ORC1 (NM_004153) Human Untagged Clone

SKU SC126369

ORC1 (untagged)-Human origin recognition complex, subunit 1 (ORC1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: ORC1
• Synonyms: HSORC1; ORC1L; PARC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004153 edited; CTTTGGTCCTGGCTTTCGGCCCGTAGTTGTAGAAGGAGCCCTGCTGGTGCAGGTTAGAGG; TGCCGCATCCCCCGGAGCTCTCGAAGTGGAGGCGGTAGGAAACGGAGGGCTTGCGGCTAG; CCGGAGGAAGCTTTGGAGCCGGCCATGGCACACTACCCCACAAGGCTGACTACCAGAAAA; ACTTATTCATGGGTTGGCAGGCCCTTGTTGGATCGAAAACTGCACTACCAAACCTATAGA; GAAATGTGTGTGAAAACAGAAGGTTGTTCCACCGAGATTCACATCCAGATTGGACAGTTT; GTGTTGATTGAAGGGGATGATGATGAAAACCCGTATGTTGCTAAATTGCTTGAGTTGTTC; GAAGATGACTCTGATCCTCCTCCTAAGAAACGTGCTCGAGTACAGTGGTTTGTCCGATTC; TGTGAAGTCCCTGCCTGTAAACGGCATTTGTTGGGCCGGAAGCCTGGTGCACAGGAAATA; TTCTGGTATGATTACCCGGCCTGTGACAGCAACATTAATGCGGAGACCATCATTGGCCTT; GTTCGGGTGATACCTTTAGCCCCAAAGGATGTGGTACCGACGAATCTGAAAAATGAGAAG; ACACTCTTTGTGAAACTATCCTGGAATGAGAAGAAATTCAGGCCACTTTCCTCAGAACTA; TTTGCGGAGTTGAATAAACCACAAGAGAGTGCAGCCAAGTGCCAGAAACCCGTGAGAGCC; AAGAGTAAGAGTGCAGAGAGCCCTTCTTGGACCCCAGCAGAACATGTGGCCAAAAGGATT; GAATCAAGGCACTCCGCCTCCAAATCTCGCCAAACTCCTACCCATCCTCTTACCCCAAGA; GCCAGAAAGAGGCTGGAGCTTGGCAACTTAGGTAACCCTCAGATGTCCCAGCAGACTTCA; TGTGCCTCCTTGGATTCTCCAGGAAGAATAAAACGGAAAGTGGCCTTCTCGGAGATCACC; TCACCTTCTAAGAGATCTCAGCCTGATAAACTTCAAACCTTGTCTCCAGCTCTGAAAGCC; CCAGAGAAAACCAGAGAGACTGGACTCTCTTATACTGAGGATGACAAGAAGGCTTCACCT; GAACATCGCATAATCCTGAGAACCCGAATTGCAGCTTCGAAAACCATAGACATTAGAGAG; GAGAGAACACTTACCCCTATCAGTGGGGGACAGAGATCTTCAGTGGTGCCATCCGTGATT; CTGAAACCAGAAAACATCAAAAAGAGGGATGCAAAAGAAGCAAAAGCCCAGAATGAAGCG; ACCTCTACTCCCCATCGTATCCGCAGAAAGAGTTCTGTCTTGACTATGAATCGGATTAGG; CAGCAGCTTCGGTTTCTAGGTAATAGTAAAAGTGACCAAGAAGAGAAAGAGATTCTGCCA; GCAGCAGAGATTTCAGACTCTAGCAGTGACGAAGAAGAGGCTTCCACACCGCCCCTTCCA; AGGAGAGCACCCAGAACTGTGTCCAGGAACCTGCGATCTTCCTTGAAGTCATCCTTACAT; ACCCTCACGAAGGTGCCAAAGAAGAGTCTCAAGCCTAGAACGCCACGTTGTGCCGCTCCT; CAGATCCGTAGTCGAAGCCTGGCTGCCCAGGAGCCAGCCAGTGTGCTGGAGGAAGCCCGA; CTGAGGCTGCATGTTTCTGCTGTACCTGAGTCTCTTCCCTGTCGGGAACAGGAATTCCAA; GACATCTACAATTTTGTGGAAAGCAAACTCCTTGACCATACCGGAGGGTGCATGTACATC; TCCGGTGTCCCTGGGACAGGGAAGACTGCCACTGTTCATGAAGTGATACGCTGCCTGCAG; CAGGCAGCCCAAGCCAATGATGTTCCTCCCTTTCAATACATTGAGGTCAATGGCATGAAG; CTGACGGAGCCCCACCAAGTCTATGTGCAAATCTTGCAGAAGCTAACAGGCCAAAAAGCA; ACAGCCAACCATGCGGCAGAACTGCTGGCAAAGCAATTCTGCACCCGAGGGTCACCTCAG; GAAACCACCGTCCTGCTTGTGGATGAGCTCGACCTTCTGTGGACTCACAAACAAGACATA; ATGTACAATCTCTTTGACTGGCCCACTCATAAGGAGGCCCGGCTTGTGGTCCTGGCAATT; GCCAACACAATGGACCTGCCAGAGCGAATCATGATGAACCGGGTGTCCAGCCGACTGGGT; CTTACCAGGATGTGCTTCCAGCCCTATACATATAGCCAGCTGCAGCAGATCCTAAGGTCC; CGGCTCAAGCATCTAAAGGCCTTTGAAGATGATGCCATCCAGCTGGTAGCCAGGAAGGTA; GCAGCACTGTCTGGAGATGCACGACGGTGCCTGGACATCTGCAGGCGTGCCACAGAGATC; TGTGAGTTCTCCCAGCAGAAGCCTGACTCCCCTGGCCTGGTCACCATAGCCCACTCAATG; GAAGCTGTGGATGAGATGTTTTCATCATCATACATCACGGCCATCAAAAATTCCTCTGTT; CTGGAACAGAGCTTCCTGAGAGCCATCCTCGCAGAGTTCCGTCGATCAGGACTGGAGGAA; GCCACGTTTCAACAGATATATAGTCAACATGTGGCACTGTGCAGAATGGAGGGACTGCCG; TACCCCACCATGTCAGAGACCATGGCCGTGTGTTCTCACCTGGGCTCCTGTCGCCTCCTG; CTTGTGGAGCCCAGCAGGAACGATCTGCTCCTTCGGGTGCGGCTCAACGTCAGCCAGGAT; GATGTGCTGTATGCGCTGAAAGACGAGTAAAGGGGCTTCACAAGTTAAAAGACTGGGGTC; TTGCTGGGTTTTGTTTTTTGAGACGGGGTCTTGCTCTGTCGCCCAGGCTGGAGTGCAGTG; GCACGATCATGGCTCACTGCAGCCTTGACTTCTCAGGCTTAGGTGACCCCCCAACCTCAT; CCTCCCGGGTGGCTGAAACTACAGGCACATGCCACCATGCCCAGCTGATTTTTTGTAGAG; ACAGGGCTTCACCATGTTGCCAAGCTAGTCTACAAAGCATCTGATTTTGGAAGTACATGG; AATTGTTGTAACAAAGTATATTGAATGGAAATGGCTCTCATGTATTTTGGAATTTTCCAT; TAAATAATTTGCTTTTTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004153 unedited; GTTTTTATTTTGTATACCACTCATATAGGCGGCCGCGTATTCACATGCTGGTACCGGCTC; CGGTAATTCCCGGGATATCGTCGACCCACGCGTCCGCTTTGGTCCTGGCTTTCGGCCCGT; AGTTGTAGAAGGAGCCCTGCTGGTGCAGGTTAGAGGTGCCGCATCCCCCGGAGCTCTCGA; AGTGGAGGCGGTAGGAAACGGAGGGCTTGCGGCTAGCCGGAGGAAGCTTTGGAGCCGGCC; ATGGCACACTACCCCACAAGGCTGAAGACCAGAAAAACTTATTCATGGGTTGGCAGGCCC; TTGTTGGATCGAAAACTGCACTACCAAACCTATAGAGAAATGTGTGTGAAAACAGAAGGT; TGTTCCACCGAGATTCACATCCAGATTGGACAGTTTGTGTTGATTGAAGGGGATGATGAT; GAAAACCCGTATGTTGCTAAATTGCTTGAGTTGTTCGAAGATGACTCTGATCCTCCTCCT; AAGACACGTGCTCGAGTACAGTGGTTTGTCCGATTCTGTGAAGTCCCTGCCTGTAAACGG; CATTTGTTGGGCCGGAAGCCTGGTGCACAGGAAATATTCTGGTATGATTACCCGGCCTGT; GACAGCCACATTAATGCGGAGACCATCATTGGCCTTGTTCGGGTGATACCTTTAGCCCCA; AAGGATGTGGTACCGACGAATCTGAAAATGAGAAGACACTCTTTGTGAAACTATCCTGGA; ATGAGAAGAATTCAGGCCCCTTTTCTCAGAACTATTTTGCGGGTTGAATAAACCACAAGA; GAGTGCAGCCCAGTGCCAAAAACCCTGAGAGCCAGGATTAAAGTGCAAAGAGCCTTCTTG; GACCCCCCAAAACTGGTGCCCAAGGATGGATCAAGGCACTCCGCTTCAAATTTGGCCAAA; TTCTACCCATCCTTTACCCAAGA;
• Restriction Sites: Please inquire
• ACCN: NM_004153
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004153.2, NP_004144.2
• RefSeq Size: 3180 bp
• RefSeq ORF: 2586 bp
• Locus ID: 4998
• UniProt ID: Q13415
• Cytogenetics: 1p32.3
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Cell cycle
• Summary: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]; Transcript Variant: This variant (1) is the longest transcript and encodes the longer isoform (1).