FMO2 (NM_001460) Human Untagged Clone

SKU SC126279

FMO2 (untagged)-Human flavin containing monooxygenase 2 (non-functional) (FMO2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FMO2
• Synonyms: FMO1B1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001460 edited; ACAACCAAGGGAGAAAACTATTCTGTCAAAGAGACGGTGCCAAAAGGCAAAAACAAAGGA; GCTGATGGCAAAGAAGGTAGCTGTGATTGGAGCTGGGGTCAGTGGCCTAATTTCTCTGAA; GTGCTGTGTGGATGAGGGACTTGAGCCCACTTGCTTTGAGAGAACTGAAGATATTGGAGG; AGTGTGGAGGTTCAAAGAGAATGTGGAAGATGGCCGAGCAAGTATCTATCAATCTGTCGT; TACCAACACCAGCAAAGAAATGTCCTGTTTCAGTGACTTTCCAATGCCTGAAGATTTTCC; AAACTTCCTGCATAATTCTAAACTTCTGGAATATTTCAGGATTTTTGCTAAAAAATTTGA; TCTGCTAAAATATATTCAGTTCCAGACAACTGTCCTTAGTGTGAGAAAATGTCCAGATTT; CTCATCCTCTGGCCAATGGAAGGTTGTCACTCAGAGCAACGGCAAGGAGCAGAGTGCTGT; CTTTGACGCAGTTATGGTTTGCAGTGGCCACCACATTCTACCTCATATCCCACTGAAGTC; ATTTCCAGGTATGGAGAGGTTCAAAGGCCAATATTTCCATAGCCGCCAATACAAGCATCC; AGATGGATTTGAGGGAAAACGCATCCTGGTGATTGGAATGGGAAACTCAGGCTCAGATAT; TGCTGTTGAGCTGAGTAAGAATGCTGCTCAGGTTTTTATCAGCACCAGGCATGGCACCTG; GGTCATGAGCCGTATCTCTGAAGATGGCTATCCTTGGGACTCAGTGTTCCACACCCGGTT; TCGTTCTATGCTCCGCAATGTACTGCCACGAACAGCTGTAAAATGGATGATAGAACAACA; GATGAATCGGTGGTTCAACCATGAAAATTATGGCCTTGAGCCTCAAAACAAATACATTAT; GAAGGAACCTGTACTAAATGATGATGTCCCAAGTCGTCTACTCTGTGGAGCCATCAAGGT; GAAATCTACAGTGAAAGAGCTCACAGAAACTTCTGCCATCTTTGAGGATGGAACAGTGGA; GGAGAACATTGATGTCATCATTTTTGCAACAGGATATAGTTTCTCTTTTCCCTTCCTTGA; AGATTCACTCGTTAAAGTAGAGAATAATATGGTCTCACTGTATAAATACATATTCCCCGC; TCACCTGGACAAGTCAACCCTCGCGTGCATTGGTCTCATCCAGCCCCTAGGTTCCATTTT; CCCAACTGCTGAACTTCAAGCTCGTTGGGTGACAAGAGTTTTCAAAGGCTTGTGTAGCCT; GCCCTCAGAGAGAACTATGATGATGGACATTATCAAAAGGAATGAAAAAAGAATTGACCT; GTTTGGAGAAAGCCAGAGCCAGACGTTGCAGACCAATTATGTTGACTACTTGGACGAGCT; CGCCTTAGAGATAGGTGCGAAGCCAGATTTCTGCTCTCTCTTGTTCAAAGATCCTAAACT; GGCTGTGAGACTCTATTTCGGACCCTGCAACTCCTATTAGTATCGCCTGGTTGGGCCTGG; GCAATGGGAAGGAGCCAGAAATGCCATCTTCACCCAGAAACAAAGAATACTGAAGCCACT; CAAGACTCGGGCCCTGAAGGATTCATCTAATTTCTCAGTTTCTTTTCTGTTGAAAATCCT; GGGCCTTCTTGCTGTTGTTGTGGCCTTTTTTTGCCAACTTCAATGGTCCTAGTCAGCATA; ATGCTTTGGGCTTTATTATCTTGTCAGTCACTACCTCCTAAAAAAAAAAAAAAAAAAAAA; AAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001460 unedited; GTTCAGGAATTTGTAATACGACTCCACTATAGGCGGCCGCGCAATTCGCCATTACGGCCG; GGGACAACCAAGGAGAAAACTATTGTGTCAAAGAACGGTGCCAAAAGGCAAAAACAAAGG; AGCTGATGGCAAAGAAGGTAGCTGTGATTGGAGCTGGGGTCAGTGGCCTAATTTCTCTGA; AGTGCTGTGTGGATGAGGGACTTGAGCCCACTTGCTTTGAGAGAACTGAAGATATTGGAG; GAGTGTGGAGGTTCAAAGAGAATGTGGAAGATGGCCGAGCAAGTATCTATCAATCTGTCG; TTACCAACACCAGCAAAGAAATGTCCTGTTTCAGTGACTTTCCAATGCCTGAAGATTTTC; CAAACTTCCTGCATAATTCTAAACTTCTGGAATATTTCAGGAGTTTTGCTAAAAAATTTG; ATCTGCTAAAATATATTCAGTTCCAGACAACTGTCCTTAGTGTGAGAAAATGTCCAGATT; TCTCATCCTCTGGCCAATGGAAGGTTGTCACTCAAAGCAACGGCAAGGAGCAGAGTGCTG; TCTTTGACGCAGTTATGGTTTGCAGTGGCCACCACATTCTACCTCATATCCCACTGAAGT; CATTTCCAGGTATGGAGAGGTTCAAAGGCCAATATTTCCATAGCCGCCAATACAAGCATC; CAGATGGATTTGAGGGAAAACGCATCCTGGTGATTGGAATGGGAAACTCANGCTCANATA; TTGCTGTTGAGCTGAGTAAGAATGCTGCTCANGTGTTTATCAGCACCAGCATGGCACCTG; GGTCATGAGCCCTATCTCTGAGATGGCTATCCTTGGGACTCANTGTTCCACACCGGTTTC; GTTCATGCTCCGCATGTACTGCCACAACAGCTGTAAAATGGTGATAGGACAACGATGAAT; CGTTGGTCAACCTGAAAATATGGGCCTTAGCCTCAAACAATACTTATGA;
• Restriction Sites: Please inquire
• ACCN: NM_001460
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001460.2, NP_001451.1
• RefSeq Size: 5181 bp
• RefSeq ORF: 1416 bp
• Locus ID: 2327
• UniProt ID: Q99518
• Cytogenetics: 1q24.3
• Protein Pathways: Drug metabolism - cytochrome P450
• Summary: This gene encodes a flavin-containing monooxygenase family member. It is an NADPH-dependent enzyme that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, some human populations contain an allele (FMO2*2A) with a premature stop codon, resulting in a protein that is C-terminally-truncated, has no catalytic activity, and is likely degraded rapidly. This gene is found in a cluster with other related family members on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]; Transcript Variant: This variant (1), which represents the functional FMO2*1 allele, is the longer transcript and encodes the longer isoform (a).