SCUBE3 (NM_152753) Human Untagged Clone

SKU SC126481

SCUBE3 (untagged)-Human signal peptide, CUB domain, EGF-like 3 (SCUBE3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SCUBE3
• Synonyms: CEGF3; SSFSC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_152753 edited; CTCTCTCCCGGCGAAGCTGGGAATTGGGTGGGATTACACGGAGCAGCCCCGCCGCCGCCG; CTGGCAGAGGCCGGCTTGGAGAGGGCGGGGGTTCCCCTCCGTCAGTCGCCCCTGGCGCCC; CTCGCCTTGTCGCACTCTCCGCCTCGCTCTCCCCGACGTCCGGCCAGGAGGAGCCGGTAG; CATCGGGAGCCTCGCGCCGAGGGCGCCGCGGTCCGCGCCCCGCGACTGCAGCCCCCGGCC; TGGCCCCGGCGGGGCGCCCCCTCCCCTCCCCCTCCTGCGAGCTGGGATCCGGCCGGCTTC; CGCCCTCCCCTGGCCGCGAGACCGGCCCCGGCGGCTGGGCCGCCAGTAGCTCCAGCCATG; GGCTCGGGGCGCGTACCCGGGCTCTGCCTGCTTGTCCTGCTGGTCCACGCCCGCGCCGCC; CAGTACAGCAAAGCCGCGCAAGATGTGGATGAGTGTGTGGAGGGGACTGACAACTGCCAC; ATCGATGCTATCTGCCAGAACACCCCGAGGTCATACAAGTGCATCTGCAAGTCTGGCTAC; ACAGGGGACGGCAAACACTGCAAAGACGTGGATGAGTGCGAGCGAGAGGATAATGCAGGT; TGTGTGCATGACTGTGTCAACATCCCTGGCAATTACCGGTGTACCTGCTATGATGGATTC; CACCTGGCACATGACGGACACAACTGTCTGGATGTGGACGAGTGTGCCGAGGGCAACGGC; GGCTGTCAGCAGAGCTGTGTCAACATGATGGGCAGCTATGAGTGCCACTGCCGGGAAGGC; TTCTTCCTCAGCGACAACCAGCATACCTGTATCCAGCGGCCAGAAGGAATGAACTGCATG; AACAAGAACCACGGCTGTGCCCACATTTGCCGGGAGACACCCAAGGGGGGTATTGCCTGT; GAATGCCGTCCTGGCTTTGAGCTTACCAAGAACCAACGGGACTGTAAATTGACATGCAAC; TATGGTAACGGCGGCTGCCAGCACACGTGTGATGACACAGAGCAGGGTCCCCGGTGCGGC; TGCCATATCAAGTTTGTGCTCCATACCGACGGGAAGACATGCATCGAGACCTGTGCTGTC; AACAACGGGGGCTGTGACAGTAAGTGCCATGATGCAGCGATTGGTGTCCACTGCACCTGC; CCTGTGGGCTTCATGCTGCAGCCAGACAGGAAGACGTGCAAAGATATAGATGAGTGCCGC; TTAAACAACGGGGGCTGTGACCATATTTGCCGCAACACAGTGGGCAGCTTCGAATGCAGT; TGCAAGAAAGGCTATAAGCTTCTCATCAATGAGAGGAACTGCCAGGATATAGACGAGTGT; TCCTTTGATCGAACCTGTGACCACATATGTGTCAACACACCAGGAAGCTTCCAGTGTCTC; TGCCATCGTGGCTACCTGTTGTATGGTATCACCCACTGTGGGGATGTGGATGAATGCAGC; ATCAACCGGGGAGGTTGCCGCTTTGGCTGCATCAACACTCCTGGCAGCTACCAGTGTACC; TGCCCAGCAGGCCAGGGTCGGCTGCACTGGAATGGCAAAGATTGCACAGAGCCACTGAAG; TGTCAGGGCAGTCCTGGGGCCTCGAAAGCCATGCTCAGCTGCAACCGGTCTGGCAAGAAG; GACACCTGTGCCCTGACCTGTCCCTCCAGGGCCCGATTTTTGCCAGAGTCTGAGAATGGC; TTCACGGTGAGCTGTGGGACCCCCAGCCCCAGGGCTGCTCCAGCCCGAGCTGGCCACAAT; GGGAACAGCACCAACTCCAACCACTGCCATGAGGCTGCAGTGCTGTCCATTAAACAACGG; GCCTCCTTCAAGATCAAGGATGCCAAATGCCGTTTGCACCTGCGAAACAAAGGCAAAACA; GAGGAGGCTGGCAGAATCACAGGGCCAGGTGGTGCCCCCTGCTCTGAATGCCAGGTCACC; TTCATCCACCTTAAGTGTGACTCCTCTCGGAAGGGCAAGGGCCGACGGGCCCGGACCCCT; CCAGGCAAAGAGGTCACAAGGCTCACCCTGGAACTGGAGGCAGAGGTCAGAGCCGAAGAA; ACCACAGCCAGCTGTGGGCTGCCCTGCCTCCGACAGCGAATGGAACGGCGGCTGAAAGGA; TCCCTGAAGATGCTCAGAAAGTCCATCAACCAGGACCGCTTCCTGCTGCGCCTGGCAGGC; CTTGATTATGAGCTGGCCCACAAGCCGGGCCTGGTAGCCGGGGAGCGAGCAGAGCCGATG; GAGTCCTGTAGGCCCGGGCAGCACCGTGCTGGGACCAAGTGTGTCAGCTGCCCGCAGGGA; ACGTATTACCACGGCCAGACGGAGCAGTGTGTGCCATGCCCAGCGGGCACCTTCCAGGAG; AGAGAAGGGCAGCTCTCCTGCGACCTTTGCCCTGGGAGTGATGCCCACGGGCCTCTTGGA; GCCACCAACGTCACCACGTGTGCAGGTCAGTGCCCACCTGGCCAACACTCTGTAGATGGG; TTCAAGCCCTGTCAGCCATGCCCACGTGGCACCTACCAACCTGAAGCAGGACGGACCCTA; TGCTTCCCTTGTGGTGGGGGCCTCACCACCAAGCATGAAGGGGCCATTTCCTTCCAAGAC; TGTGACACCAAAGTCCAGTGCTCCCCAGGGCACTACTACAACACCAGCATCCACCGCTGT; ATTCGCTGTGCCATGGGCTCCTATCAGCCCGACTTCCGTCAGAACTTCTGCAGCCGCTGT; CCAGGAAACACAAGCACAGACTTTGATGGCTCTACCAGTGTGGCCCAATGCAAGAATCGT; CAGTGTGGTGGGGAGCTGGGTGAGTTCACTGGCTATATTGAGTCCCCCAACTACCCGGGC; AACTACCCAGCTGGTGTGGAGTGCATCTGGAACATCAACCCCCCACCCAAGCGCAAGATC; CTTATCGTGGTACCAGAGATCTTCCTGCCATCTGAGGATGAGTGTGGGGACGTCCTCGTC; ATGAGAAAGAACTCATCCCCATCCTCCATTACCACTTATGAGACCTGCCAGACCTACGAG; CGTCCCATTGCCTTCACTGCCCGTTCCAGGAAGCTCTGGATCAACTTCAAGACAAGCGAG; GCCAACAGCGCCCGTGGCTTCCAGATTCCCTATGTTACCTATGATGAGGACTATGAGCAG; CTGGTAGAAGACATTGTGCGAGATGGCCGGCTCTATGCCTCTGAAAACCACCAGGAGATT; TTAAAGGACAAGAAGCTCATCAAGGCCTTCTTTGAGGTGCTAGCCCACCCCCAGAACTAC; TTCAAGTACACAGAGAAACACAAGGAGATGCTGCCAAAATCCTTCATCAAGCTGCTCCGC; TCCAAAGTTTCCAGCTTCCTGAGGCCCTACAAATAGTAACCCTAGGCTCAGAGACCCAAT; TTTTTAAGCCCCCAGACTCCTTAGCCCTCAGAGCCGGCAGCCCCCTACCCTCAGACAAGG; AACTCTCTCCTCTCTTTTTGGAGGGAAAAAAAAAATATCACTACACAAACCAGGCACTCT; CCCTTTCTGTCTTTCTAGTTTCCTTTCCTTGTCTCTCTCTGCCTGCCTCTCTACTGTTCC; CCCTTTTCTAACACACTACCTAGAAAAGCCATTCAGTACTGGCTCTAGTCCCCGTGAGAT; GTAAAGAAACAGTACAGCCCCTTCCACTGCCCATTTTACCAGCTCACATTCCCGACCCCA; TCAGCTTGGAAGGGTGCTAGAGGCCCATCAAGGAAGTGGGTCTGGTGGGAAACGGGGAGG; GGAAAGAAGGGCTTCTGCCATTATAGGGTTGTGCCTTGCTAGTCAGGGGCCAAAATGTCC; CCTGGCTCTGCTCCCTAGGGTGATTCTAACAGCCCAGGGTCCTGCCAAAGAAGCCTTTGA; TTTACAGGCTTAATGCCAGCACCAGTCCTCTGGGGCACATGGTTTGAGCTCTGGACTTCC; CACATGGCCAGCTTTCTTGTCTATACAGATCCTCTCTTTCTTTCCCTACGTCTGCCTGGG; GTCTACTCCATAAGGGTTTACAAATGGCCCACAACACTGAGTTAGTGGACACCGGCTAAA; TGAGGAAGAGCAGCAGGCATTGTCATGGTGAATGCCCCGCTGTAGCTCCCTGAGAGAAAG; ACTGTAACTCTGCAGGACAGAAACAAGGTTTTAAAGCATTGCCAAAAAAAAAAAAAAAAA; AA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152753 unedited; NGGGCGGTTCGGATTTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCT; CTCTCCCGGCGAAGCTGGGAATTGGGTGGGATTACACGGAGCAGCCCCGCCGCCGCCGCT; GGCAGAGGCCGGCTTGGAGAGGGCGGGGGTTCCCCTCCGTCAGTCGCCCCTGGCGCCCCT; CGCCTTGTCGCACTCTCCGCCTCGCTCTCCCCGACGTCCGGCCAGGAGGAGCCGGTAGCA; TCGGGAGCCTCGCGCCGAGGGCGCCGCGGTCCGCGCCCCGCGACTGCAGCCCCCGGCCTG; GCCCCGGCGGGGCGCCCCCTCCCCTCCCCCTCCTGCGAGCTGGGATCCGGCCGGCTTCCG; CCCTCCCCTGGCCGCGAGACCGGCCCCGGCGGCTGGGCCGCCAGTAGCTCCAGCCATGGG; CTCGGGGCGCGTACCCGGGCTCTGCCTGCTTGTCCTGCTGGTCCACGCCCGCGCCGCCCA; GTACAGCAAAGCCGCGCAAGATGTGGATGAGTGTGTGGAGGGGACTGACAACTGCCACAT; CGATGCTATCTGCCAGAACACCCCGAGGTCATACAAGTGCATCTGCAAGTCTGGCTACAC; AGGGGACGGCAAACACTGCAAAGACGTGGATGAGTGCGAGCGAGAGGATAATGCAGGTTG; TGTGCATGACTGTGTCAACATCCCTGGCAATTACCGGTGTACCTGCTATGATGGATTCCA; CCTGGCACATGACGGACACAACTGTCTGGATGTGGACCAGTGTGCCGAGGGCAACGGCGG; CTGTCAGCAGAGCTGTGTCAACATGATGGGCAGCTATGAGTGCCACTTGCCGGGAAGGCT; TCTTCCTTAGCGACAACCAGCATACCCTGTATCCAGCGGCCAGAATGA;
• Restriction Sites: Please inquire
• ACCN: NM_152753
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152753.2, NP_689966.2
• RefSeq Size: 5115 bp
• RefSeq ORF: 2982 bp
• Locus ID: 222663
• UniProt ID: Q8IX30
• Cytogenetics: 6p21.31
• Protein Families: Druggable Genome, Secreted Protein
• Summary: This gene encodes a member of the signal peptide, complement subcomponents C1r/C1s, Uegf, bone morphogenetic protein-1 and epidermal growth factor-like domain containing protein family. Overexpression of this gene in human embryonic kidney cells results in secretion of a glycosylated form of the protein that forms oligomers and tethers to the cell surface. This gene is upregulated in lung cancer tumor tissue compared to healthy tissue and is associated with loss of the epithelial marker E-cadherin and with increased expression of vimentin, a mesenchymal marker. In addition, the protein encoded by this gene is a transforming growth factor beta receptor ligand, and when secreted by cancer cells, it can be cleaved in vitro to release the N-terminal epidermal growth factor-like repeat domain and the C-terminal complement subcomponents C1r/C1s domain. Both the full length protein and C-terminal fragment can bind to the transforming growth factor beta type II receptor to promote the epithelial-mesenchymal transition and tumor angiogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).