ASS1 (NM_054012) Human Untagged Clone

SKU SC124908

ASS1 (untagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ASS1
• Synonyms: ASS; CTLN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124908 sequence for NM_054012 edited (data generated by NextGen Sequencing); ATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGACACCTCGTGCATC; CTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGCCAACATTGGCCAG; AAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGGGGCCAAAAAGGTGTTC; ATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCCGGCCATCCAGTCCAGC; GCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAGGCCCTGCATCGCCCGC; AAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCACGGCGCCACAGGA; AAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACTGGCCCCCCAGATAAAG; GTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAAGGGCCGCAATGACCTG; ATGGAGTACGCAAAGCAACACGGGATTCCCATCCCGGTCACTCCCAAGAACCCGTGGAGC; ATGGATGAGAACCTCATGCACATCAGCTACGAGGCTGGAATCCTGGAGAACCCCAAGAAC; CAAGCGCCTCCAGGTCTCTACACGAAGACCCAGGACCCAGCCAAAGCCCCCAACACCCCT; GACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGAAGGTGACCAACGTCAAGGAT; GGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAACGAAGTCGCGGGCAAG; CATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT; ATCTACGAGACCCCAGCAGGCACCATCCTTTACCATGCTCATTTAGACATCGAGGCCTTC; ACCATGGACCGGGAAGTGCGCAAAATCAAACAAGGCCTGGGCTTGAAATTTGCTGAGCTG; GTGTATACCGGTTTCTGGCACAGCCCTGAGTGTGAATTTGTCCGCCACTGCATCGCCAAG; TCCCAGGAGCGAGTGGAAGGGAAAGTGCAGGTGTCCGTCCTCAAGGGCCAGGTGTACATC; CTCGGCCGGGAGTCCCCACTGTCTCTCTACAATGAGGAGCTGGTGAGCATGAACGTGCAG; GGTGATTATGAGCCAACTGATGCCACCGGGTTCATCAACATCAATTCCCTCAGGCTGAAG; GAATATCATCGTCTCCAGAGCAAGGTCACTGCCAAATAG; ; Clone variation with respect to NM_054012.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_054012 unedited; TCACAATTTNGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCCACCGCT; GCCCGAGCCCGACGCTATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCT; GGACACCTCGTGCATCCTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCT; GGCCAACATTGGCCAGAAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGG; GGCCAAAAAGGTGTTCATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCC; GGCCATCCAGTCCAGCGCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAG; GCCCTGCATCGCCCGCAAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTC; CCACGGCGCCACAGGAAAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACT; GGCCCCCCAGATAAAGGTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAA; GGGCCGCAATGACCTGATGGAGTACGCAAAGCAACACGGGATTCCCATCCCGGTCACTCC; CAAGAACCCGTGGAGCATGGATGAGAACCTCATGCACATCAGCTACGAGGCTGGAATCCT; GGAGAACCCCAAGAACCAAGCGCCTCCAGGTCTCTACACGAAGACCCAGGACCCAGCCAA; AGCCCCCAACACCCCTGACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGANAGT; GACCAACGTCAAGGATGGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAA; CGAAGTCGCGGGCAAGCATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTTCATGG; NAATGAGTCCCGAGTATCTACGAGACCCAGCAGGCACATCCTTTACATGCTCATTTAGAC; TCGAGCCTTCACCA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_054012 unedited; CTTTGGTACGTAGCCGCATACTAGGTTCAGTTTTATTTTTTTTTTTTTTTTTTTTTTTCT; CTTTTAATTGTCATTTTTATAGCTCCCCACCGCAGCTGCCCCCCACCCTTCCCTTCGATG; ACAACGTTTGCAGGCTTCAGGGGGACCAGGGAACAAAGCTGGGGCCTGGCAGCCCCACTA; CGCTGCCAGCCGGGGAGAACAAGTCACAATTACTAATTATCACAACAATTAGCGCCTGTA; CTTGGGGGATCTGCAAATTGAGGAGGCCCCAGCTCCTCATTGTACACGGGTCTATTTGGC; AGTGACCTTGCTCTGGAGACGATGATATTCCTTCAGCCTGAGGGAATTGATGTTGATGAA; CCCGGTGGCATCAGTTGGCTCATAATCACCCTGCACGTTCATGCTCACCAGCTCCTCATT; GTAGAGAGACAGTGGGGACTCCCGGCCGAGGATGTACACCTGGCCCTTGAGGACGGACAC; CTGCACTTTCCCTTCCACTCGCTCCTGGGACTTGGCGATGCAGTGGCGGACAAATTCACA; CTCAGGGCTGTGCCAGAAACCGGTATACACCAGCTCAGCAAATTTCAAGCCCAGGCCTTG; TTTGATTTTGCGCACTTCCCGGTCCATGGTGAAGGCCTCGATGTCTAAATGAGCATGGTA; AAGGATGGTGCCTGCTGGGGTCTCGTAGATACCTCGGGACTTCATTCCAATGAAGCGGTT; CTCCACGATGTCAATACGGCCCACGCCATGCTTGCCCGCGACTTCGTTCAGGTACATGAA; GAGCTCCAAGGAGTCTGGTGGGTGGTGCCATCCTTGACGTTGGTCACCTTCCAGGGACCC; CTTTTTTTGACTCGATCTCNAGAATGTCAGGNGTGTTNGGGGGCTTTGGCTGGGTCCTGG; GTCTTCGTGTANAGACCTGGAGGCGCTTGGGTCTNGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_054012
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_054012.2, NP_446464.1
• RefSeq Size: 1631 bp
• RefSeq ORF: 1239 bp
• Locus ID: 445
• UniProt ID: P00966
• Cytogenetics: 9q34.11
• Domains: Arginosuc_synth
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, Arginine and proline metabolism, aspartate and glutamate metabolism, Metabolic pathways
• Summary: The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2012]; Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.