LONP2 (NM_031490) Human Untagged Clone

SKU SC126500

LONP2 (untagged)-Human lon peptidase 2, peroxisomal (LONP2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: LONP2
• Synonyms: LONP; LONPL; PLON; PSLON
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126500 sequence for NM_031490 edited (data generated by NextGen Sequencing); ATGTCATCAGTGAGCCCCATCCAGATCCCCAGTCGCCTCCCGCTGCTGCTCACCCACGAG; GGCGTCCTGCTGCCCGGCTCCACCATGCGCACCAGCGTGGACTCGGCCCGCAACCTGCAG; CTGGTGCGGAGCCGCCTTCTGAAGGGCACGTCGCTGCAAAGCACCATCCTGGGCGTCATC; CCCAACACGCCTGACCCCGCCAGCGACGCGCAGGACCTGCCGCCGCTGCACAGGATTGGC; ACAGCTGCACTGGCCGTTCAGGTTGTGGGCAGTAACTGGCCCAAGCCCCACTACACTCTG; TTGATTACAGGCCTATGCCGTTTCCAGATTGTACAGGTCTTAAAAGAGAAGCCATATCCC; ATTGCTGAAGTGGAGCAGTTGGACCGACTTGAGGAGTTTCCCAACACCTGTAAAATGAGG; GAGGAGCTAGGAGAACTATCAGAGCAGTTTTACAAATATGCAGTACAATTGGTTGAAATG; TTGGATATGTCTGTCCCTGCAGTTGCTAAATTGAGACGTCTTTTAGATAGTCTTCCAAGG; GAAGCTTTACCAGACATCTTGACATCAATTATCCGAACAAGCAACAAAGAGAAACTCCAG; ATTTTAGATGCTGTGAGCCTAGAGGAGCGGTTCAAGATGACTATACCACTGCTTGTCAGA; CAAATTGAAGGCCTGAAATTGCTTCAAAAAACCAGAAAACCCAAGCAAGATGATGATAAG; AGGGTTATAGCAATACGCCCTATTAGGAGAATTACACATATCTCAGGTACTTTAGAAGAT; GAAGATGAAGATGAAGATAATGATGACATTGTCATGCTAGAGAAAAAAATACGAACATCT; AGTATGCCAGAGCAGGCCCATAAAGTCTGTGTCAAAGAGATAAAGAGACTCAAAAAAATG; CCTCAGTCAATGCCAGAATATGCTCTGACTAGAAATTATTTGGAACTTATGGTAGAACTT; CCTTGGAACAAAAGTACAACTGACCGCCTGGACATTAGGGCAGCCCGGATTCTTCTGGAT; AATGACCATTACGCCATGGAAAAATTGAAGAAAAGAGTACTGGAATACTTGGCTGTCAGA; CAGCTCAAAAATAACCTGAAGGGCCCAATCCTATGCTTTGTTGGCCCTCCTGGAGTTGGT; AAAACAAGTGTGGGAAGATCAGTGGCCAAGACTCTAGGTCGAGAGTTCCACAGGATTGCA; CTTGGAGGAGTATGTGATCAGTCTGACATTCGAGGACACAGGCGCACCTATGTTGGCAGC; ATGCCTGGTCGCATCATCAACGGCTTGAAGACTGTGGGAGTGAACAACCCAGTGTTCCTA; TTAGATGAGGTTGACAAACTGGGAAAAAGTCTACAGGGTGATCCAGCAGCAGCTCTGCTT; GAGGTGTTGGATCCTGAACAAAACCATAACTTCACAGATCATTATCTAAATGTGGCCTTT; GACCTTTCTCAAGTTCTTTTTATAGCTACTGCCAACACCACTGCTACCATTCCAGCTGCC; TTGTTGGACAGAATGGAGATCATTCAGGTTCCAGGTTATACACAGGAGGAGAAGATAGAG; ATTGCCCATAGGCACTTGATCCCCAAGCAGCTGGAACAACATGGGCTGACTCCACAGCAG; ATTCAGATACCCCAGGTCACCACTCTTGACATCATCACCAGGTATACCAGAGAGGCAGGG; GTTCGTTCTCTGGATAGAAAACTTGGGGCCATTTGCCGAGCTGTGGCCGTGAAGGTGGCA; GAAGGACAGCATAAGGAAGCCAAGTTGGACCGTTCTGATGTGACTGAGAGAGAAGGTTGC; AGAGAACACATCTTAGAAGATGAAAAACCTGAATCTATCAGTGACACTACTGACTTGGCT; CTACCACCTGAAATGCCGATTTTGATTGATTTCCATGCTCTGAAAGACATCCTTGGGCCC; CCGATGTATGAAATGGAGGTATCTCAGCGTTTGAGTCAGCCAGGAGTAGCAATAGGTTTG; GCTTGGACTCCCTTAGGTGGAGAAATCATGTTCGTGGAGGCGAGTCGAATGGATGGCGAG; GGCCAGTTAACTCTGACCGGCCAGCTCGGGGACGTGATGAAGGAGTCCGCCCACCTCGCT; ATCAGCTGGCTCCGCAGCAACGCAAAGAAGTACCAGCTGACCAATGCTTTTGGAAGTTTT; GATCTTCTTGACAACACAGACATCCATCTGCACTTCCCAGCTGGAGCTGTCACAAAAGAT; GGACCATCTGCTGGAGTTACCATAGTAACCTGTCTCGCCTCACTTTTTAGTGGGCGGCTG; GTACGTTCAGATGTAGCCATGACTGGAGAAATTACACTGAGAGGTCTTGTTCTTCCAGTG; GGTGGAATTAAAGACAAAGTGCTGGCGGCACACAGAGCGGGACTGAAGCAAGTCATTATT; CCTCGGAGAAATGAAAAAGACCTTGAGGGAATCCCAGGCAACGTACGACAGGATTTAAGT; TTTGTCACAGCAAGCTGCCTGGATGAGGTTCTTAATGCAGCTTTTGATGGTGGCTTTACT; GTCAAGACCAGACCTGGTCTGTTAAATAGCAAACTGTAG; ; Clone variation with respect to NM_031490.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_031490 unedited; TTGAATCCGATTTCTATAGGCGGCCGCGCAATTCGCACGAGGGGGGTCTGAGGTTTGGTG; ACTGCGGGGCAGGCCGGGGGCAGCTGTCTGTCTGGCTCTTTTTGACAGCCCCCAGTGCGA; AAGGCTGCCAGCATGTCATCAGTGAGCCCCATCCAGATCCCCAGTCGCCTCCCGCTGCTG; CTCACCCACGAGGGCGTCCTGCTGCCCGGCTCCACCATGCGCACCAGCGTGGACTCGGCC; CGCAACCTGCAGCTGGTGCGGAGCCGCCTTCTGAAGGGCACGTCGCTGCAAAGCACCATC; CTGGGCGTCATCCCCAACACGCCTGACCCCGCCAGCGACGCGCAGGACCTGCCGCCGCTG; CACAGGATTGGCACAGCTGCACTGGCCGTTCAGGTTGTGGGCAGTAACTGGCCCAAGCCC; CACTACACTCTGTTGATTACAGGCCTATGCCGTTTCCAGATTGTACAGGTCTTAAAAGAG; AAGCCATATCCCATTGCTGAAGTGGAGCAGTTGGACCGACTTGAGGAGTTTCCCAACACC; TGTAAAATGAGGGAGGAGCTAGGAGAACTATCAGAGCAGTTTTACAAATATGCAGTACAA; TTGGTTGAAATGTTGGATATGTCTGTCCCTGCAGTTGCTAAATTGAGACGTCTTTTAGAT; AGTCTTCCAAGGGAAGCTTTACCAGACATCTTGACATCAATTATCCGAACAAGCACANAG; AGAAACTCCAGATTNTAGATGCTGTGAGCCTAGAGGAGCGGTTCAAGATGACTATACCAC; TGCTTGTCAGACANNATGAAGGCCCTGAATTGCTTCAAAAACCAGAAACCCCAAGCAGAG; ATGATAAGAAGGTTATAGCCATACGCCCTATTTAGAGAATACACTATCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_031490
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_031490.2, NP_113678.2
• RefSeq Size: 4342 bp
• RefSeq ORF: 2559 bp
• Locus ID: 83752
• UniProt ID: Q86WA8
• Cytogenetics: 16q12.1
• Domains: AAA, Lon_C
• Protein Families: Druggable Genome, Protease
• Summary: In human, peroxisomes function primarily to catalyze fatty acid beta-oxidation and, as a by-product, produce hydrogen peroxide and superoxide. The protein encoded by this gene is an ATP-dependent protease that likely plays a role in maintaining overall peroxisome homeostasis as well as proteolytically degrading peroxisomal proteins damaged by oxidation. The protein has an N-terminal Lon N substrate recognition domain, an ATPase domain, a proteolytic domain, and, in some isoforms, a C-terminal peroxisome targeting sequence. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jan 2017]; Transcript Variant: This variant (1) represents the longest transcript and encodes isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.