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EEF1A1 (NM_001402) Human Untagged Clone

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SKU
SC124558
EEF1A1 (untagged)-Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1)
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol EEF1A1
Synonyms CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EF-Tu; EF1A; GRAF-1EF; LENG7; PTI1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC124558 sequence for NM_001402 edited (data generated by NextGen Sequencing)
ATGGGAAAGGAAAAGACTCATATCAACATTGTCGTCATTGGACACGTAGATTCGGGCAAG
TCCACCACTACTGGCCATCTGATCTATAAATGCGGTGGCATCGACAAAAGAACCATTGAA
AAATTTGAGAAGGAGGCTGCTGAGATGGGAAAGGGCTCCTTCAAGTATGCCTGGGTCTTG
GATAAACTGAAAGCTGAGCGTGAACGTGGTATCACCATTGATATCTCCTTGTGGAAATTT
GAGACCAGCAAGTACTATGTGACTATCATTGATGCCCCAGGACACAGAGACTTTATCAAA
AACATGATTACAGGGACATCTCAGGCTGACTGTGCTGTCCTGATTGTTGCTGCTGGTGTT
GGTGAATTTGAAGCTGGTATCTCCAAGAATGGGCAGACCCGAGAGCATGCCCTTCTGGCT
TACACACTGGGTGTGAAACAACTAATTGTCGGTGTTAACAAAATGGATTCCACTGAGCCA
CCCTACAGCCAGAAGAGATATGAGGAAATTGTTAAGGAAGTCAGCACTTACATTAAGAAA
ATTGGCTACAACCCCGACACAGTAGCATTTGTGCCAATTTCTGGTTGGAATGGTGACAAC
ATGCTGGAGCCAAGTGCTAACATGCCTTGGTTCAAGGGATGGAAAGTCACCCGTAAGGAT
GGCAATGCCAGTGGAACCACGCTGCTTGAGGCTCTGGACTGCATCCTACCACCAACTCGT
CCAACTGACAAGCCCTTGCGCCTGCCTCTCCAGGATGTCTACAAAATTGGTGGTATTGGT
ACTGTTCCTGTTGGCCGAGTGGAGACTGGTGTTCTCAAACCCGGTATGGTGGTCACCTTT
GCTCCAGTCAACGTTACAACGGAAGTAAAATCTGTCGAAATGCACCATGAAGCTTTGAGT
GAAGCTCTTCCTGGGGACAATGTGGGCTTCAATGTCAAGAATGTGTCTGTCAAGGATGTT
CGTCGTGGCAACGTTGCTGGTGACAGCAAAAATGACCCACCAATGGAAGCAGCTGGCTTC
ACTGCTCAGGTGATTATCCTGAACCATCCAGGCCAAATAAGCGCCGGCTATGCCCCTGTA
TTGGATTGCCACACGGCTCACATTGCATGCAAGTTTGCTGAGCTGAAGGAAAAGATTGAT
CGCCGTTCTGGTAAAAAGCTGGAAGATGGCCCTAAATTCTTGAAGTCTGGTGATGCTGCC
ATTGTTGATATGGTTCCTGGCAAGCCCATGTGTGTTGAGAGCTTCTCAGACTATCCACCT
TTGGGTCGCTTTGCTGTTCGTGATATGAGACAGACAGTTGCGGTGGGTGTCATCAAAGCA
GTGGACAAGAAGGCTGCTGGAGCTGGCAAGGTCACCAAGTCTGCCCAGAAAGCTCAGAAG
GCTAAATGA

Clone variation with respect to NM_001402.5
5' Read Nucleotide Sequence
>OriGene 5' read for NM_001402 unedited
NTTCGGAATTGAAACCGATTACTATAGGCGGACGCCCAATTCGCACGAGGGCCGCCATAC
ACAGGTGTCGTGAAACTACCCCCAAAGCCAAAAGGGAAAGGAAAAGACTCATATCAACAT
TGTCGTCATTGGACACGTAGATTCGGGCAAGTCCACCACTACTGGCCATCTGATCTATAA
ATGCGGTGGCATCGACAAAAGATTTTTTGTAAAAATTTGAGAAGGAGGCTGCTGAGATGG
GAAAGGGCTCCTTCAAGTATGCCTGGGTCTTGGATAAACTGAAAGCTGAGCGTGAACGTG
GTATCACCATTGATATCTCCTTGTGGAAATTTGAGACCAGCAAGTACTATGTGACTATCA
TTGATGCCCCAGGACACAGAGACTTTATCAAAAACATGATTACAGGGACATCTCAGGCTG
ACTGTGCTGTCCTGATTGTTGCTGCTGGTGTTGGTGAATTTGAAGCTGGTATCTCCAAGA
ATGGGCAGACCCGAGAGCATGCCCTTCTGGCTTACACACTGGGTGTGAAACAACTAATTG
TCGGTGTTAACAAAATGGATTCCACTGAGCCACCCTACAGCCAGAAGAGATATGAGGAAA
TTGTTAAGGAAGTCAGCACTTACATTAAGAAAATTGGCTACAACCCCGACACAGTAGCAT
TTGTGCCAATTTCTGGTTGGAATGGTGACAACATGCTGGAGCCAAGTGCTAACATGCCTT
GGTTCAAGGGATGGAAAGTCACCCGTAAAGATGGCCATGCCAGTGGAAACACGCCTGCTT
GAGCTCTGGACTGCATCCTACCACCAACTCGTCCCACTGACAAGCCCCTTGCGCCCTGCC
TCTCCAGGATGTCTACAAAATTGGTGGCNATTGGTACTGTTTCCTGTTGGNCCGAGTGAA
AACTGCNTGTCTTCAA
3' Read Nucleotide Sequence
>OriGene 3' read for NM_001402 unedited
ATGGCAACTTTCCAGGNCCAGGNANAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGG
ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
TTTTTCTCATTTAACTTTTTTAATGGGTCTCAAAATTCTGTGACAAATTTTTGGTCAAGT
TGTTTCCATTAAAAAGTACTGATTTTAAAAACTAATAACTTAAAACTGCCACACGCAAAA
AAGAAAACCAAAGTGGTCCACAAAACATTCTCCTTTCCTTCTGAAGGTTTTACGATGCAT
TGTTATCATTAACCAGTCTTTTACTACTAAACTTAAATGGCCAATTGAAACAAACAGTTC
TGAGACCGTTCTTCCACCACTGATTAAGAGTGGGGTGGCAGGTATTAGGGATAATATTCA
TTTAGCCTTCTGAGCTTTCTGGGCAGACTTGGTGACCTTGCCAGCTCCAGCAGCCTTCTT
GTCCACTGCTTTGATGACACCCACCGCAACTGTCTGTCTCATATCACGAACAGCAAAGCG
ACCCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGNGGCTTGCCAGGAACCATAT
CAACAATGGCAGCATCACCAGACTTCAAGAATTTAGGGCCATCTTCCAGCTTTTTACCAG
AACGGCGATCAATCTTTTCCTTCAGCTCAGCAAACTTGCATGCAAATGTGAGCCGTGTGG
CAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCANGATAATCACC
TGAGCAGTGAAGCAGCTGCTCCATTGGTGGGTCATTTTTGCTGTCACCAGCACGTGCCAC
GACGACATCCTTGCAGACACATTCTGACATGAGCCCACATTGTCCCAGNA
Restriction Sites NotI-NotI |
ACCN NM_001402
Insert Size 1710 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001402.4, NP_001393.1
RefSeq Size 1837 bp
RefSeq ORF 1389 bp
Locus ID 1915
UniProt ID P68104
Cytogenetics 6q13
Domains GTP_EFTU, GTP_EFTU_D2, GTP_EFTU_D3
Protein Families Druggable Genome
Summary This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001402" in other vectors (4)
SKU Description Size Price
RC209697 EEF1A1 (Myc-DDK-tagged)-Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) 10 ug
$457.00
RC209697L3 Lenti ORF clone of Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), Myc-DDK-tagged 10 ug
$757.00
RC209697L4 Lenti ORF clone of Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1), mGFP tagged 10 ug
$757.00
RG209697 EEF1A1 (tGFP-tagged) - Human eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) 10 ug
$489.00 $657.00

file-alt Citations

Human eukaryotic elongation factor 1A forms oligomers through specific cysteine residues
Liu, Yang, Wang et al
Acta Biochim. Biophys. Sin. (Shanghai) (2015) 47 (12), 1011-7 DOI: 10.1093/abbs/gmv113
Cited by 10
AI Summary: OriGene contributed to this study by providing the human eEF1A1 full length open reading frame (ORF) plasmid (catalog number sc124558), which was used as the amplification template for plasmid construction and mutagenesis experiments.
Snippet: Materials and Methods. Plasmid construction and mutagenesis. The human eEF1A1 full length open reading frame (ORF) was purchased from Origene (sc124558;Rockville, USA) and used as the amplification template. For ... Cite SaveSaving
Use of phage display to identify novel mineralocorticoid receptor-interacting proteins
Yang, Fuller, Morgan et al
Mol. Endocrinol. (2014)
Cited by 38
AI Summary: OriGene products, specifically the pCMV6-XL5 vector and pCMV6-XL5 expression plasmids for EEF1A1, SSRP1, and XRCC6, were used to express and study the roles of these candidate MR-interacting proteins in the identification and characterization of novel mineralocorticoid receptor coregulators.
Snippet: CCCGGGAATTTCCGGCGATACAGT) primers to introduce the amino acids. pCMV6-XL5 vector and pCMV6-XL5- EEF1A1, -SSRP1, and –XRCC6 were purchased from OriGene (Rockville). Expression plasmids of MR (PRshMR), GR ... Cite SaveSaving
Showing 1-2 of 2 papers.
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