cIAP1 (BIRC2) (NM_001166) Human Untagged Clone

SKU SC126567

BIRC2 (untagged)-Human baculoviral IAP repeat containing 2 (BIRC2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: cIAP1
• Synonyms: API1; c-IAP1; cIAP1; Hiap-2; HIAP2; MIHB; RNF48
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126567 sequence for NM_001166 edited (data generated by NextGen Sequencing); ATGCACAAAACTGCCTCCCAAAGACTTTTCCCAGGTCCCTCGTATCAAAACATTAAGAGT; ATAATGGAAGATAGCACGATCTTGTCAGATTGGACAAACAGCAACAAACAAAAAATGAAG; TATGACTTTTCCTGTGAACTCTACAGAATGTCTACATATTCAACTTTCCCCGCCGGGGTG; CCTGTCTCAGAAAGGAGTCTTGCTCGTGCTGGTTTTTATTATACTGGTGTGAATGACAAG; GTCAAATGCTTCTGTTGTGGCCTGATGCTGGATAACTGGAAACTAGGAGACAGTCCTATT; CAAAAGCATAAACAGCTATATCCTAGCTGTAGCTTTATTCAGAATCTGGTTTCAGCTAGT; CTGGGATCCACCTCTAAGAATACGTCTCCAATGAGAAACAGTTTTGCACATTCATTATCT; CCCACCTTGGAACATAGTAGCTTGTTCAGTGGTTCTTACTCCAGCCTTTCTCCAAACCCT; CTTAATTCTAGAGCAGTTGAAGACATCTCTTCATCGAGGACTAACCCCTACAGTTATGCA; ATGAGTACTGAAGAAGCCAGATTTCTTACCTACCATATGTGGCCATTAACTTTTTTGTCA; CCATCAGAATTGGCAAGAGCTGGTTTTTATTATATAGGACCTGGAGATAGGGTAGCCTGC; TTTGCCTGTGGTGGGAAGCTCAGTAACTGGGAACCAAAGGATGATGCTATGTCAGAACAC; CGGAGGCATTTTCCCAACTGTCCATTTTTGGAAAATTCTCTAGAAACTCTGAGGTTTAGC; ATTTCAAATCTGAGCATGCAGACACATGCAGCTCGAATGAGAACATTTATGTACTGGCCA; TCTAGTGTTCCAGTTCAGCCTGAGCAGCTTGCAAGTGCTGGTTTTTATTATGTGGGTCGC; AATGATGATGTCAAATGCTTTTGTTGTGATGGTGGCTTGAGGTGTTGGGAATCTGGAGAT; GATCCATGGGTAGAACATGCCAAGTGGTTTCCAAGGTGTGAGTTCTTGATACGAATGAAA; GGCCAAGAGTTTGTTGATGAGATTCAAGGTAGATATCCTCATCTTCTTGAACAGCTGTTG; TCAACTTCAGATACCACTGGAGAAGAAAATGCTGACCCACCAATTATTCATTTTGGACCT; GGAGAAAGTTCTTCAGAAGATGCTGTCATGATGAATACACCTGTGGTTAAATCTGCCTTG; GAAATGGGCTTTAATAGAGACCTGGTGAAACAAACAGTTCAAAGTAAAATCCTGACAACT; GGAGAGAACTATAAAACAGTTAATGATATTGTGTCAGCACTTCTTAATGCTGAAGATGAA; AAAAGAGAAGAGGAGAAGGAAAAACAAGCTGAAGAAATGGCATCAGATGATTTGTCATTA; ATTCGGAAGAACAGAATGGCTCTCTTTCAACAATTGACATGTGTGCTTCCTATCCTGGAT; AATCTTTTAAAGGCCAATGTAATTAATAAACAGGAACATGATATTATTAAACAAAAAACA; CAGATACCTTTACAAGCGAGAGAACTGATTGATACCATTTTGGTTAAAGGAAATGCTGCG; GCCAACATCTTCAAAAACTGTCTAAAAGAAATTGACTCTACATTGTATAAGAACTTATTT; GTGGATAAGAATATGAAGTATATTCCAACAGAAGATGTTTCAGGTCTGTCACTGGAAGAA; CAATTGAGGAGGTTGCAAGAAGAACGAACTTGTAAAGTGTGTATGGACAAAGAAGTTTCT; GTTGTATTTATTCCTTGTGGTCATCTGGTAGTATGCCAGGAATGTGCCCCTTCTCTAAGA; AAATGCCCTATTTGCAGGGGTATAATCAAGGGTACTGTTCGTACATTTCTCTCTTAA; ; Clone variation with respect to NM_001166.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001166 unedited; CTAGGCAGCCGCGCAGCTTCCGTGTTTGCTGCGCCCGCACTGCGATTTACAACCCTGAAG; AATCTCCCTATCCCTATTTTGTCCCCCTGCAGTAATAAATCCCATTATGGAGATCTCGAA; ACTTTATAAAGGGATATAGTTTGAATTCTATGGAGTGTAATTTTGTGTATGAATTATATT; TTTAAAACATTGAAGAGTTTTCAGAAAGAAGGCTAGTAGAGTTGATTACTGATACTTTAT; GCTAAGCAGTACTTTTTTGGTAGTACAATATTTTGTTAGGCGTTTCTGATAACACTAGAA; AGGACAAGTTTTATCTTGTGATAAATTGATTAATGTTTACAACATGACTGATAATTATAG; CTGAATAGTCCTTAAATGATGAACAGGTTATTTAATTNTTAAATGCAGTGTAAAAAGTGT; GCTGTGGAAATTTTATGGCTAACTAAGTTTATGGAGAAAATACCTTCAGTTGATCAAGAA; TAATAGTGGTATACAAAGTTAGGAAGAAAGTCACATGATGCTGCANGAAATGGGAACANA; TACCAATGATATTTAACAAAGATAGAGTTTACAGTTTTTGAACTTTAAGCCAAATTCATT; TGACATCAAGCACTATAGCAGGCACAGGTTCAACAAAGCTTGTGGGGTATTGACTTCCCC; CCAAAAGTTGTTCACCTGAAGTAATTTAGCCCCCCTTTAGTAAATACCTATGAAGATATA; GCTGGGGTGAACCTAAGCTTNTAAATAAGTGTGACCATATAGAAGGTTTTAATTACTTTT; NGTTATTNGGAATAAATAGAGACTCTTGGGGTTGGCTTGTTAAAGGCCTTATAGGGAAAG; AACCTGCATTTAATTTTCACCTTGGGCATAACAGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001166 unedited; ATTTTAATACCACATGTGAAAAGCACCAGAGACAATTCGGCACCATAACTCTGATGAACT; CCAGAGTTCCTGGTTTCCAGTTCTTTTCTACACTATAATTTAGTATTCAAAGCTTTCTGA; AAGAACAACAAATCCAGTAACTCCTGATTTGATGCATAACAAGCAGTGACACTACTCCCT; TAGGTACGCATACATACTAATATAGTTCACCAAACATAAATCTTCCCTTGTAAATAAACA; GATTAAGATTAAATATATGATACCATCTTTTCAGAAACAAGATTATTAGTACCAAAGCAG; ACTAGAACATGAATGTTACTAATTGAAAAACTCATAATTCCCTTTTTACTTTAGATGGCT; TCAAGTGTTCAACAATATTTTAAAGACCTTTTCATGCACGTTAAAATATAGACTATTTTT; CTTTAAGAGAGAAATGTACGAACAGTACCAGTGATTATACGCCTGCAAATACGGCATTTT; CTTAGAGAAGGGGCACATTCCCTGGCATACTACCCGATGACCACAGGGAATAAATACAAC; AGAGACTTCTTTGTCCATGCCCGCTTTACAAGTTGGTTCTGGTTGCCGCCCGGTGAGAGG; GGCTTCGGGGGACCGGACCGGAAGACACGTGCGGATCGCAAGATACCTTGATCACGGTTT; GCGCGGGCCCAATTTCTGATGCATTCGGCGAGTGCATGTCGTTATCCACACGCATGCGGA; GGACCTGCGCGGGCGCCGTCCGCGTGGCGGCCACTTGCGGGCTCTCCAGGGTCTGGGCGG; ATGTAGCGCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001166
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001166.3, NP_001157.1
• RefSeq Size: 3753 bp
• RefSeq ORF: 1857 bp
• Locus ID: 329
• UniProt ID: Q13490
• Cytogenetics: 11q22.2
• Domains: BIR, CARD, RING
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Focal adhesion, NOD-like receptor signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
• Summary: The protein encoded by this gene is a member of a family of proteins that inhibits apoptosis by binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2, probably by interfering with activation of ICE-like proteases. This encoded protein inhibits apoptosis induced by serum deprivation and menadione, a potent inducer of free radicals. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]; Transcript Variant: This variant (1) is the predominant transcript. Variants 1 and 2 encode the same isoform (1).