EXDL1 (EXD1) (NM_152596) Human Untagged Clone

SKU SC126090

EXD1 (untagged)-Human exonuclease 3'-5' domain containing 1 (EXD1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: EXDL1
• Synonyms: EXDL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_152596 edited; GCGTACCTTTCTCCAGGTTTTCAGCCTGTGATTTGATAACCGCCGCCGGGTGGCGTGGAG; CGTCCCGAGACTTCTTCCTTCATTGCAGCCCCTCTTCTTTTAGTTCTAGACTGAAGTTCG; AGGAACGCACCACTTGGCTGAAATTAGGATCCAGATTTCCCAAACTTCCTTCGGAGGAAA; AAGCCCTAACGATGGAGGACAGTGAATTCCTAGCTTATGTGGAACTACTAGATGAAGTGG; AACAAGGCTCAGTGAGAGCAAAAGCATCTTCTGTTAGTCTACATGCAGAAAGAACCTGGA; TGGAGAAAATGAAAGTTGAAGACCTAAATGTATGTGAGCCTGCTTCTCCTGCCCCTGAAG; CACCAGCTACCTCTCTGCTGAATGACCTCAAGTACAGCCCATCAGAGGAAGAGGAGGTGA; CATACACAGTCATTAATCAATTCCAGCAGAAGTTTGGTGCTGCGATACTCCATATCAAGA; AGCAGAATGTCCTGAGTGTGGCAGCAGAAGGAGCGAATGTATGTCGCCATGGCAAACTGT; GCTGGCTGCAGGTGGCCACAAATTGCCGAGTTTACTTATTTGACATTTTCCTTCTGGGAA; GTCGAGCTTTCCACAATGGACTTCAGATGATACTAGAAGACAAGAGAATTTTGAAGGTTA; TCCATGATTGTCGTTGGCTTTCTGATTGCCTCTCTCATCAGTATGGAATTTTGCTGAATA; ATGTCTTTGACACACAGGTAGCAGATGTACTTCAGTTTTCCATGGAAACGGGTGGCTATC; TTCCAAACTGCATCACTACTTTGCAGGAGAGTTTAATCAAACACCTTCAAGTAGCCCCTA; AATATCTCTCCTTTCTAGAAAAGAGACAAAAACTAATTCAGGAAAATCCAGAAGTATGGT; TCATCCGACCTGTTTCACCCTCTTTACTGAAAATTTTGGCCCTGGAAGCTACCTACCTGT; TACCCCTTCGCTTGGCACTCCTAGATGAGATGATGTCTGACCTAACCACCCTGGTGGATG; GTTACCTAAACACGTATCGCGAAGGGTCTGCAGACCGGCTTGGAGGCACTGAGCCTACAT; GTATGGAGCTGCCAGAGGAACTGCTTCAACTCAAGGACTTCCAGAAGCAGCGCAGGGAGA; AAGCTGCAAGAGAATATAGGGTGAATGCACAGGGACTCCTGATAAGGACAGTGCTACAGC; CAAAGAAATTAGTGACAGAGACAGCAGGGAAAGAGGAGAAAGTCAAAGGCTTCTTATTTG; GTAAAAATTTTAGGATAGATAAAGCTCCAAGTTTTACATCTCAAGACTTTCACGGGGATG; TGAATTTACTGAAAGAAGAATCTTTGAATAAACAAGCTACAAATCCTCAACATCTACCTC; CCACGGAGGAAGGGGAAACTAGTGAGGATTCCAGTAACAAACTCATTTGCACAAAGTCAA; AGGGGTCAGAGGACCAGAGAATAACTCAGAAAGAACACTTTATGACACCCAAACATGAGT; TTCAGGCAAGTTTATCTTTGAAAGAGGAGACAGAACAGTTATTGATGGTGGAAAACAAGG; AAGATTTAAAATGCACAAAACAGGCTGTTTCAATGTCTTCCTTTCCTCAGGAAACCAGAG; TGTCTCCAAGTGACACTTTTTATCCTATCAGAAAGACTGTGGTTTCCACACTCCCTCCCT; GTCCAGCCTTGGAGAAGATCGATTCCTGGATAAGTCCTTTTCTAAATCTGCCCTAGAGAT; GGGCAGTTTGTTCTTAAGGCCATGCAGATGGCTTATTTCCTTTGCCATCAGGGCTTTCCA; CAGTGCCCAGGTTTCTCATGTTGTAAATGTAGTAATGCTTCAGTCACAGGGGAAAATTAT; ATCCTCTTGCTTACTCCTGTGTTCCTGGTATGCGGAGAATGAGAATGAATAAGTTAAATA; ATGGAAGAAGTATAATTTCTGATTATGTCACTGTGTAGAAATGTTCTCAGTGACCAGAGT; GCATTATTTTTCATAATTTGGGTTTAGAGGATTTGAAGAAAGGAAGAATCTTGGGCTTAG; TATCAGGAAGACTCCATCATTTTCAAATTTTGTTTTGCTTCTTGACTTTTGGATTCCTTT; GAAGAGACCTGGTAAAACTATTAACAATTCATTTAAAAAATTGGTACCTGATAACTTTAC; CAGTACTTTTTTCCTTTTTATTTATTTGTTTTATTTATTTTTTTTTACCGCTCCTTGTGG; AGCAGGGCTACACCATAGGCAGTGTGCCCAGAGTAACCACTTTTTTCCTTTTTAAAATAT; AATATTAACTTTATGTTTGAATGTTGAATGTTTTGTCTGTCTCTTAGGCAAATAATGTTA; TAGGAATCAATAATTTAATTTTTGTTTTATTTGTTTTTTGATGGAGTCTCACTCTGTCAC; CCAGGCTGGAGTGTAGTGGTGTAATCTCTGCTCACTGCAACCTCCGCCTCCCGGGTTCAA; GCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCGTGCCACCACGCCC; GGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCG; AACTCCTGACTGACCTTGTGGTCCACCTGCCTTGGCCTTCCAAAGTGCTGGGATTACAGG; TGTGAGGCACGGTGCCCAGCCAATATCTTTATTTTAATTTGTTTTTATTTCCTTTATTTT; TAGCTGGTTTTGTCCATTTTCCTAACAAAGCAGGGACCCTGGGTTTCTTTTTAGTCTGTC; TGTTATATAAACTTGAAGCCTGACTCCATTCTATTTGCCTGGAGTTAGTATACTTTCTTA; GGGTGAAAGGAAGGCAGCTTGTATTGAGCCTTTTAAAGTATTGAATGCTTGCAAATTGCT; AACATTCTTTTGTGTAAAATAACCAATAAACCTGTTTTGTCATACTCTACTTAAAAAAAA; AAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152596 unedited; GGTCGGATTTGTATACGACTCATATAGGCGGCCGCATAACTTCGTATAGCATACATTATA; CGAAGTTATGGATCAGGCCAAATCGGCCGAGCTCGAATTCGTCGAGAGCGGGCGTACCTT; TCTCCAGGTTTTCAGCCTGTGATTTGATAACCGCCGCCGGGTGGCGTGGAGCGTCCCGAG; ACTTCTTCCTTCATTGCAGCCCCTCTTCTTTTAGTTCTAGACTGAAGTTCGAGGAACGCA; CCACTTGGCTGAAATTAGGATCCAGATTTCCCAAACTTCCTTCGGAGGAAAAAGCCCTAA; CGATGGAGGACAGTGAATTCCTAGCTTATGTGGAACTACTAGATGAAGTGGAACAAGGCT; CAGTGAGAGCAAAAGCATCTTCTGTTAGTCTACATGCAGAAAGAACCTGGATGGAGAAAA; TGAAAGTTGAAGACCTAAATGTATGTGAGCCTGCTTCTCCTGCCCCTGAAGCACCAGCTA; CCTCTCTGCTGAATGACCTCAAGTACAGCCCATCAGAGGAAGAGGAGGTGACATACACAG; TCATTAATCAATTCCAGCAGAAGTTTGGTGCTGCGATACTCCATATCAAGAAGCAGAATG; TCCTGAGTGTGGCAGCAGAAGGAGCGAATGTATGTCGCCATGGCAAACTGTGCTGGCTGC; AGGTGGCCACAAATTGCCGAGTTTACTTATTTGACATTTTCCTTCTGGGAAGTCGAGCTT; TCCACAATGGACTTCAGATGATACTAGAAGACAAGAGAATTTTGAAGGTTATCCATGATT; GTCGTTTGCTTTCTGATTGCCTCTCTCATCAGTATGGAATTTTGCTGAATAATGTCTTTG; ACACACGGTAGCANATGTACTTCAGTTTTTCATGGAAAACGGTGGCTATCTTCCAAACTG; CATCACTACTTTGCAGGAGAGTTTAATCAACCACCTTCAGTAGCCCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_152596
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152596.2, NP_689809.2
• RefSeq Size: 2949 bp
• RefSeq ORF: 1545 bp
• Locus ID: 161829
• UniProt ID: Q8NHP7
• Cytogenetics: 15q15.1
• Summary: RNA-binding component of the PET complex, a multiprotein complex required for the processing of piRNAs during spermatogenesis. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposable elements, preventing their mobilization, which is essential for the germline integrity (By similarity). The PET complex is required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs. In the PET complex, EXD1 probably acts as an RNA adapter. EXD1 is an inactive exonuclease (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.