCTTTT
GAGTTTCTGAGTTATTCTTATTTATTTTTCCCATTTTGTTTTTGCACCAAGGAGACCGGA
GTCAAATAATACTCAGCGACTGATTTCCTCTCTTTGGACTGAAAAATTAAACAGATACTA
AATGATGACAGTGAATTTAGAGAGGGCTCCAAGGGCTTGAAAGAACATGTCTGGGATAAT
ATGGTGCTTCTAAGAGTATTGCAATCACATCGTGGCAATCACCGGCGCGTGCCGCGTGAC
TACCTCCTCGGCTAATATGCTTTCTTCTCGGCGATGACTAATCACGTTCTATTAAACAGC
AGTAATGCGGGAAGAACTCGGCTGTACAAGTGTAATGAAGCCAGTATTCTCCCTGGACAG
ATTAGCTACAACTGATTTGACACACACCATCATAGGGGCTTTAAACCTGACAAACTCTGG
GCTTGCTTCTGATTTATGCCCGTCGAGCTCCTCAGAGAAAGAGAGGAATCCAAGTGTTCC
CTTTGGTAGAGGGAAAAGTACTATGTGCCTCACTGCCCGTTAAGGAAGTTGCCCCTTCTT
CTTGTTCCCGC
Restriction Sites NotI-NotI |
ACCN NM_014914
Insert Size 5000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014914.2, NP_055729.1
RefSeq Size 4078 bp
RefSeq ORF 2415 bp
Locus ID 116987
UniProt ID Q9UPQ3
Cytogenetics 2q37.2
Domains ANK, ArfGap, PH, RAB, RAS
Protein Pathways Endocytosis
Summary This gene encodes a member of an ADP-ribosylation factor GTPase-activating protein family involved in membrane trafficking and cytoskeleton dynamics. This gene functions as a direct regulator of the adaptor-related protein complex 3 on endosomes. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2011
Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
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"NM_014914" in other vectors (4)
SKU Description Size Price
RC214836 AGAP1 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (AGAP1), transcript variant 2 10 ug
$736.00
RC214836L3 Lenti-ORF clone of AGAP1 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (AGAP1), transcript variant 2 10 ug
$1,036.00
RC214836L4 Lenti-ORF clone of AGAP1 (mGFP-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (AGAP1), transcript variant 2 10 ug
$1,036.00
RG214836 AGAP1 (tGFP-tagged) - Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (AGAP1), transcript variant 2 10 ug
$489.00 $936.00

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