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ASS1 (NM_054012) Human Untagged Clone

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SKU
SC124908
ASS1 (untagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 2
  $457.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ASS1
Synonyms ASS; CTLN1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC124908 sequence for NM_054012 edited (data generated by NextGen Sequencing)
ATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCTGGACACCTCGTGCATC
CTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCTGGCCAACATTGGCCAG
AAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGGGGCCAAAAAGGTGTTC
ATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCCGGCCATCCAGTCCAGC
GCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAGGCCCTGCATCGCCCGC
AAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTCCCACGGCGCCACAGGA
AAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACTGGCCCCCCAGATAAAG
GTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAAGGGCCGCAATGACCTG
ATGGAGTACGCAAAGCAACACGGGATTCCCATCCCGGTCACTCCCAAGAACCCGTGGAGC
ATGGATGAGAACCTCATGCACATCAGCTACGAGGCTGGAATCCTGGAGAACCCCAAGAAC
CAAGCGCCTCCAGGTCTCTACACGAAGACCCAGGACCCAGCCAAAGCCCCCAACACCCCT
GACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGAAGGTGACCAACGTCAAGGAT
GGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAACGAAGTCGCGGGCAAG
CATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTCATTGGAATGAAGTCCCGAGGT
ATCTACGAGACCCCAGCAGGCACCATCCTTTACCATGCTCATTTAGACATCGAGGCCTTC
ACCATGGACCGGGAAGTGCGCAAAATCAAACAAGGCCTGGGCTTGAAATTTGCTGAGCTG
GTGTATACCGGTTTCTGGCACAGCCCTGAGTGTGAATTTGTCCGCCACTGCATCGCCAAG
TCCCAGGAGCGAGTGGAAGGGAAAGTGCAGGTGTCCGTCCTCAAGGGCCAGGTGTACATC
CTCGGCCGGGAGTCCCCACTGTCTCTCTACAATGAGGAGCTGGTGAGCATGAACGTGCAG
GGTGATTATGAGCCAACTGATGCCACCGGGTTCATCAACATCAATTCCCTCAGGCTGAAG
GAATATCATCGTCTCCAGAGCAAGGTCACTGCCAAATAG

Clone variation with respect to NM_054012.3
5' Read Nucleotide Sequence
>OriGene 5' read for NM_054012 unedited
TCACAATTTNGTAATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCCACCGCT
GCCCGAGCCCGACGCTATGTCCAGCAAAGGCTCCGTGGTTCTGGCCTACAGTGGCGGCCT
GGACACCTCGTGCATCCTCGTGTGGCTGAAGGAACAAGGCTATGACGTCATTGCCTATCT
GGCCAACATTGGCCAGAAGGAAGACTTCGAGGAAGCCAGGAAGAAGGCACTGAAGCTTGG
GGCCAAAAAGGTGTTCATTGAGGATGTCAGCAGGGAGTTTGTGGAGGAGTTCATCTGGCC
GGCCATCCAGTCCAGCGCACTGTATGAGGACCGCTACCTCCTGGGCACCTCTCTTGCCAG
GCCCTGCATCGCCCGCAAACAAGTGGAAATCGCCCAGCGGGAGGGGGCCAAGTATGTGTC
CCACGGCGCCACAGGAAAGGGGAACGATCAGGTCCGGTTTGAGCTCAGCTGCTACTCACT
GGCCCCCCAGATAAAGGTCATTGCTCCCTGGAGGATGCCTGAATTCTACAACCGGTTCAA
GGGCCGCAATGACCTGATGGAGTACGCAAAGCAACACGGGATTCCCATCCCGGTCACTCC
CAAGAACCCGTGGAGCATGGATGAGAACCTCATGCACATCAGCTACGAGGCTGGAATCCT
GGAGAACCCCAAGAACCAAGCGCCTCCAGGTCTCTACACGAAGACCCAGGACCCAGCCAA
AGCCCCCAACACCCCTGACATTCTCGAGATCGAGTTCAAAAAAGGGGTCCCTGTGANAGT
GACCAACGTCAAGGATGGCACCACCCACCAGACCTCCTTGGAGCTCTTCATGTACCTGAA
CGAAGTCGCGGGCAAGCATGGCGTGGGCCGTATTGACATCGTGGAGAACCGCTTTCATGG
NAATGAGTCCCGAGTATCTACGAGACCCAGCAGGCACATCCTTTACATGCTCATTTAGAC
TCGAGCCTTCACCA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_054012 unedited
CTTTGGTACGTAGCCGCATACTAGGTTCAGTTTTATTTTTTTTTTTTTTTTTTTTTTTCT
CTTTTAATTGTCATTTTTATAGCTCCCCACCGCAGCTGCCCCCCACCCTTCCCTTCGATG
ACAACGTTTGCAGGCTTCAGGGGGACCAGGGAACAAAGCTGGGGCCTGGCAGCCCCACTA
CGCTGCCAGCCGGGGAGAACAAGTCACAATTACTAATTATCACAACAATTAGCGCCTGTA
CTTGGGGGATCTGCAAATTGAGGAGGCCCCAGCTCCTCATTGTACACGGGTCTATTTGGC
AGTGACCTTGCTCTGGAGACGATGATATTCCTTCAGCCTGAGGGAATTGATGTTGATGAA
CCCGGTGGCATCAGTTGGCTCATAATCACCCTGCACGTTCATGCTCACCAGCTCCTCATT
GTAGAGAGACAGTGGGGACTCCCGGCCGAGGATGTACACCTGGCCCTTGAGGACGGACAC
CTGCACTTTCCCTTCCACTCGCTCCTGGGACTTGGCGATGCAGTGGCGGACAAATTCACA
CTCAGGGCTGTGCCAGAAACCGGTATACACCAGCTCAGCAAATTTCAAGCCCAGGCCTTG
TTTGATTTTGCGCACTTCCCGGTCCATGGTGAAGGCCTCGATGTCTAAATGAGCATGGTA
AAGGATGGTGCCTGCTGGGGTCTCGTAGATACCTCGGGACTTCATTCCAATGAAGCGGTT
CTCCACGATGTCAATACGGCCCACGCCATGCTTGCCCGCGACTTCGTTCAGGTACATGAA
GAGCTCCAAGGAGTCTGGTGGGTGGTGCCATCCTTGACGTTGGTCACCTTCCAGGGACCC
CTTTTTTTGACTCGATCTCNAGAATGTCAGGNGTGTTNGGGGGCTTTGGCTGGGTCCTGG
GTCTTCGTGTANAGACCTGGAGGCGCTTGGGTCTNGC
Restriction Sites NotI-NotI |
ACCN NM_054012
Insert Size 1500 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_054012.2, NP_446464.1
RefSeq Size 1631 bp
RefSeq ORF 1239 bp
Locus ID 445
UniProt ID P00966
Cytogenetics 9q34.11
Domains Arginosuc_synth
Protein Families Druggable Genome
Protein Pathways Alanine, Arginine and proline metabolism, aspartate and glutamate metabolism, Metabolic pathways
Summary The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Aug 2012
Transcript Variant: This variant (2) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein.
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Documents
Resources
"NM_054012" in other vectors (7)
SKU Description Size Price
RC201130 ASS1 (Myc-DDK-tagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 2 10 ug
$457.00
RC201130L1 Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC201130L2 Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 2, mGFP tagged 10 ug
$757.00
RC201130L3 Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC201130L4 Lenti ORF clone of Human argininosuccinate synthase 1 (ASS1), transcript variant 2, mGFP tagged 10 ug
$757.00
RG201130 ASS1 (tGFP-tagged) - Human argininosuccinate synthase 1 (ASS1), transcript variant 2 10 ug
$489.00 $657.00
SC320331 ASS1 (untagged)-Human argininosuccinate synthase 1 (ASS1), transcript variant 2 10 ug
$457.00

file-alt Citations

Nutritional stress and arginine auxotrophy confer high sensitivity to chloroquine toxicity in mesothelioma cells
Battisti, Valente, Albonici et al
Am. J. Respir. Cell Mol. Biol. (2012) 46 (4), 498-506 DOI: 10.1165/rcmb.2011-0195OC
Cited by 3
AI Summary: OriGene’s ASS1 expression vector (NM_054012.3) was used to overexpress argininosuccinate synthetase in mesothelioma cells, enabling the study of how ASS1 expression modulates sensitivity to chloroquine toxicity under nutritional stress.
Snippet: NM_054012.3;OriGene Technologies, Rockville, MD), or with an empty vector, using the jetPEI reagent (Polyplus transfection, Illkirch, France). ... Indeed, the recombinant proteins need to be pegylated to increase their otherwise extremely short half-life and decrease their
Showing 1-1 of 1 papers.
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