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DHPS (NM_013407) Human Untagged Clone

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SKU
SC126747
DHPS (untagged)-Human deoxyhypusine synthase (DHPS), transcript variant 3
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DHPS
Synonyms DHS; MIG13
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>NCBI ORF sequence for NM_013407, the custom clone sequence may differ by one or more nucleotides


ATGGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTGCTAAAGCACAGCTCGACGT
TGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAACCGCGGTGTGAATTACCGCGCACTGCTGGA
GGCCTTCGGCACCACCGGCTTCCAAGCAACCAACTTCGGGCGCGCTGTACAGCAAGTCAATGCCATGATC
GAGAAGAAGCTGGAACCACTGTCACAGGATGAAGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCAC
TTACCAGCTGCACCATTTTCCTGGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCG
CTACCTTGTGCAGCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAAGACCTCATC
AAGTGCCTGGCGCCCACATACTTGGGCGAGTTTAGCCTCAGGGGGAAGGAGCTCCGGGAGAACGGGATCA
ATAGGATCGGAAACCTGCTGGTGCCCAATGAGAATTACTGCAAGTTTGAGGACTGGCTGATGCCCATTCT
GGACCAGATGGTGATGGAGCAGAACACAGAGGGTGTAAAGTGGACGCCTTCTAAGATGATCGCCCGGCTG
GGCAAGGAGATCAACAACCCAGAGTCCGTGTATTACTGGGCCCAGAAGAACCACATCCCTGTGTTTAGTC
CCGCACTTACAGACGGCTCGCTGGGCGACATGATCTTCTTCCATTCCTACAAGAACCCGGGCCTGGTCCT
GGACATCGTTGAGGGTGCCCGACCAGACGAGGCTGTCTCCTGGGGCAAGATCCGGGTGGATGCACAGCCC
GTCAAGGTCTATGCTGACGCCTCCCTGGTCTTCCCCCTGCTTGTGGCTGAAACCTTTGCCCAGAAGATGG
ATGCCTTCATGCATGAGAAGAACGAGGACTGA

5' Read Nucleotide Sequence
>OriGene 5' read for NM_013407 unedited
GGCCGCGAAATTCGCACGAGGCTCTTGCGGAGACGCGCGCGTCGGGGTTTAACGCGTTTC
TGGGCCGCCGTAAGCCCGGCCTAGGGGCAGCTTTGACTCGAGAGCCGGCTATAGGCGCAT
GGAAGGTTCCCTGGAACGGGAGGCGCCAGCGGGGGCGCTGGCCGCCGTGCTAAAGCACAG
CTCGACGTTGCCGCCCGAAAGCACCCAGGTCCGGGGCTACGACTTCAACCGCGGTGTGAA
TTACCGCGCACTGCTGGAGGCCTTCGGCACCACCGGCTTCCAAGCAACCAACTTCGGGCG
CGCTGTACAGCAAGTCAATGCCATGATCGAGAAGAAGCTGGAACCACTGTCACAGGATGA
AGACCAGCACGCGGACCTGACCCAGAGCCGCCGCCCACTTACCAGCTGCACCATTTTCCT
GGGATATACATCCAACCTCATCAGTTCAGGCATCCGTGAGACCATTCGCTACCTTGTGCA
GCACAACATGGTGGACGTATTGGTGACCACAGCTGGCGGCGTGGAGGAAGACCTCATCAA
GTGCCTGGCGCCCACATACTTGNGGCGAGTTTAGCCTCAGGGGGGAAGGAGCTCCGGGAG
AACGGGATCAATAGGATCGGGAACCTGGCTGGTGCCCAATGAGAATTACTGGCAGTTTTG
AGGACTGGCTGATGCCCATTCTGGACCAGATGGTGATGGAGCAGACACAGNAGGTGTTAA
AGTGGACGCCTCTTAGATGATCGCCCCGCTGGCCAAGAGATCACACCCAGAGTCCGGGTA
TTACTGGGCCCAGAGACCACTTCCTGGGTTTAGTCCGCCTTACGACGGNTTGTTGGCAAT
TGATCTTTTCCATCA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_013407 unedited
ACCGGCGGCCGCAATCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTGGGACTTCAAATACCATCTTATTTTAAAAACCTAACTGACCAAAAAATAAGGGAGGG
GCTGGGTCTGCAAATTAATAAATAAAAAAGGGGGGAAAAACTTTCTGGGACCCCAACCGC
TCAATCCTCGTTCTTTTCATGCCTGAAGGCATCCATTTTTTGGGCAAAGGTTTCAACCAC
AAGCAGGGGGAAAACCAAGGAGGCGTCAACATAAACCTTGACGGGCTGTGCATCCACCCG
GATCTTGCCCCAAGAAACAACCTCGTTTGGTCGGGCACCTGAGTCAAAACCCTCAAACTC
CTGGGCTGGGTTGATGTAAACAACGTAATCGGCCCCCTTCCCCATGAGGGTGGCATTGGC
AATGTGGGGCTTGACCACCCCCCCGCCCAAAATGATCATCCCAATGCACTTGGCAAAAAT
GGCCTGTGTGTTGATGAACCTCAAGGCCTCAACGATGTCCAAGACCAAGCCCGGGTTCTT
GTAGGAATGGAAAAAGATCATGTCGCCCAACGAGCCGTCTGTAAGTGCGGGACTAAACAC
AGGGATGTGGGTCTTCTGGGCCCAATAATACACGGACTCTGGGTTGGTGATCTCCTTGCC
CAACCGGGCGATCATCTTAAAAGGCGTCCACTTTACACCCTCTGTGGTCTGGTCCATCAC
CATACTGGCCAAAATGGGGATTAACCCGTTCTCAAACTTGCAGTAATCTTATTGGGCACC
ACCAAGTTTCCAACCTATGATCCCCGTCTCCCCGGACTCCTTTCCCTGAAGCTAACTCGC
CCAATTTGGGGCGCCCAGCACTGATGAAGCTTTCCTCCCCCCCCACTGTGGGACCAATAC
CTCCACATTTGGGCTCCAAAGGACCAAAGGGCTCCCGGATCCCTAACTGTGGAGGTGGAA
GTATATTCCAAAAATGTGCACCTTGTAATGGCCGCGGCTTGGGCTAGCCCCCA
Restriction Sites NotI-NotI |
ACCN NM_013407
Insert Size 1260 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013407.1, NP_037539.1
RefSeq Size 1183 bp
RefSeq ORF 942 bp
Locus ID 1725
Cytogenetics 19p13.13
Domains DS
Summary This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, May 2011
Transcript Variant: This splice variant (3) lacks exon 7 and includes alternative exon 8a.
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Documents
Resources
"NM_013407" in other vectors (4)
SKU Description Size Price
RC222934 DHPS (Myc-DDK-tagged)-Human deoxyhypusine synthase (DHPS), transcript variant 3 10 ug
$300.00
RC222934L3 Lenti ORF clone of Human deoxyhypusine synthase (DHPS), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC222934L4 Lenti ORF clone of Human deoxyhypusine synthase (DHPS), transcript variant 3, mGFP tagged 10 ug
$600.00
RG222934 DHPS (tGFP-tagged) - Human deoxyhypusine synthase (DHPS), transcript variant 3 10 ug
$489.00 $500.00

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