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VCP (NM_007126) Human Untagged Clone

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SKU
SC125280
VCP (untagged)-Human valosin containing protein (VCP)
  $739.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol VCP
Synonyms CDC48; FTDALS6; p97; TERA
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC125280 sequence for NM_007126 edited (data generated by NextGen Sequencing)
ATGGCTTCTGGAGCCGATTCAAAAGGTGATGACCTATCAACAGCCATTCTCAAACAGAAG
AACCGTCCCAATCGGTTAATTGTTGATGAAGCCATCAATGAGGACAACAGTGTGGTGTCC
TTGTCCCAGCCCAAGATGGATGAATTGCAGTTGTTCCGAGGTGACACAGTGTTGCTGAAA
GGAAAGAAGAGACGAGAAGCTGTTTGCATCGTCCTTTCTGATGATACTTGTTCTGATGAG
AAGATTCGGATGAATAGAGTTGTTCGGAATAACCTTCGTGTACGCCTAGGGGATGTCATC
AGCATCCAGCCATGCCCTGATGTGAAGTACGGCAAACGTATCCATGTGCTGCCCATTGAT
GACACAGTGGAAGGCATTACTGGTAATCTCTTCGAGGTATACCTTAAGCCGTACTTCCTG
GAAGCGTATCGACCCATCCGGAAAGGAGACATTTTTCTTGTCCGTGGTGGGATGCGTGCT
GTGGAGTTCAAAGTGGTGGAAACAGATCCTAGCCCTTATTGCATTGTTGCTCCAGACACA
GTGATCCACTGCGAAGGGGAGCCTATCAAACGAGAGGATGAGGAAGAGTCCTTGAATGAA
GTAGGGTATGATGACATTGGTGGCTGCAGGAAGCAGCTAGCTCAGATAAAGGAGATGGTG
GAACTGCCCCTGAGACATCCTGCCCTCTTTAAGGCAATTGGTGTGAAGCCTCCTAGAGGA
ATCCTGCTTTACGGACCTCCTGGAACAGGAAAGACCCTGATTGCTCGAGCTGTAGCAAAT
GAGACTGGAGCCTTCTTCTTCTTGATCAATGGTCCTGAGATCATGAGCAAATTGGCTGGT
GAGTCTGAGAGCAACCTTCGTAAAGCCTTTGAGGAGGCTGAGAAGAATGCTCCTGCCATC
ATCTTCATTGATGAGCTAGATGCCATCGCTCCCAAAAGAGAGAAAACTCATGGCGAGGTG
GAGCGGCGCATTGTATCACAGTTGTTGACCCTCATGGATGGCCTAAAGCAGAGGGCACAT
GTGATTGTTATGGCAGCAACCAACAGACCCAACAGCATTGACCCAGCTCTACGGCGATTT
GGTCGCTTTGACAGGGAGGTAGATATTGGAATTCCTGATGCTACAGGACGCTTAGAGATT
CTTCAGATCCATACCAAGAACATGAAGCTGGCAGATGATGTGGACCTGGAACAGGTAGCC
AATGAGACTCACGGGCATGTGGGTGCTGACTTAGCAGCCCTGTGCTCAGAGGCTGCTCTG
CAAGCCATCCGCAAGAAGATGGATCTCATTGACCTAGAGGATGAGACCATTGATGCCGAG
GTCATGAACTCTCTAGCAGTTACTATGGATGACTTCCGGTGGGCCTTGAGCCAGAGTAAC
CCATCAGCACTGCGGGAAACCGTGGTAGAGGTGCCACAGGTAACCTGGGAAGACATCGGG
GGCCTAGAGGATGTCAAACGTGAGCTACAGGAGCTGGTCCAGTATCCTGTGGAGCACCCA
GACAAATTCCTGAAGTTTGGCATGACACCTTCCAAGGGAGTTCTGTTCTATGGACCTCCT
GGCTGTGGGAAAACTTTGTTGGCCAAAGCCATTGCTAATGAATGCCAGGCCAACTTCATC
TCCATCAAGGGTCCTGAGCTGCTCACCATGTGGTTTGGGGAGTCTGAGGCCAATGTCAGA
GAAATCTTTGACAAGGCCCGCCAAGCTGCCCCCTGTGTGCTATTCTTTGATGAGCTGGAT
TCGATTGCCAAGGCTCGTGGAGGTAACATTGGAGATGGTGGTGGGGCTGCTGACCGAGTC
ATCAACCAGATCCTGACAGAAATGGATGGCATGTCCACAAAAAAAAATGTGTTCATCATT
GGCGCTACCAACCGGCCTGACATCATTGATCCTGCCATCCTCAGACCTGGCCGTCTTGAT
CAGCTCATCTACATCCCACTTCCTGATGAGAAGTCCCGTGTTGCCATCCTCAAGGCTAAC
CTGCGCAAGTCCCCAGTTGCCAAGGATGTGGACTTGGAGTTCCTGGCTAAAATGACTAAT
GGCTTCTCTGGAGCTGACCTGACAGAGATTTGCCAGCGTGCTTGCAAGCTGGCCATCCGT
GAATCCATCGAGAGTGAGATTAGGCGAGAACGAGAGAGGCAGACAAACCCATCAGCCATG
GAGGTAGAAGAGGATGATCCAGTGCCTGAGATCCGTCGAGATCACTTTGAAGAAGCCATG
CGCTTTGCGCGCCGTTCTGTCAGTGACAATGACATTCGGAAGTATGAGATGTTTGCCCAG
ACCCTTCAGCAGAGTCGGGGCTTTGGCAGCTTCAGATTCCCTTCAGGGAACCAGGGTGGA
GCTGGCCCCAGTCAGGGCAGTGGAGGCGGCACAGGTGGCAGTGTATACACAGAAGACAAT
GATGATGACCTGTATGGCTAA

Clone variation with respect to NM_007126.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_007126 unedited
NNGGGTCAGATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGTCGCTG
CCGCTGCCGCTGCCACTGCCACTGCCACCTCGCGGATCAGGAGCCAGCGTTGTTCGCCCG
ACGCCTCGCTGCCGGTGGGAGGAAGCGAGAGGGAAGCCGCTTGCGGGTTTGTCGCCGCTG
CTCGCCCACCGCCTGGAAGAGCCGAGCCCCGGCCCAGTCGGTCGCTTGCCACCGCTCGTA
GCCGTTACCCGCGGGCCGCCACAGCCGCCGGCCGGGAGAGGCGCGCGCCATGGCTTCTGG
AGCCGATTCAAAAGGTGATGACCTATCAACAGCCATTCTCAAACAGAAGAACCGTCCCAA
TCGGTTAATTGTTGATGAAGCCATCAATGAGGACAACAGTGTGGTGTCCTTGTCCCAGCC
CAAGATGGATGAATTGCAGTTGTTCCGAGGTGACACAGTGTTGCTGAAAGGAAAGAAGAG
ACGAGAAGCTGTTTGCATCGTCCTTTCTGATGATACTTGTTCTGATGAGAAGATTCGGAT
GAATAGAGTTGTTCGGAATAACCTTCGTGTACGCCTAGGGGATGTCATCAGCATCCAGCC
ATGCCCTGATGTGAAGTACGGCAAACGTATCCATGTGCTGCCCATTGATGACACAGTGGA
AGGCATTACTGGTAATCTCTTCGAGGTATACCTTAAGCCGTACTTCCTGGAAGCGTATCG
ACCCATCCGGAAAGGAGACATTNTTCTTGTCCGTGGTGGGATGCGTGCTGTGGAGTTCAA
AGTGGTGGAAACAGATCCTAGCCCTTATTGCATTGNTGCTCCGACACAGTGATCACTGCG
AAGGNGAGCCTATCANACGAGAGATGAAGGANAGTCCTTGATGAANTAGGTATGATGACA
TGGNTGGT
3' Read Nucleotide Sequence
>OriGene 3' read for NM_007126 unedited
NGGGCATTGNNAGTCGCTTTTCATTTATCGCTTTTGTTTTGTATTTTTGGCAACAGAAAA
CCCCCTGTCCANAGTCAGACTGTAGCTGAACTGTTCAGACTGGAGAATGGAGCAGGCTGT
GGGCGCACCCCTGGTCCCTCTCCTGGGCAAGCGCCCCCACCCCCAGGGAACAAGGTCCAG
GCAGGCCAGCTCACTGCACGCTGGCCACCACCACTTAGCCATACAGGTCATCATCATTGT
CTTCTGTGTATACACTGCCACCTGTGCCGCCTCCACTGCCCTGACTGGGGCCAGCTCCAC
CCTGGTTCCCTGAAGGGAATCTGAAGCTGCCAAAGCCCCGACTCTGCTGAAGGGTCTGGG
CAAACATCTCATACTTCCGAATGTCATTGTCACTGACAGAACGGCGCGCAAAGCGCATGG
CTTCTTCAAAGTGATCTCGACGGATCTCAGGCACTGGATCATCCTCTTCTACCTCCATGG
CTGATGGGTTTGTCTGCCTCTCTCGTTCTCGCCTAATCTCACTCTCGATGGATTCACGGA
TGGCCAGCTTGCAAGCACGCTGGCAAATCTCTGTCAGGTCAGCTCCAGAGAAGCCATTAG
TCATTTTAGCCAGGAACTCCAAGTCCACATCCTTGGCAACTGGGGACTTGCGCANGTTAG
CCTTGAGGATGGGGCACACGGACTTCTCATCANGAAGTGGGATGTAGATGAGCTGATCAA
GACNGCCAGGTCTGANGATGGCAGGATCAATGATGTCAGGCCCGTTGGTAGCGCCCATGA
TGAACACATTTTTTTTTGTGGACATGCCATCCATTTNCTGTCAGGATCTGGNTTGATGAC
TCGTCAGCAGCCCCACCACATCTTCAATGTACCCTCACGAGCCTTGGGGCATCGATCCAG
CTCATCAAAGATAGCCCACAAGGGGCAGCCTG
Restriction Sites NotI-NotI |
ACCN NM_007126
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007126.2, NP_009057.1
RefSeq Size 3198 bp
RefSeq ORF 2421 bp
Locus ID 7415
UniProt ID P55072
Cytogenetics 9p13.3
Domains AAA, cdc48_N
Summary This gene encodes a member of the AAA ATPase family of proteins. The encoded protein plays a role in protein degradation, intracellular membrane fusion, DNA repair and replication, regulation of the cell cycle, and activation of the NF-kappa B pathway. This protein forms a homohexameric complex that interacts with a variety of cofactors and extracts ubiquitinated proteins from lipid membranes or protein complexes. Mutations in this gene cause IBMPFD (inclusion body myopathy with paget disease of bone and frontotemporal dementia), ALS (amyotrophic lateral sclerosis) and Charcot-Marie-Tooth disease in human patients. provided by RefSeq, Aug 2017
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Documents
Resources
"NM_007126" in other vectors (6)
SKU Description Size Price
RC211130 VCP (Myc-DDK-tagged)-Human valosin containing protein (VCP) 10 ug
$738.00
RC211130L1 Lenti ORF clone of Human valosin containing protein (VCP), Myc-DDK-tagged 10 ug
$1,038.00
RC211130L2 Lenti ORF clone of Human valosin containing protein (VCP), mGFP tagged 10 ug
$1,038.00
RC211130L3 Lenti ORF clone of Human valosin containing protein (VCP), Myc-DDK-tagged 10 ug
$1,038.00
RC211130L4 Lenti ORF clone of Human valosin containing protein (VCP), mGFP tagged 10 ug
$1,038.00
RG211130 VCP (tGFP-tagged) - Human valosin containing protein (VCP) 10 ug
$789.00 $938.00

file-alt Citations

Transgenic mice expressing mutant forms VCP/p97 recapitulate the full spectrum of IBMPFD including degeneration in muscle, brain and bone
Custer, Neumann, Lu et al
Hum Mol Genet (2010) 19 (9), 1741-55 DOI: 10.1093/hmg/ddq050
Cited by 208
AI Summary: In this study, OriGene’s human VCP/p97 cDNA clones were used to generate transgenic mice expressing mutant forms of VCP, enabling the researchers to model and investigate the full spectrum of IBMPFD pathology in muscle, brain, and bone.
Showing 1-1 of 1 papers.
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