Telomerase reverse transcriptase (TERT) (NM_198253) Human Untagged Clone
$1,479.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | Telomerase reverse transcriptase |
| Synonyms | CMM9; DKCA2; DKCB4; EST2; hEST2; hTRT; PFBMFT1; TCS1; TP2; TRT |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_198253 |
| Insert Size | 4000 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_198253.2, NP_937983.2 |
| RefSeq Size | 4018 bp |
| RefSeq ORF | 3399 bp |
| Locus ID | 7015 |
| UniProt ID | O14746 |
| Cytogenetics | 5p15.33 |
| Protein Families | Druggable Genome |
| Summary | Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. provided by RefSeq, Jul 2008 Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
"NM_198253" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC217436 | TERT (Myc-DDK-tagged)-Human telomerase reverse transcriptase (TERT), transcript variant 1 | 10 ug |
$589.00
$985.00
|
|
| RC217436L1 | Lenti ORF clone of Human telomerase reverse transcriptase (TERT), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,285.00
|
|
| RC217436L2 | Lenti ORF clone of Human telomerase reverse transcriptase (TERT), transcript variant 1, mGFP tagged | 10 ug |
$1,285.00
|
|
| RC217436L3 | Lenti ORF clone of Human telomerase reverse transcriptase (TERT), transcript variant 1, Myc-DDK-tagged | 10 ug |
$1,285.00
|
|
| RC217436L4 | Lenti ORF clone of Human telomerase reverse transcriptase (TERT), transcript variant 1, mGFP tagged | 10 ug |
$1,285.00
|
|
| RG217436 | TERT (tGFP-tagged) - Human telomerase reverse transcriptase (TERT), transcript variant 1 | 10 ug |
$789.00
$1,185.00
|
|
Citations
MicroRNA-532 and microRNA-3064 inhibit cell proliferation and invasion by acting as direct regulators of human telomerase reverse transcriptase in ovarian cancer
Bai, Wang, Wang et al
PLoS ONE (2017) 12 (3), e0173912 DOI: 10.1371/journal.pone.0173912
Bai, Wang, Wang et al
PLoS ONE (2017) 12 (3), e0173912 DOI: 10.1371/journal.pone.0173912
AI Summary: OriGene’s hTERT cDNA plasmids were used in this study to overexpress hTERT and assess how microRNA-532 and microRNA-3064 regulate hTERT-driven proliferation and invasion in ovarian cancer cells.
Snippet: following the manufacturer's protocol. Transfection of hTERT cDNA plasmids (OriGene, MD, USA) were also performed using Lipofectamine 2000 (Invitrogen, CA, USA). Real-time quantitative RT-PCR (qPCR). The total RNA was ... All 4 versions Cite SaveSaving
Selective induction of alternatively spliced FynT isoform by TNF facilitates persistent inflammatory responses in astrocytes
Lee, Low, Wong et al
Sci Rep (2017) 7, 43651 DOI: 10.1038/srep43651
Lee, Low, Wong et al
Sci Rep (2017) 7, 43651 DOI: 10.1038/srep43651
AI Summary: OriGene’s pCMV6 Entry vector containing the FynT-WT open reading frame with a C-terminal Myc-FLAG tag was used as the template to generate constitutively active and kinase-dead FynT mutants, enabling the study of FynT’s role in astrocyte inflammatory responses in this paper.
Snippet: pCMV6 Entry vector carrying the open reading frame of FynT-WT with C-terminal tagged of Myc-FLAG (OriGene Technologies) was subjected to site-directed mutagenesis to generate the constitutively active (CA) (FynT-Y528F, with an A/T switch at position 1583 resulting ... All 4 versions Cite SaveSaving
A novel method for banking stem cells from human exfoliated deciduous teeth: lentiviral TERT immortalization and phenotypical analysis
Yin, Wang, Li et al
Stem Cell Res Ther (2016) 7, 50 DOI: 10.1186/s13287-016-0309-0
Yin, Wang, Li et al
Stem Cell Res Ther (2016) 7, 50 DOI: 10.1186/s13287-016-0309-0
Cited by 30
AI Summary: In this study, the pCMV6-XL5 plasmid from OriGene, containing the full-length human TERT cDNA, was used to generate lentiviral vectors for the stable immortalization of stem cells from human exfoliated deciduous teeth (SHED), enabling extended cell proliferation and analysis.
Snippet: Cloning of TERT in lentiviral expression plasmid and lentiviral production. pCMV6-XL5 plasmid (OriGene Technologies, Beijing, China) containing full-length cDNA of human TERT (3.6 kb) [Genbank:NM_198253.2] was amplified in DH5α E. coli strain. ... Cite SaveSaving
Showing 1-3 of 3 papers.
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