NALP12 (NLRP12) (NM_144687) Human Untagged Clone

SKU SC124283

NLRP12 (untagged)-Human NLR family, pyrin domain containing 12 (NLRP12), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: NALP12
• Synonyms: CLR19.3; FCAS2; NALP12; PAN6; PYPAF7; RNO; RNO2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_144687 edited; CCCCATGCTACGAACCGCAGGCAGGGACGGCCTCTGTCGCCTGTCCACCTACTTGGAAGA; ACTCGAGGCTGTGGAACTGAAGAAGTTCAAGTTATACCTGGGGACCGCGACAGAGCTGGG; AGAAGGCAAGATCCCCTGGGGAAGCATGGAGAAGGCCGGTCCCCTGGAAATGGCCCAGCT; GCTCATCACCCACTTCGGGCCAGAGGAGGCCTGGAGGTTGGCTCTCAGCACCTTTGAGCG; GATAAACAGGAAGGACCTGTGGGAGAGAGGACAGAGAGAGGACCTGGTGAGGGATACCCC; ACCTGGTGGCCCGTCCTCACTTGGGAACCAGTCAACATGCCTTCTGGAAGTCTCTCTTGT; CACTCCAAGAAAAGATCCCCAGGAAACCTACAGGGACTATGTCCGCAGGAAATTCCGGCT; CATGGAAGACCGCAATGCGCGCCTAGGGGAATGTGTCAACCTCAGCCACCGGTACACCCG; GCTCCTGCTGGTGAAGGAGCACTCAAACCCCATGCAGGTCCAGCAGCAGCTTCTGGACAC; AGGCCGGGGACACGCGAGGACCGTGGGACACCAGGCTAGCCCCATCAAGATAGAGACCCT; CTTTGAGCCAGACGAGGAGCGCCCCGAGCCACCGCGCACCGTGGTCATGCAAGGCGCGGC; AGGGATAGGCAAGTCCATGCTGGCACACAAGGTGATGCTGGACTGGGCGGACGGGAAGCT; CTTCCAAGGCAGATTTGATTATCTCTTCTACATCAACTGCAGGGAGATGAACCAGAGTGC; CACGGAATGCAGCATGCAAGACCTCATCTTCAGCTGCTGGCCTGAGCCCAGCGCGCCTCT; CCAGGAGCTCATCCGAGTTCCCGAGCGCCTCCTTTTCATCATCGACGGCTTCGATGAGCT; CAAGCCTTCTTTCCACGATCCTCAGGGACCCTGGTGCCTCTGCTGGGAGGAGAAACGGCC; CACGGAGCTGCTTCTTAACAGCTTAATTCGGAAGAAGCTGCTCCCTGAGCTATCTTTGCT; CATCACCACACGGCCCACGGCTTTGGAGAAGCTCCACCGTCTGCTGGAGCACCCCAGGCA; TGTGGAGATCCTGGGCTTCTCTGAGGCAGAAAGGAAGGAATACTTCTACAAGTATTTCCA; CAATGCAGAGCAGGCGGGCCAAGTCTTCAATTACGTGAGGGACAACGAGCCTCTCTTCAC; CATGTGCTTGGTCCCCCTGGTGTGCTGGGTGGTGTGTACCTGCCTCCAGCAGCAGCTGGA; GGGTGGGGGGCTGTTGAGACAGACGTCCAGGACCACCACTGCAGTGTACATGCTCTACCT; GCTGAGTCTGATGCAACCCAAGCCGGGGGCCCCGCGCCTCCAGCCCCCACCCAACCAGAG; AGGGTTGTGCTCCTTGGCGGCAGATGGGCTCTGGAATCAGAAAATCCTATTTGAGGAGCA; GGACCTCCGGAAGCACGGCCTAGACGGGGAAGACGTCTCTGCCTTCCTCAACATGAACAT; CTTCCAGAAGGACATCAACTGTGAGAGGTACTACAGCTTCATCCACTTGAGTTTCCAGGA; ATTCTTTGCAGCTATGTACTATATCCTGGACGAGGGGGAGGGCGGGGCAGGCCCAGACCA; GGACGTGACCAGGCTGTTGACCGAGTACGCGTTTTCTGAAAGGAGCTTCCTGGCACTCAC; CAGCCGCTTCCTGTTTGGACTCCTGAACGAGGAGACCAGGAGCCACCTGGAGAAGAGTCT; CTGCTGGAAGGTCTCGCCGCACATCAAGATGGACCTGTTGCAGTGGATCCAAAGCAAAGC; TCAGAGCGACGGCTCCACCCTGCAGCAGGGCTCCTTGGAGTTCTTCAGCTGCTTGTACGA; GATCCAGGAGGAGGAGTTTATCCAGCAGGCCCTGAGCCACTTCCAGGTGATCGTGGTCAG; CAACATTGCCTCCAAGATGGAGCACATGGTCTCCTCGTTCTGTCTGAAGCGCTGCAGGAG; CGCCCAGGTGCTGCACTTGTATGGCGCCACCTACAGCGCGGACGGGGAAGACCGCGCGAG; GTGCTCCGCAGGAGCGCACACGCTGTTGGTGCAGCTACCAGAGAGGACCGTTCTGCTGGA; CGCCTACAGTGAACATCTGGCAGCGGCCCTGTGCACCAATCCAAACCTGATAGAGCTGTC; TCTGTACCGAAATGCCCTGGGCAGCCGGGGGGTGAAGCTGCTCTGTCAAGGACTCAGACA; CCCCAACTGCAAACTTCAGAACCTGAGGCTGAAGAGGTGCCGCATCTCCAGCTCAGCCTG; CGAGGACCTCTCTGCAGCTCTCATAGCCAATAAGAATTTGACAAGGATGGATCTCAGTGG; CAACGGCGTTGGATTCCCAGGCATGATGCTGCTTTGCGAGGGCCTGCGGCATCCCCAATG; CAGGCTGCAGATGATTCAGTTGAGGAAGTGTCAGCTGGAGTCCGGGGCTTGTCAGGAGAT; GGCTTCTGTGCTCGGCACCAACCCACATCTGGTTGAGTTGGACCTGACAGGAAATGCACT; GGAGGATTTGGGCCTGAGGTTACTATGCCAGGGACTGAGGCACCCAGTCTGCAGACTACG; GACTTTGTGGCTGAAGATCTGCCGCCTCACTGCTGCTGCCTGTGACGAGCTGGCCTCAAC; TCTCAGTGTGAACCAGAGCCTGAGAGAGCTGGACCTGAGCCTGAATGAGCTGGGGGACCT; CGGGGTGCTGCTGCTGTGTGAGGGCCTCAGGCATCCCACGTGCAAGCTCCAGACCCTGCG; GCTGGATAGCTGTGGCCTCACAGCCAAGGCTTGTGAGAATCTTTACTTCACCCTGGGGAT; CAACCAGACCTTGACCGACCTTTACCTGACCAACAACGCCCTAGGGGACACAGGTGTCCG; ACTGCTTTGCAAGCGGCTGAGCCATCCTGGCTGCAAACTCCGAGTCCTCTGGTTATTTGG; GATGGACCTGAATAAAATGACCCACAGTAGGTTGGCAGCGCTTCGAGTAACAAAACCTTA; TTTGGACATTGGCTGCTGAATGGTCCTATCTGCTGGCTCTCCCCTGAGATCTGGACAGAG; GAAGATGGGAGGGTGCTCATCACCCCCCCAGCATAATGATCAGCCTCCTTCCTAGAGACA; GACTCATGCAGATTGAGATCAAAAGTCCCTCTGCTTGGGATCAAATTAATGTTTGACAGA; GCTGGCCAGGCGTGGTGGCTCATGTATGTAATCCTAGCACTTCGAGAGGCCGAGGCAGGT; GGATCACGAGGTCAGGAGTTTGAGATTAGCCTGGCCAAGATGGTGAAACCCTGTCTCTAC; TAAAAATAAAAAAAAATTAGCCAGG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_144687 unedited; NGGCAAGTCAAAATTTGTATACGACTCCTATAGGCGGCCGCNGATTCGGCACCAGCCCCA; TGCTACGAACCGCAGGCAGGGACGGCCTCTGTCGCCTGTCCACCTACTTGGAAGAACTCG; AGGCTGTGGAACTGAAGAAGTTCAAGTTATACCTGGGGACCGCGACAGAGCTGGGAGAAG; GCAAGATCCCCTGGGGAAGCATGGAGAAGGCCGGTCCCCTGGAAATGGCCCAGCTGCTCA; TCACCCACTTCGGGCCAGAGGAGGCCTGGAGGTTGGCTCTCAGCACCTTTGAGCGGATAA; ACAGGAAGGACCTGTGGGAGAGAGGACAGAGAGAGGACCTGGTGAGGGATACCCCACCTG; GTGGCCCGTCCTCACTTGGGAACCAGTCAACATGCCTTCTGGAAGTCTCTCTTGTCACTC; CAAGAAAAGATCCCCAGGAAACCTACAGGGACTATGTCCGCAGGAAATTCCGGCTCATGG; AAGACCGCAATGCGCGCCTAGGGGAATGTGTCAACCTCAGCCACCGGTACACCCGGCTCC; TGCTGGTGAAGGAGCACTCAAACCCCATGCAGGTCCAGCAGCAGCTTCTGGACACAGGCC; GGGGACACGCGAGGACCGTGGGACACCAGGCTAGCCCCATCAAGATAGAGACCCTCTTTG; AGCCAGACGAGGAGCGCCCCGAGCCACCGCGCACCGTGGTCATGCAAGGCGCGGCAGGGA; TAGGCAAGTCCATGCTGGCACACAANGTGATGNCTGACTGGGGCGACGGGAAGCTCTTCC; AGGCAGATTTGATTATTCTCTCTACATCAACTGCAGGGAGATGAACCAGAGTGCCACGGA; ATGCAGCATGNCAGACCTCATCTTCAGCTGCTGGCCTGAGCCCACGCGCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_144687 unedited; CCCTCGTGGAGTGATGTTCTTCTTTCTTAGTAAANACAGGGTTTCACCATCTTGTGCCAG; CTAATCTCAAACTCCTGACCTCGTGATCCACCTGCCTCGGCCTCTCGAAGTGCTAGGATT; ACATACATGAGCCACCACGCCTGGCCAGCTCTGTCAAACATTAATTTGATCCCAAGCAGA; GGGACTTTTGATCTCAATCTGCATGAGTCAGTCTCTAGGAAGGAGGCTGATCATTATGCT; GGGGGGGTGATGAGCACCCTCCCATCTTCCTCTGTCCAGATCTCAGGGGAGAGCCAGCAG; ATAGGACCATTCAGCAGCCAATGTCCAAATAAGGTTTTGTTACTCGAAGCGCTGCCAACC; TACTGTGGGTCATTTTATTCAGGTCCATCCCAAATAACCAGAGGACTCGGAGTTTGCAGC; CAGGATGGCTCAACCGCTTGCAAAGCAGTCGGACACCTGTGTCCCCTAGGGCGTTGTTGG; TCAGGTAAAGGTCCGACAAGGTCTGGTTGGTCCCCAGGGTGAAGTAAAGATTCTCACAAG; CCTTGGCTGTGAGGGCACAGCTTTCCAGCCCTAGGGTCTGGACCTTGCACCGTGGGATGC; CCTGAGCCCTCACACAGCAGCAGCGCCCCAGAGGTTCCCCCAAATCAATCAAGGCTCAGG; TCCCGCTCTCTTTAGGCTCTGGTTCACCCTTGTGAGTTTGAGTGCCCGACCGGTTCCAAG; CGAGCAGGCAGTGAGGCTGCCTCCCTTCCTCCCCAAAAGTCTCCCTGTGTGGAGACCCGA; GTGCCCTATATTCCCCGGTACTAGAAACCCGCAGGAGCCAATTACTTCAGTGATATTATC; TGTGAGGGGCACCTAAACCACATAGTGGTTGGACGCCTCACACATAACCCTACTACCTAG; ATGGACCGTTCTATAAATGTACCTTGTCCCAGAGAGATATCATCGCACCCACACATGCGT; AATGGCTTGAAN;
• Restriction Sites: NotI-NotI
• ACCN: NM_144687
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144687.1, NP_653288.1
• RefSeq Size: 3827 bp
• RefSeq ORF: 3186 bp
• Locus ID: 91662
• UniProt ID: P59046
• Cytogenetics: 19q13.42
• Domains: LRR, PAAD_DAPIN
• Summary: This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein, which contains an N-terminal pyrin domain, a NACHT domain, a NACHT-associated domain, and a C-terminus leucine-rich repeat region, functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Mutations in this gene cause familial cold autoinflammatory syndrome type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]; Transcript Variant: This variant (2) uses an alternate splice site in the central coding region, compared to variant 3, resulting in an isoform (2) that is 1 aa shorter than isoform 3.