XRCC4 (NM_022550) Human Untagged Clone

SKU SC126125

XRCC4 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 4 (XRCC4), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: XRCC4
• Synonyms: SSMED
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_022550 edited; GGGGACCGGAAGTAGAGTCACGGAGAGGTAGGATCCGGAAGTGGGGCTGCCTCTTTAAAT; AACAAAAATCTGAGGTATTAAGAAATGGAGAGAAAAATAAGCAGAATCCACCTTGTTTCT; GAACCCAGTATAACTCATTTTCTACAAGTATCTTGGGAGAAAACACTGGAATCTGGTTTT; GTTATTACACTTACTGATGGTCATTCAGCATGGACTGGGACAGTTTCTGAATCAGAGATT; TCCCAAGAAGCTGATGACATGGCAATGGAAAAAGGGAAATATGTTGGTGAACTGAGAAAA; GCATTGTTGTCAGGAGCAGGACCAGCTGATGTATACACGTTTAATTTTTCTAAAGAGTCT; TGTTATTTCTTCTTTGAGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCTTCAAC; CTAGAGAAAGTTGAAAACCCAGCTGAAGTCATTAGAGAACTTATTTGTTATTGCTTGGAC; ACCATTGCAGAAAATCAAGCCAAAAATGAGCACCTGCAGAAAGAAAATGAAAGGCTTCTG; AGAGATTGGAATGATGTTCAAGGACGATTTGAAAAATGTGTGAGTGCTAAGGAAGCTTTG; GAGACTGATCTTTATAAGCGGTTTATTCTGGTGTTGAATGAGAAGAAAACAAAAATCAGA; AGTTTGCATAATAAATTATTAAATGCAGCTCAAGAACGAGAAAAGGACATCAAACAAGAA; GGGGAAACTGCAATCTGTTCTGAAATGACTGCTGACCGAGATCCAGTCTATGATGAGAGT; ACTGATGAGGAAAGTGAAAACCAAACTGATCTCTCTGGGTTGGCTTCAGCTGCTGTAAGT; AAAGATGATTCCATTATTTCAAGTCTTGATGTCACTGATATTGCACCAAGTAGAAAAAGG; AGACAGCGAATGCAAAGAAATCTTGGGACAGAACCTAAAATGGCTCCTCAGGAGAATCAG; CTTCAAGAAAAGGAAAATTCTAGGCCTGATTCTTCACTACCTGAGACGTCTAAAAAGGAG; CACATCTCAGCTGAAAACATGTCTTTAGAAACTCTGAGAAACAGCAGCCCAGAAGACCTC; TTTGATGAGATTTAACAGTCTCAAAAAATACTTTGATGTTCACTAGACTATGTTTTCTAT; TCATTTCTTTAAAATGAAAAAGGAGAATTTCAAGTCAGCAGCCGCTATTACCGTATCTTA; CAATTTAATTACATACACAGTGAATTGAAACCATTGTGCAAAATGGATTACACATGTATA; CAAAGATACGATTTGATGATGACACTGGCACATTATTCTAAACTATTCATTCAGCATGCC; TATAATTACATAAATTGTATGAGACTTTTTGTTGCAAAGGACACATTTATCATATTCATT; CACACATATTATATGTGATAGCTGTCCAACATCCTGTCTGGGAAGATTTTGAAAACAGGA; CAAAGAAAACATCACTTTAAAATGTCTTCAGCTTTTTTTGAATAGACGTATTCAAACATA; TTCTGAACATTGATGTTTGAACATTTTAATTTGTGTGATGATGTAGAAAATATAATTTTA; GTTTGTACATAAACATTGTGAAAATCTGATAATAAAATTTTTGATACACTAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022550 unedited; CCCGCGGTTCAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGCAATTCGCCATTA; CGGCCGGGGACCGCGAAGTAGATCACGGAGGGGTGGGATCCGGGAGTGGGGCTGCCTCTT; TAAATAACAAAAATCTGAGGTATTAAGAAATGGAGAGAAAAATAAGCAGAATCCACCTTG; TTTCTGAACCCAGTATAACTCATTTTCTACAAGTATCTTGGGAGAAAACACTGGAATCTG; GTTTTGTTATGACACTTACTGATGGTCATTCAGCATGGACTGGGACAGTTTCTGAATCAG; AGATTTCCCAAGAAGCTGATGACATGGCAATGGAAAAAGGGAAATATGTTGGTGAACTGA; GAAAAGCATTGTTGTCAGGAGCAGGACCAGCTGATGTATACACGTTTAATTTTTCTAAAG; AGTCTTGTTATTTCTTCTTTGAGAAAAACCTGAAAGATGTCTCATTCAGACTTGGTTCCT; TCAACCTAGAGAAAGTTGAAAACCCAGCTGAAGTCATTAGAGAACTTATTTGTTATTGCT; TGGACACCATTGCAGAAAATCAAGCCAAAAATGAGCACCTGCAGAAAGAAAATGAAAGGC; TTCTGAGAGATTGGAATGATGTTCAAGGACGATTTGAAAAATGTGTGAGTGCTAAGGAAG; CTTTGGAGACTGATCTTTATAAGCGGTTTATTCTGGTGTTGAATGAGAAGAANACAAAAT; CAGAAGTTTGCATAATAAATTATTAAATGCAGCTCAAGGACGAGGAAAAGACATCAAACA; AGAAGGGGAAACTGCAATCTGTTCTGAAATGACTGCTGACCGAGTCCAGTCTATGATGAG; AGTACTGATGAGGGAAGTGAAAACCAACTGATCTCTCTGGGGTGGCTTCAGCTGCTGTAA; GTAAGATAN;
• Restriction Sites: Please inquire
• ACCN: NM_022550
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022550.1, NP_072044.1
• RefSeq Size: 1707 bp
• RefSeq ORF: 1005 bp
• Locus ID: 7518
• UniProt ID: Q13426
• Cytogenetics: 5q14.2
• Protein Families: Druggable Genome
• Protein Pathways: Non-homologous end-joining
• Summary: The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternate transcript variants such as NM_022406 are unlikely to be expressed in some individuals due to a polymorphism (rs1805377) in the last splice acceptor site. [provided by RefSeq, Oct 2019]; Transcript Variant: This variant (3) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1 and 3 encode the same isoform (1).