DUSP4 (NM_057158) Human Untagged Clone

SKU SC125374

DUSP4 (untagged)-Human dual specificity phosphatase 4 (DUSP4), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: DUSP4
• Synonyms: HVH2; MKP-2; MKP2; TYP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125374 sequence for NM_057158 edited (data generated by NextGen Sequencing); ATGGGAAGAAAAGTTCACTCCAACGGAAGCCAGTTTGCTGAACATAGCAGATCGCCCAGG; AGGACTGGGAGAGACTGCAAACCAGTTCGAGCCCCCAGCATGGCGTTAGGTGTCAGCCAG; CTGGCAGGAAGGTCCAGGTGTCTGTGTTCAGAGTCTCAAGGCGGCTATGAGAGGTTTTCC; TCCGAGTACCCAGAATTCTGTTCTAAAACCAAGGCCCTGGCAGCCATCCCACCCCCGGTT; CCCCCCAGTGCCACAGAGCCCTTGGACCTGGGCTGCAGCTCCTGTGGGACCCCACTACAC; GACCAGGGGGGTCCTGTGGAGATCCTTCCCTTCCTCTACCTCGGCAGTGCCTACCATGCT; GCCCGGAGAGACATGCTGGACGCCCTGGGCATCACGGCTCTGTTGAATGTCTCCTCGGAC; TGCCCAAACCACTTTGAAGGACACTATCAGTACAAGTGCATCCCAGTGGAAGATAACCAC; AAGGCCGACATCAGCTCCTGGTTCATGGAAGCCATAGAGTACATCGATGCCGTGAAGGAC; TGCCGTGGGCGCGTGCTGGTGCACTGCCAGGCGGGCATCTCGCGGTCGGCCACCATCTGC; CTGGCCTACCTGATGATGAAGAAACGGGTGAGGCTGGAGGAGGCCTTCGAGTTCGTTAAG; CAGCGCCGCAGCATCATCTCGCCCAACTTCAGCTTCATGGGGCAGCTGCTGCAGTTCGAG; TCCCAGGTGCTGGCCACGTCCTGTGCTGCGGAGGCTGCTAGCCCCTCGGGACCCCTGCGG; GAGCGGGGCAAGACCCCCGCCACCCCCACCTCGCAGTTCGTCTTCAGCTTTCCGGTCTCC; GTGGGCGTGCACTCGGCCCCCAGCAGCCTGCCCTACCTGCACAGCCCCATCACCACCTCT; CCCAGCTGTTAG; ; Clone variation with respect to NM_057158.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_057158 unedited; AATACGACTCACTATAGGGCGGCCGCGAATTCGCCCTTCTAACGAAGAGGAAGCACGATG; GGAAGAAAAGTTCACTCCAACGGAAGCCAGTTTGCTGAACATAGCAGATCGCCCAGGAGG; ACTGGGAGAGACTGCAAACCAGTTCGAGCCCCCAGCATGGCGTTAGGTGTCAGCCAGCTG; GCAGGAAGGTCCAGGTGTCTGTGTTCAGAGTCTCAAGGCGGCTATGAGAGGTTTTCCTCC; GAGTACCCAGAATTCTGTTCTAAAACCAAGGCCCTGGCAGCCATCCCACCCCCGGTTCCC; CCCAGTGCCACAGAGCCCTTGGACCTGGGCTGCAGCTCCTGTGGGACCCCACTACACGAC; CAGGGGGGTCCTGTGGAGATCCTTCCCTTCCTCTACCTCGGCAGTGCCTACCATGCTGCC; CGGAGAGACATGCTGGACGCCCTGGGCATCACGGCTCTGTTGAATGTCTCCTCGGACTGC; CCAAACCACTTTGAAGGACACTATCAATACAAGTGCATNCCAGTTGAAAGATAACCACAN; GGCCGACTTCAACTCCTGTTCATTGGANGCAATANAGTACATCAATGCCCTNAAGGACTG; GCCTGGCCNCTTCCTGGTTCCATTGCCCAGCCGGCTATCTCGGGGTCGGCACCTTCTGCC; TGCCCTAACTGGTGATGAAAAACCGGTGAGCTTGGAGAAGCCTTCGAGTTGTTAACAAAC; CCGAACATTTTTTTGGCCAATTTAGTTTGT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_057158 unedited; ACTTCAGGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTC; AGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGATATCGGTACCAGA; TCTGAATTCGCCCTTCTGCTCAGTCCCAACTTTCTGCCCGCATGCGGCCCGTCCTACCCT; TGCTGGGAGCCAGCTCTGGTTCTGGGGCCCCCAGGGCGGCTCTAACAGCTGGGAGAGGTG; GTGATGGGGCTGTGCAGGTAGGGCAGGCTGCTGGGGGCCGAGTGCACGCCCACGGAGACC; GGAAAGCTGAAGACGAACTGCGAGGTGGGGGTGGCGGGGGTCTTGCCCCGCTCCCGCAGG; GGTCCCGAGGGGCTAGCAGCCTCCGCAGCACAGGACGTGGCCAGCACCTGGGACTCGAAC; TGCAGCAGCTGCCCCATGAAGCTGAAGTTGGGCGAGATGATGCTGCGGCGCTGCTTAACG; AACTCGAAGGCCTNCTCCAGCCTCACCGGTTCTTCATCATCAAGTAGGCCCAGCANATGG; TGGCCCGACGCCAAGATGCCCGCCTGGCAATTGCACAACACGCCCCCCACGGAGTTCCTT; CACGGCATTCAGGTACTCTATGCTTCCATGAACCAGAACTGATGTCGGCCTTGGGTTATC; TCCCTGAAAGCCATTGTGCCGGTAGTGCCTCAAAGGGTTGGGCATCCGAGGAACTTAACA; AACCGTGAGCCAAGGCTC;
• Restriction Sites: Please inquire
• ACCN: NM_057158
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_057158.2, NP_476499.1
• RefSeq Size: 3404 bp
• RefSeq ORF: 912 bp
• Locus ID: 1846
• UniProt ID: Q13115
• Cytogenetics: 8p12
• Protein Families: Phosphatase
• Protein Pathways: MAPK signaling pathway
• Summary: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.